Effects of bile acid sequestration on hepatic steatosis in obese mice

Autores
Solís, Nancy; Pizarro, Margarita; Quintero, Pablo; Arab, Juan Pablo; Riquelme, Arnoldo; Padilla, Oslando; Carrasco, Gonzalo; Pirola, Carlos José; Sookoian, Silvia Cristina; Arrese, Marco
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated to type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyramine (CTM) administration on fatty liver development in the leptin-deficient obese mice. Aim: To study the effects of BAS on fatty liver development in obese mice. Methods: 4 week-old ob/ob mice (B6.V-Lepob/J, n=4-6 per group) were fed with CTM during 8 weeks. Serum and biliary parameters, glucose tolerance test (GTT), hepatic triglyceride content, liver histology and hepatic gene expression of relevant genes related to bile secretion, lipid and glucose metabolism were assessed. Results: 12-week-old mice exhibited marked obesity and hepatic steatosis. CTM administration expectedly determined a marked de-repression of 7-alpha-hydroxylase and decreased biliary bile acid secretion as well as improved GTT. CTM feeding showed no effects on hepatic triglyceride content or in the degree of steatosis on liver histology. CTM was associated to increased levels of serum alanine-aminotransferase. Conclusion: Although CTM administration positively affects glucose tolerance it does not prevent hepatic steatosis development in obese mice. Moreover, CTM feeding was associated to liver enzyme elevation in this model of NAFLD. Thus, the effects BAS on NAFLD need to be specifically addressed since this therapy might not be beneficial for NAFLD.
Fil: Solís, Nancy. Pontificia Universidad Católica de Chile; Chile
Fil: Pizarro, Margarita. Pontificia Universidad Católica de Chile; Chile
Fil: Quintero, Pablo. Pontificia Universidad Católica de Chile; Chile
Fil: Arab, Juan Pablo. Pontificia Universidad Católica de Chile; Chile
Fil: Riquelme, Arnoldo. Pontificia Universidad Católica de Chile; Chile
Fil: Padilla, Oslando. Pontificia Universidad Católica de Chile; Chile
Fil: Carrasco, Gonzalo. Hospital Parroquial de San Bernardo; Chile
Fil: Pirola, Carlos José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Sookoian, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Arrese, Marco. Pontificia Universidad Católica de Chile; Chile
Materia
BILE ACIDS
FATTY LIVER
OB MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84831
Acceder
id CONICETDig_b36761115ffbbeb0c634e9ee33d794a7
oai_identifier_str oai:ri.conicet.gov.ar:11336/84831
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of bile acid sequestration on hepatic steatosis in obese miceSolís, NancyPizarro, MargaritaQuintero, PabloArab, Juan PabloRiquelme, ArnoldoPadilla, OslandoCarrasco, GonzaloPirola, Carlos JoséSookoian, Silvia CristinaArrese, MarcoBILE ACIDSFATTY LIVEROB MICEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated to type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyramine (CTM) administration on fatty liver development in the leptin-deficient obese mice. Aim: To study the effects of BAS on fatty liver development in obese mice. Methods: 4 week-old ob/ob mice (B6.V-Lepob/J, n=4-6 per group) were fed with CTM during 8 weeks. Serum and biliary parameters, glucose tolerance test (GTT), hepatic triglyceride content, liver histology and hepatic gene expression of relevant genes related to bile secretion, lipid and glucose metabolism were assessed. Results: 12-week-old mice exhibited marked obesity and hepatic steatosis. CTM administration expectedly determined a marked de-repression of 7-alpha-hydroxylase and decreased biliary bile acid secretion as well as improved GTT. CTM feeding showed no effects on hepatic triglyceride content or in the degree of steatosis on liver histology. CTM was associated to increased levels of serum alanine-aminotransferase. Conclusion: Although CTM administration positively affects glucose tolerance it does not prevent hepatic steatosis development in obese mice. Moreover, CTM feeding was associated to liver enzyme elevation in this model of NAFLD. Thus, the effects BAS on NAFLD need to be specifically addressed since this therapy might not be beneficial for NAFLD.Fil: Solís, Nancy. Pontificia Universidad Católica de Chile; ChileFil: Pizarro, Margarita. Pontificia Universidad Católica de Chile; ChileFil: Quintero, Pablo. Pontificia Universidad Católica de Chile; ChileFil: Arab, Juan Pablo. Pontificia Universidad Católica de Chile; ChileFil: Riquelme, Arnoldo. Pontificia Universidad Católica de Chile; ChileFil: Padilla, Oslando. Pontificia Universidad Católica de Chile; ChileFil: Carrasco, Gonzalo. Hospital Parroquial de San Bernardo; ChileFil: Pirola, Carlos José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Sookoian, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Arrese, Marco. Pontificia Universidad Católica de Chile; ChileMexican Association of Hepatology2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84831Solís, Nancy; Pizarro, Margarita; Quintero, Pablo; Arab, Juan Pablo; Riquelme, Arnoldo; et al.; Effects of bile acid sequestration on hepatic steatosis in obese mice; Mexican Association of Hepatology; Annals of Hepatology; 13; 1; 1-2014; 105-1121665-2681CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S1665-2681(19)30911-1info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1665268119309111info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:17Zoai:ri.conicet.gov.ar:11336/84831instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:17.27CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of bile acid sequestration on hepatic steatosis in obese mice
title Effects of bile acid sequestration on hepatic steatosis in obese mice
spellingShingle Effects of bile acid sequestration on hepatic steatosis in obese mice
Solís, Nancy
BILE ACIDS
