Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination

Autores
Sonzogni, Ana Sofía; Yealland, Guy; Kar, Mrityunjoy; Wedepohl, Stefanie; Gugliotta, Luis Marcelino; González, Verónica Doris Guadalupe; Hedtrich, Sarah; Calderon, Marcelo; Minari, Roque Javier
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Transdermal immunization is highly attractive because of the skin's accessibility and unique immunological characteristics. However, it remains a relatively unexplored route of administration because of the great difficulty of transporting antigens past the outermost layer of skin, the stratum corneum. In this article, the abilities of three poly(N-vinylcaprolactam) (PVCL)-based thermoresponsive assemblies - PVCL hydrogels and nanogels plus novel film forming PVCL/acrylic nanogels - to act as protein delivery systems were investigated. Similar thermal responses were observed in all systems, with transition temperatures close to 32 °C, close to that of the skin surface. The investigated dermal delivery systems showed no evidence of cytotoxicity in human fibroblasts and were able to load and release ovalbumin (OVA), a well-studied antigen, in a temperature-dependent manner in vitro. The penetration of OVA into ex vivo human skin following topical application was evaluated, where enhanced skin delivery was seen for the OVA-loaded PVCL systems relative to administration of the protein alone. The distinct protein release and skin penetration profiles observed for the different PVCL assemblies were here discussed on the basis of their structural differences.
Fil: Sonzogni, Ana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Yealland, Guy. Universidad Libre de Berlin; Alemania
Fil: Kar, Mrityunjoy. Universidad Libre de Berlin; Alemania
Fil: Wedepohl, Stefanie. Universidad Libre de Berlin; Alemania
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Hedtrich, Sarah. Universidad Libre de Berlin; Alemania
Fil: Calderon, Marcelo. Universidad Libre de Berlin; Alemania
Fil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
POLY(N-VINYLCAPROLAPTAM)
THERMORESPONSIVE POLYMERS
DERMAL DELIVERY SYSTEMS
PROTEIN SKIN PENETRATION
TRANSDERMAL IMMUNIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87040
Acceder
id CONICETDig_b39e2e9b94a885db9c242679d87cc292
oai_identifier_str oai:ri.conicet.gov.ar:11336/87040
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical VaccinationSonzogni, Ana SofíaYealland, GuyKar, MrityunjoyWedepohl, StefanieGugliotta, Luis MarcelinoGonzález, Verónica Doris GuadalupeHedtrich, SarahCalderon, MarceloMinari, Roque JavierPOLY(N-VINYLCAPROLAPTAM)THERMORESPONSIVE POLYMERSDERMAL DELIVERY SYSTEMSPROTEIN SKIN PENETRATIONTRANSDERMAL IMMUNIZATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Transdermal immunization is highly attractive because of the skin's accessibility and unique immunological characteristics. However, it remains a relatively unexplored route of administration because of the great difficulty of transporting antigens past the outermost layer of skin, the stratum corneum. In this article, the abilities of three poly(N-vinylcaprolactam) (PVCL)-based thermoresponsive assemblies - PVCL hydrogels and nanogels plus novel film forming PVCL/acrylic nanogels - to act as protein delivery systems were investigated. Similar thermal responses were observed in all systems, with transition temperatures close to 32 °C, close to that of the skin surface. The investigated dermal delivery systems showed no evidence of cytotoxicity in human fibroblasts and were able to load and release ovalbumin (OVA), a well-studied antigen, in a temperature-dependent manner in vitro. The penetration of OVA into ex vivo human skin following topical application was evaluated, where enhanced skin delivery was seen for the OVA-loaded PVCL systems relative to administration of the protein alone. The distinct protein release and skin penetration profiles observed for the different PVCL assemblies were here discussed on the basis of their structural differences.Fil: Sonzogni, Ana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Yealland, Guy. Universidad Libre de Berlin; AlemaniaFil: Kar, Mrityunjoy. Universidad Libre de Berlin; AlemaniaFil: Wedepohl, Stefanie. Universidad Libre de Berlin; AlemaniaFil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Hedtrich, Sarah. Universidad Libre de Berlin; AlemaniaFil: Calderon, Marcelo. Universidad Libre de Berlin; AlemaniaFil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaAmerican Chemical Society2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87040Sonzogni, Ana Sofía; Yealland, Guy; Kar, Mrityunjoy; Wedepohl, Stefanie; Gugliotta, Luis Marcelino; et al.; Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination; American Chemical Society; Biomacromolecules; 19; 12; 12-2018; 4607-46161525-7797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acs.biomac.8b01307info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.biomac.8b01307info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:08Zoai:ri.conicet.gov.ar:11336/87040instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:08.8CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
title Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
spellingShingle Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
Sonzogni, Ana Sofía
POLY(N-VINYLCAPROLAPTAM)
