Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex

Autores
Agüero, Tristán Horacio; Jin, Zhigang; Chorghade, Sandip; Kalsotra, Auinash; King, Mary Lou; Yang, Jing
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.
Fil: Agüero, Tristán Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Miami; Estados Unidos
Fil: Jin, Zhigang. University of Illinois at Urbana; Estados Unidos
Fil: Chorghade, Sandip. University of Illinois at Urbana; Estados Unidos
Fil: Kalsotra, Auinash. University of Illinois at Urbana; Estados Unidos
Fil: King, Mary Lou. University of Miami; Estados Unidos
Fil: Yang, Jing. University of Illinois at Urbana; Estados Unidos
Materia
DND1
GERMLINE DEVELOPMENT
NANOS
TRANSLATION REGULATION
XENOPUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53903
Acceder
id CONICETDig_b253d0e039acc7b44839b174a5f3e659
oai_identifier_str oai:ri.conicet.gov.ar:11336/53903
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complexAgüero, Tristán HoracioJin, ZhigangChorghade, SandipKalsotra, AuinashKing, Mary LouYang, JingDND1GERMLINE DEVELOPMENTNANOSTRANSLATION REGULATIONXENOPUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.Fil: Agüero, Tristán Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Miami; Estados UnidosFil: Jin, Zhigang. University of Illinois at Urbana; Estados UnidosFil: Chorghade, Sandip. University of Illinois at Urbana; Estados UnidosFil: Kalsotra, Auinash. University of Illinois at Urbana; Estados UnidosFil: King, Mary Lou. University of Miami; Estados UnidosFil: Yang, Jing. University of Illinois at Urbana; Estados UnidosCompany of Biologists2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53903Agüero, Tristán Horacio; Jin, Zhigang; Chorghade, Sandip; Kalsotra, Auinash; King, Mary Lou; et al.; Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex; Company of Biologists; Development; 144; 10-2017; 3755-37750950-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/content/144/20/3755info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.152611info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:29Zoai:ri.conicet.gov.ar:11336/53903instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:29.393CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
title Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
spellingShingle Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
Agüero, Tristán Horacio
DND1
GERMLINE DEVELOPMENT
NANOS
TRANSLATION REGULATION
XENOPUS
title_short Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
title_full Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
title_fullStr Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
title_full_unstemmed Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
title_sort Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex
dc.creator.none.fl_str_mv Agüero, Tristán Horacio
Jin, Zhigang
Chorghade, Sandip
Kalsotra, Auinash
King, Mary Lou
Yang, Jing
author Agüero, Tristán Horacio
author_facet Agüero, Tristán Horacio
Jin, Zhigang
Chorghade, Sandip
Kalsotra, Auinash
King, Mary Lou
Yang, Jing
author_role author
author2 Jin, Zhigang
Chorghade, Sandip
Kalsotra, Auinash
King, Mary Lou
Yang, Jing
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DND1
GERMLINE DEVELOPMENT
NANOS
TRANSLATION REGULATION
XENOPUS
topic DND1
GERMLINE DEVELOPMENT
NANOS
TRANSLATION REGULATION
XENOPUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.
Fil: Agüero, Tristán Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Miami; Estados Unidos
Fil: Jin, Zhigang. University of Illinois at Urbana; Estados Unidos
Fil: Chorghade, Sandip. University of Illinois at Urbana; Estados Unidos
Fil: Kalsotra, Auinash. University of Illinois at Urbana; Estados Unidos
Fil: King, Mary Lou. University of Miami; Estados Unidos
Fil: Yang, Jing. University of Illinois at Urbana; Estados Unidos
description In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53903
Agüero, Tristán Horacio; Jin, Zhigang; Chorghade, Sandip; Kalsotra, Auinash; King, Mary Lou; et al.; Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex; Company of Biologists; Development; 144; 10-2017; 3755-3775
0950-1991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53903
identifier_str_mv Agüero, Tristán Horacio; Jin, Zhigang; Chorghade, Sandip; Kalsotra, Auinash; King, Mary Lou; et al.; Maternal dead-end 1 promotes translation of nanos1 by binding the eiF3 complex; Company of Biologists; Development; 144; 10-2017; 3755-3775
0950-1991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/content/144/20/3755
info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.152611
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614276822597632
score 13.070432

Publicaciones similares