FATTY LIVER
OB MICE
title_short Effects of bile acid sequestration on hepatic steatosis in obese mice
title_full Effects of bile acid sequestration on hepatic steatosis in obese mice
title_fullStr Effects of bile acid sequestration on hepatic steatosis in obese mice
title_full_unstemmed Effects of bile acid sequestration on hepatic steatosis in obese mice
title_sort Effects of bile acid sequestration on hepatic steatosis in obese mice
dc.creator.none.fl_str_mv Solís, Nancy
Pizarro, Margarita
Quintero, Pablo
Arab, Juan Pablo
Riquelme, Arnoldo
Padilla, Oslando
Carrasco, Gonzalo
Pirola, Carlos José
Sookoian, Silvia Cristina
Arrese, Marco
author Solís, Nancy
author_facet Solís, Nancy
Pizarro, Margarita
Quintero, Pablo
Arab, Juan Pablo
Riquelme, Arnoldo
Padilla, Oslando
Carrasco, Gonzalo
Pirola, Carlos José
Sookoian, Silvia Cristina
Arrese, Marco
author_role author
author2 Pizarro, Margarita
Quintero, Pablo
Arab, Juan Pablo
Riquelme, Arnoldo
Padilla, Oslando
Carrasco, Gonzalo
Pirola, Carlos José
Sookoian, Silvia Cristina
Arrese, Marco
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BILE ACIDS
FATTY LIVER
OB MICE
topic BILE ACIDS
FATTY LIVER
OB MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated to type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyramine (CTM) administration on fatty liver development in the leptin-deficient obese mice. Aim: To study the effects of BAS on fatty liver development in obese mice. Methods: 4 week-old ob/ob mice (B6.V-Lepob/J, n=4-6 per group) were fed with CTM during 8 weeks. Serum and biliary parameters, glucose tolerance test (GTT), hepatic triglyceride content, liver histology and hepatic gene expression of relevant genes related to bile secretion, lipid and glucose metabolism were assessed. Results: 12-week-old mice exhibited marked obesity and hepatic steatosis. CTM administration expectedly determined a marked de-repression of 7-alpha-hydroxylase and decreased biliary bile acid secretion as well as improved GTT. CTM feeding showed no effects on hepatic triglyceride content or in the degree of steatosis on liver histology. CTM was associated to increased levels of serum alanine-aminotransferase. Conclusion: Although CTM administration positively affects glucose tolerance it does not prevent hepatic steatosis development in obese mice. Moreover, CTM feeding was associated to liver enzyme elevation in this model of NAFLD. Thus, the effects BAS on NAFLD need to be specifically addressed since this therapy might not be beneficial for NAFLD.
Fil: Solís, Nancy. Pontificia Universidad Católica de Chile; Chile
Fil: Pizarro, Margarita. Pontificia Universidad Católica de Chile; Chile
Fil: Quintero, Pablo. Pontificia Universidad Católica de Chile; Chile
Fil: Arab, Juan Pablo. Pontificia Universidad Católica de Chile; Chile
Fil: Riquelme, Arnoldo. Pontificia Universidad Católica de Chile; Chile
Fil: Padilla, Oslando. Pontificia Universidad Católica de Chile; Chile
Fil: Carrasco, Gonzalo. Hospital Parroquial de San Bernardo; Chile
Fil: Pirola, Carlos José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Sookoian, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Arrese, Marco. Pontificia Universidad Católica de Chile; Chile
description Background: Bile acid sequestration (BAS) with resins has shown antidiabetic effects in both humans and animals. Since hepatic steatosis is commonly associated to type 2 diabetes mellitus and the effects of BAS on steatosis have not been explored in detail, we evaluated the effects of cholestyramine (CTM) administration on fatty liver development in the leptin-deficient obese mice. Aim: To study the effects of BAS on fatty liver development in obese mice. Methods: 4 week-old ob/ob mice (B6.V-Lepob/J, n=4-6 per group) were fed with CTM during 8 weeks. Serum and biliary parameters, glucose tolerance test (GTT), hepatic triglyceride content, liver histology and hepatic gene expression of relevant genes related to bile secretion, lipid and glucose metabolism were assessed. Results: 12-week-old mice exhibited marked obesity and hepatic steatosis. CTM administration expectedly determined a marked de-repression of 7-alpha-hydroxylase and decreased biliary bile acid secretion as well as improved GTT. CTM feeding showed no effects on hepatic triglyceride content or in the degree of steatosis on liver histology. CTM was associated to increased levels of serum alanine-aminotransferase. Conclusion: Although CTM administration positively affects glucose tolerance it does not prevent hepatic steatosis development in obese mice. Moreover, CTM feeding was associated to liver enzyme elevation in this model of NAFLD. Thus, the effects BAS on NAFLD need to be specifically addressed since this therapy might not be beneficial for NAFLD.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84831
Solís, Nancy; Pizarro, Margarita; Quintero, Pablo; Arab, Juan Pablo; Riquelme, Arnoldo; et al.; Effects of bile acid sequestration on hepatic steatosis in obese mice; Mexican Association of Hepatology; Annals of Hepatology; 13; 1; 1-2014; 105-112
1665-2681
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84831
identifier_str_mv Solís, Nancy; Pizarro, Margarita; Quintero, Pablo; Arab, Juan Pablo; Riquelme, Arnoldo; et al.; Effects of bile acid sequestration on hepatic steatosis in obese mice; Mexican Association of Hepatology; Annals of Hepatology; 13; 1; 1-2014; 105-112
1665-2681
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S1665-2681(19)30911-1
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1665268119309111
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mexican Association of Hepatology
publisher.none.fl_str_mv Mexican Association of Hepatology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980006263783424
score 12.993085

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