A simple method to obtain endophytic microorganisms from field-collected roots

Autores
Silvani, Vanesa Analia; Fracchia, S.; Fernández, L.; Pérgola, M.; Godeas, Alicia Margarita
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar(TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space.
Fil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Fracchia, S.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Fernández, L.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Pérgola, M.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Materia
AMF
BACTERIA
DSE
FIELD ROOTS
IN VITRO CULTURE
ROOT ENDOPHYTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163615
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A simple method to obtain endophytic microorganisms from field-collected rootsSilvani, Vanesa AnaliaFracchia, S.Fernández, L.Pérgola, M.Godeas, Alicia MargaritaAMFBACTERIADSEFIELD ROOTSIN VITRO CULTUREROOT ENDOPHYTEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar(TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space.Fil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Fracchia, S.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Fernández, L.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Pérgola, M.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaPergamon-Elsevier Science Ltd2008-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163615Silvani, Vanesa Analia; Fracchia, S.; Fernández, L.; Pérgola, M.; Godeas, Alicia Margarita; A simple method to obtain endophytic microorganisms from field-collected roots; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 40; 5; 5-2008; 1259-12630038-0717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0038071707004695info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2007.11.022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:50Zoai:ri.conicet.gov.ar:11336/163615instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:50.646CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simple method to obtain endophytic microorganisms from field-collected roots
title A simple method to obtain endophytic microorganisms from field-collected roots
spellingShingle A simple method to obtain endophytic microorganisms from field-collected roots
Silvani, Vanesa Analia
AMF
BACTERIA
DSE
FIELD ROOTS
IN VITRO CULTURE
ROOT ENDOPHYTES
title_short A simple method to obtain endophytic microorganisms from field-collected roots
title_full A simple method to obtain endophytic microorganisms from field-collected roots
title_fullStr A simple method to obtain endophytic microorganisms from field-collected roots
title_full_unstemmed A simple method to obtain endophytic microorganisms from field-collected roots
title_sort A simple method to obtain endophytic microorganisms from field-collected roots
dc.creator.none.fl_str_mv Silvani, Vanesa Analia
Fracchia, S.
Fernández, L.
Pérgola, M.
Godeas, Alicia Margarita
author Silvani, Vanesa Analia
author_facet Silvani, Vanesa Analia
Fracchia, S.
Fernández, L.
Pérgola, M.
Godeas, Alicia Margarita
author_role author
author2 Fracchia, S.
Fernández, L.
Pérgola, M.
Godeas, Alicia Margarita
author2_role author
author
author
author
dc.subject.none.fl_str_mv AMF
BACTERIA
DSE
FIELD ROOTS
IN VITRO CULTURE
ROOT ENDOPHYTES
topic AMF
BACTERIA
DSE
FIELD ROOTS
IN VITRO CULTURE
ROOT ENDOPHYTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar(TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space.
Fil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Fracchia, S.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Fernández, L.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Pérgola, M.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
description We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar(TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space.
publishDate 2008
dc.date.none.fl_str_mv 2008-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163615
Silvani, Vanesa Analia; Fracchia, S.; Fernández, L.; Pérgola, M.; Godeas, Alicia Margarita; A simple method to obtain endophytic microorganisms from field-collected roots; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 40; 5; 5-2008; 1259-1263
0038-0717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163615
identifier_str_mv Silvani, Vanesa Analia; Fracchia, S.; Fernández, L.; Pérgola, M.; Godeas, Alicia Margarita; A simple method to obtain endophytic microorganisms from field-collected roots; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 40; 5; 5-2008; 1259-1263
0038-0717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0038071707004695
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2007.11.022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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