Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity

Autores
Kolling, Yanina Noralí; Salva, Maria Susana; Zelaya, Hortensia; Alvarez, Susana Beatriz; Villena, Julio Cesar
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The effect of nasally administered Lactobacillus rhamnosus CRL1505 and its peptidoglycan (Pg) on respiratory antiviral immunity triggered by poly(I:C) was studied. Mice were nasally treated with viable (LV) or heat killed (LH) CRL1505 strain or Pg for 2d. Then, these groups and untreated control mice were nasally challenged with 3 daily doses of poly(I:C) (250 μg/mouse). INFγ, IFNβ, IFNα, TNFα, IL6, IL10 in bronchoalveolar lavage (BAL) and serum, expression of CD3, CD4, CD11b, CD11c, CD103, MHCII, IL10 and INFγ in lungs, and lung injury were determined. Poly(I:C) increased the levels of all the evaluated cytokines, accompanied by the recruitment of immune cells into the lung. In addition, increased protein content and LDH activity in BAL and altered lung wet weight:dry weight ratio were observed, indicating increased permeability of the alveolar-capillary barrier and lung injury. LV, LH or Pg administration enhanced INFγ, IFNβ and IL6 production and increased MHCII+CD103+, MHCII+CD11b+ dendritic cells and CD4+INFγ+and CD4+IL10+lymphocytes in lungs. Mice treated with LV, LH or Pg showed lower lung tissue damage. Results show that nasal administration of non-viable Lr1505 or its Pg maintains the probiotic properties of the viable strain and therefore they could be used to beneficially modulate the antiviral inflammatory response in the respiratory mucosa.
Fil: Kolling, Yanina Noralí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Zelaya, Hortensia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXX Annual Scientific Meeting Tucuman Biology Association
Horco Molle
Argentina
Asociación de Biología de Tucumán
Materia
Lactobacillus rhamnosus CRL1505
Peptidoglican
Antiviral respiratory response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258240
Acceder
id CONICETDig_27564e6f0fffd60e13cdaa6f890c3de9
oai_identifier_str oai:ri.conicet.gov.ar:11336/258240
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunityKolling, Yanina NoralíSalva, Maria SusanaZelaya, HortensiaAlvarez, Susana BeatrizVillena, Julio CesarLactobacillus rhamnosus CRL1505PeptidoglicanAntiviral respiratory responsehttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The effect of nasally administered Lactobacillus rhamnosus CRL1505 and its peptidoglycan (Pg) on respiratory antiviral immunity triggered by poly(I:C) was studied. Mice were nasally treated with viable (LV) or heat killed (LH) CRL1505 strain or Pg for 2d. Then, these groups and untreated control mice were nasally challenged with 3 daily doses of poly(I:C) (250 μg/mouse). INFγ, IFNβ, IFNα, TNFα, IL6, IL10 in bronchoalveolar lavage (BAL) and serum, expression of CD3, CD4, CD11b, CD11c, CD103, MHCII, IL10 and INFγ in lungs, and lung injury were determined. Poly(I:C) increased the levels of all the evaluated cytokines, accompanied by the recruitment of immune cells into the lung. In addition, increased protein content and LDH activity in BAL and altered lung wet weight:dry weight ratio were observed, indicating increased permeability of the alveolar-capillary barrier and lung injury. LV, LH or Pg administration enhanced INFγ, IFNβ and IL6 production and increased MHCII+CD103+, MHCII+CD11b+ dendritic cells and CD4+INFγ+and CD4+IL10+lymphocytes in lungs. Mice treated with LV, LH or Pg showed lower lung tissue damage. Results show that nasal administration of non-viable Lr1505 or its Pg maintains the probiotic properties of the viable strain and therefore they could be used to beneficially modulate the antiviral inflammatory response in the respiratory mucosa.Fil: Kolling, Yanina Noralí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Zelaya, Hortensia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Alvarez, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaXXX Annual Scientific Meeting Tucuman Biology AssociationHorco MolleArgentinaAsociación de Biología de TucumánTech Science Press2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258240Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity; XXX Annual Scientific Meeting Tucuman Biology Association; Horco Molle; Argentina; 2013; 42-420327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:07Zoai:ri.conicet.gov.ar:11336/258240instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:07.768CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
title Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
spellingShingle Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