THERMORESPONSIVE POLYMERS
DERMAL DELIVERY SYSTEMS
PROTEIN SKIN PENETRATION
TRANSDERMAL IMMUNIZATION
title_short Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
title_full Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
title_fullStr Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
title_full_unstemmed Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
title_sort Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
dc.creator.none.fl_str_mv Sonzogni, Ana Sofía
Yealland, Guy
Kar, Mrityunjoy
Wedepohl, Stefanie
Gugliotta, Luis Marcelino
González, Verónica Doris Guadalupe
Hedtrich, Sarah
Calderon, Marcelo
Minari, Roque Javier
author Sonzogni, Ana Sofía
author_facet Sonzogni, Ana Sofía
Yealland, Guy
Kar, Mrityunjoy
Wedepohl, Stefanie
Gugliotta, Luis Marcelino
González, Verónica Doris Guadalupe
Hedtrich, Sarah
Calderon, Marcelo
Minari, Roque Javier
author_role author
author2 Yealland, Guy
Kar, Mrityunjoy
Wedepohl, Stefanie
Gugliotta, Luis Marcelino
González, Verónica Doris Guadalupe
Hedtrich, Sarah
Calderon, Marcelo
Minari, Roque Javier
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv POLY(N-VINYLCAPROLAPTAM)
THERMORESPONSIVE POLYMERS
DERMAL DELIVERY SYSTEMS
PROTEIN SKIN PENETRATION
TRANSDERMAL IMMUNIZATION
topic POLY(N-VINYLCAPROLAPTAM)
THERMORESPONSIVE POLYMERS
DERMAL DELIVERY SYSTEMS
PROTEIN SKIN PENETRATION
TRANSDERMAL IMMUNIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Transdermal immunization is highly attractive because of the skin's accessibility and unique immunological characteristics. However, it remains a relatively unexplored route of administration because of the great difficulty of transporting antigens past the outermost layer of skin, the stratum corneum. In this article, the abilities of three poly(N-vinylcaprolactam) (PVCL)-based thermoresponsive assemblies - PVCL hydrogels and nanogels plus novel film forming PVCL/acrylic nanogels - to act as protein delivery systems were investigated. Similar thermal responses were observed in all systems, with transition temperatures close to 32 °C, close to that of the skin surface. The investigated dermal delivery systems showed no evidence of cytotoxicity in human fibroblasts and were able to load and release ovalbumin (OVA), a well-studied antigen, in a temperature-dependent manner in vitro. The penetration of OVA into ex vivo human skin following topical application was evaluated, where enhanced skin delivery was seen for the OVA-loaded PVCL systems relative to administration of the protein alone. The distinct protein release and skin penetration profiles observed for the different PVCL assemblies were here discussed on the basis of their structural differences.
Fil: Sonzogni, Ana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Yealland, Guy. Universidad Libre de Berlin; Alemania
Fil: Kar, Mrityunjoy. Universidad Libre de Berlin; Alemania
Fil: Wedepohl, Stefanie. Universidad Libre de Berlin; Alemania
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Hedtrich, Sarah. Universidad Libre de Berlin; Alemania
Fil: Calderon, Marcelo. Universidad Libre de Berlin; Alemania
Fil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description Transdermal immunization is highly attractive because of the skin's accessibility and unique immunological characteristics. However, it remains a relatively unexplored route of administration because of the great difficulty of transporting antigens past the outermost layer of skin, the stratum corneum. In this article, the abilities of three poly(N-vinylcaprolactam) (PVCL)-based thermoresponsive assemblies - PVCL hydrogels and nanogels plus novel film forming PVCL/acrylic nanogels - to act as protein delivery systems were investigated. Similar thermal responses were observed in all systems, with transition temperatures close to 32 °C, close to that of the skin surface. The investigated dermal delivery systems showed no evidence of cytotoxicity in human fibroblasts and were able to load and release ovalbumin (OVA), a well-studied antigen, in a temperature-dependent manner in vitro. The penetration of OVA into ex vivo human skin following topical application was evaluated, where enhanced skin delivery was seen for the OVA-loaded PVCL systems relative to administration of the protein alone. The distinct protein release and skin penetration profiles observed for the different PVCL assemblies were here discussed on the basis of their structural differences.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87040
Sonzogni, Ana Sofía; Yealland, Guy; Kar, Mrityunjoy; Wedepohl, Stefanie; Gugliotta, Luis Marcelino; et al.; Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination; American Chemical Society; Biomacromolecules; 19; 12; 12-2018; 4607-4616
1525-7797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87040
identifier_str_mv Sonzogni, Ana Sofía; Yealland, Guy; Kar, Mrityunjoy; Wedepohl, Stefanie; Gugliotta, Luis Marcelino; et al.; Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination; American Chemical Society; Biomacromolecules; 19; 12; 12-2018; 4607-4616
1525-7797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acs.biomac.8b01307
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.biomac.8b01307
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082743198285824
score 13.221938

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