Kolling, Yanina Noralí
Lactobacillus rhamnosus CRL1505
Peptidoglican
Antiviral respiratory response
title_short Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
title_full Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
title_fullStr Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
title_full_unstemmed Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
title_sort Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity
dc.creator.none.fl_str_mv Kolling, Yanina Noralí
Salva, Maria Susana
Zelaya, Hortensia
Alvarez, Susana Beatriz
Villena, Julio Cesar
author Kolling, Yanina Noralí
author_facet Kolling, Yanina Noralí
Salva, Maria Susana
Zelaya, Hortensia
Alvarez, Susana Beatriz
Villena, Julio Cesar
author_role author
author2 Salva, Maria Susana
Zelaya, Hortensia
Alvarez, Susana Beatriz
Villena, Julio Cesar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus rhamnosus CRL1505
Peptidoglican
Antiviral respiratory response
topic Lactobacillus rhamnosus CRL1505
Peptidoglican
Antiviral respiratory response
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The effect of nasally administered Lactobacillus rhamnosus CRL1505 and its peptidoglycan (Pg) on respiratory antiviral immunity triggered by poly(I:C) was studied. Mice were nasally treated with viable (LV) or heat killed (LH) CRL1505 strain or Pg for 2d. Then, these groups and untreated control mice were nasally challenged with 3 daily doses of poly(I:C) (250 μg/mouse). INFγ, IFNβ, IFNα, TNFα, IL6, IL10 in bronchoalveolar lavage (BAL) and serum, expression of CD3, CD4, CD11b, CD11c, CD103, MHCII, IL10 and INFγ in lungs, and lung injury were determined. Poly(I:C) increased the levels of all the evaluated cytokines, accompanied by the recruitment of immune cells into the lung. In addition, increased protein content and LDH activity in BAL and altered lung wet weight:dry weight ratio were observed, indicating increased permeability of the alveolar-capillary barrier and lung injury. LV, LH or Pg administration enhanced INFγ, IFNβ and IL6 production and increased MHCII+CD103+, MHCII+CD11b+ dendritic cells and CD4+INFγ+and CD4+IL10+lymphocytes in lungs. Mice treated with LV, LH or Pg showed lower lung tissue damage. Results show that nasal administration of non-viable Lr1505 or its Pg maintains the probiotic properties of the viable strain and therefore they could be used to beneficially modulate the antiviral inflammatory response in the respiratory mucosa.
Fil: Kolling, Yanina Noralí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Zelaya, Hortensia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXX Annual Scientific Meeting Tucuman Biology Association
Horco Molle
Argentina
Asociación de Biología de Tucumán
description The effect of nasally administered Lactobacillus rhamnosus CRL1505 and its peptidoglycan (Pg) on respiratory antiviral immunity triggered by poly(I:C) was studied. Mice were nasally treated with viable (LV) or heat killed (LH) CRL1505 strain or Pg for 2d. Then, these groups and untreated control mice were nasally challenged with 3 daily doses of poly(I:C) (250 μg/mouse). INFγ, IFNβ, IFNα, TNFα, IL6, IL10 in bronchoalveolar lavage (BAL) and serum, expression of CD3, CD4, CD11b, CD11c, CD103, MHCII, IL10 and INFγ in lungs, and lung injury were determined. Poly(I:C) increased the levels of all the evaluated cytokines, accompanied by the recruitment of immune cells into the lung. In addition, increased protein content and LDH activity in BAL and altered lung wet weight:dry weight ratio were observed, indicating increased permeability of the alveolar-capillary barrier and lung injury. LV, LH or Pg administration enhanced INFγ, IFNβ and IL6 production and increased MHCII+CD103+, MHCII+CD11b+ dendritic cells and CD4+INFγ+and CD4+IL10+lymphocytes in lungs. Mice treated with LV, LH or Pg showed lower lung tissue damage. Results show that nasal administration of non-viable Lr1505 or its Pg maintains the probiotic properties of the viable strain and therefore they could be used to beneficially modulate the antiviral inflammatory response in the respiratory mucosa.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258240
Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity; XXX Annual Scientific Meeting Tucuman Biology Association; Horco Molle; Argentina; 2013; 42-42
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258240
identifier_str_mv Lactobacillus rhamnosus CRL1505 and its peptidoglycan as enhancers of antiviral respiratory immunity; XXX Annual Scientific Meeting Tucuman Biology Association; Horco Molle; Argentina; 2013; 42-42
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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