Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis

Autores
Agüero, Tristán Horacio; Zhou, Yi; Kloc, Malgorzata; Chang, Patrick; Houliston, Evelyn; King, Mary Lou
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The germ cell lineage in Xenopus is specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such as nanos1, localize to the germ plasm. nanos1 has the essential germline function of blocking somatic gene expression and thus preventing Primordial Germ Cell (PGC) loss and sterility. Hermes/Rbpms protein and nanos RNA co-localize within germinal granules, diagnostic electron dense particles found within the germ plasm. Previous work indicates that nanos accumulates within the germ plasm through a diffusion/entrapment mechanism. Here we show that Hermes/Rbpms interacts with nanos through sequence specific RNA localization signals found in the nanos 31UTR. Importantly, Hermes/Rbpms specifically binds nanos, but not Vg1 RNA in the nucleus of stage I oocytes. In vitro binding data show that Hermes/Rbpms requires additional factors that are present in stage I oocytes in order to bind nanos1. One such factor may be hnRNP I, identified in a yeast-2-hybrid screen as directly interacting with Hermes/Rbpms. We suggest that Hermes/Rbpms functions as part of a RNP complex in the nucleus that facilitates selection of germline RNAs for germ plasm localization. We propose that Hermes/Rbpms is required for nanos RNA to form within the germinal granules and in this way, participates in the germline specific translational repression and sequestration of nanos RNA.
Fil: Agüero, Tristán Horacio. University of Miami; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zhou, Yi. University of Miami; Estados Unidos
Fil: Kloc, Malgorzata. The Houston Methodist Hospital; Estados Unidos
Fil: Chang, Patrick. Sorbonne University.; Francia
Fil: Houliston, Evelyn. Sorbonne University.; Francia
Fil: King, Mary Lou. University of Miami; Estados Unidos
Materia
XENOPUS OOGENESIS
NANOS1 RNA LOCALIZATION
GERMLINE
RNP PARTICLE
GERMLINE GRANULES
NANOS RIBONUCLEOPROTEIN COMPLEX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101694
Acceder
id CONICETDig_7b1e2bb7ef60c387d828c81c1d2abca6
oai_identifier_str oai:ri.conicet.gov.ar:11336/101694
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesisAgüero, Tristán HoracioZhou, YiKloc, MalgorzataChang, PatrickHouliston, EvelynKing, Mary LouXENOPUS OOGENESISNANOS1 RNA LOCALIZATIONGERMLINERNP PARTICLEGERMLINE GRANULESNANOS RIBONUCLEOPROTEIN COMPLEXhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The germ cell lineage in Xenopus is specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such as nanos1, localize to the germ plasm. nanos1 has the essential germline function of blocking somatic gene expression and thus preventing Primordial Germ Cell (PGC) loss and sterility. Hermes/Rbpms protein and nanos RNA co-localize within germinal granules, diagnostic electron dense particles found within the germ plasm. Previous work indicates that nanos accumulates within the germ plasm through a diffusion/entrapment mechanism. Here we show that Hermes/Rbpms interacts with nanos through sequence specific RNA localization signals found in the nanos 31UTR. Importantly, Hermes/Rbpms specifically binds nanos, but not Vg1 RNA in the nucleus of stage I oocytes. In vitro binding data show that Hermes/Rbpms requires additional factors that are present in stage I oocytes in order to bind nanos1. One such factor may be hnRNP I, identified in a yeast-2-hybrid screen as directly interacting with Hermes/Rbpms. We suggest that Hermes/Rbpms functions as part of a RNP complex in the nucleus that facilitates selection of germline RNAs for germ plasm localization. We propose that Hermes/Rbpms is required for nanos RNA to form within the germinal granules and in this way, participates in the germline specific translational repression and sequestration of nanos RNA.Fil: Agüero, Tristán Horacio. University of Miami; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Zhou, Yi. University of Miami; Estados UnidosFil: Kloc, Malgorzata. The Houston Methodist Hospital; Estados UnidosFil: Chang, Patrick. Sorbonne University.; FranciaFil: Houliston, Evelyn. Sorbonne University.; FranciaFil: King, Mary Lou. University of Miami; Estados UnidosMDPI2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101694Agüero, Tristán Horacio; Zhou, Yi; Kloc, Malgorzata; Chang, Patrick; Houliston, Evelyn; et al.; Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis; MDPI; Journal of Developmental Biology; 4; 1; 3-2016; 1-172221-3759CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/jdb4010002info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2221-3759/4/1/2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:01Zoai:ri.conicet.gov.ar:11336/101694instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:01.255CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
title Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
spellingShingle Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
Agüero, Tristán Horacio
XENOPUS OOGENESIS
NANOS1 RNA LOCALIZATION
GERMLINE
RNP PARTICLE
GERMLINE GRANULES
NANOS RIBONUCLEOPROTEIN COMPLEX
title_short Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
title_full Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
title_fullStr Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
title_full_unstemmed Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
title_sort Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis
dc.creator.none.fl_str_mv Agüero, Tristán Horacio
Zhou, Yi
Kloc, Malgorzata
Chang, Patrick
Houliston, Evelyn
King, Mary Lou
author Agüero, Tristán Horacio
author_facet Agüero, Tristán Horacio
Zhou, Yi
Kloc, Malgorzata
Chang, Patrick
Houliston, Evelyn
King, Mary Lou
author_role author
author2 Zhou, Yi
Kloc, Malgorzata
Chang, Patrick
Houliston, Evelyn
King, Mary Lou
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv XENOPUS OOGENESIS
NANOS1 RNA LOCALIZATION
GERMLINE
RNP PARTICLE
GERMLINE GRANULES
NANOS RIBONUCLEOPROTEIN COMPLEX
topic XENOPUS OOGENESIS
NANOS1 RNA LOCALIZATION
GERMLINE
RNP PARTICLE
GERMLINE GRANULES
NANOS RIBONUCLEOPROTEIN COMPLEX
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The germ cell lineage in Xenopus is specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such as nanos1, localize to the germ plasm. nanos1 has the essential germline function of blocking somatic gene expression and thus preventing Primordial Germ Cell (PGC) loss and sterility. Hermes/Rbpms protein and nanos RNA co-localize within germinal granules, diagnostic electron dense particles found within the germ plasm. Previous work indicates that nanos accumulates within the germ plasm through a diffusion/entrapment mechanism. Here we show that Hermes/Rbpms interacts with nanos through sequence specific RNA localization signals found in the nanos 31UTR. Importantly, Hermes/Rbpms specifically binds nanos, but not Vg1 RNA in the nucleus of stage I oocytes. In vitro binding data show that Hermes/Rbpms requires additional factors that are present in stage I oocytes in order to bind nanos1. One such factor may be hnRNP I, identified in a yeast-2-hybrid screen as directly interacting with Hermes/Rbpms. We suggest that Hermes/Rbpms functions as part of a RNP complex in the nucleus that facilitates selection of germline RNAs for germ plasm localization. We propose that Hermes/Rbpms is required for nanos RNA to form within the germinal granules and in this way, participates in the germline specific translational repression and sequestration of nanos RNA.
Fil: Agüero, Tristán Horacio. University of Miami; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zhou, Yi. University of Miami; Estados Unidos
Fil: Kloc, Malgorzata. The Houston Methodist Hospital; Estados Unidos
Fil: Chang, Patrick. Sorbonne University.; Francia
Fil: Houliston, Evelyn. Sorbonne University.; Francia
Fil: King, Mary Lou. University of Miami; Estados Unidos
description The germ cell lineage in Xenopus is specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such as nanos1, localize to the germ plasm. nanos1 has the essential germline function of blocking somatic gene expression and thus preventing Primordial Germ Cell (PGC) loss and sterility. Hermes/Rbpms protein and nanos RNA co-localize within germinal granules, diagnostic electron dense particles found within the germ plasm. Previous work indicates that nanos accumulates within the germ plasm through a diffusion/entrapment mechanism. Here we show that Hermes/Rbpms interacts with nanos through sequence specific RNA localization signals found in the nanos 31UTR. Importantly, Hermes/Rbpms specifically binds nanos, but not Vg1 RNA in the nucleus of stage I oocytes. In vitro binding data show that Hermes/Rbpms requires additional factors that are present in stage I oocytes in order to bind nanos1. One such factor may be hnRNP I, identified in a yeast-2-hybrid screen as directly interacting with Hermes/Rbpms. We suggest that Hermes/Rbpms functions as part of a RNP complex in the nucleus that facilitates selection of germline RNAs for germ plasm localization. We propose that Hermes/Rbpms is required for nanos RNA to form within the germinal granules and in this way, participates in the germline specific translational repression and sequestration of nanos RNA.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101694
Agüero, Tristán Horacio; Zhou, Yi; Kloc, Malgorzata; Chang, Patrick; Houliston, Evelyn; et al.; Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis; MDPI; Journal of Developmental Biology; 4; 1; 3-2016; 1-17
2221-3759
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101694
identifier_str_mv Agüero, Tristán Horacio; Zhou, Yi; Kloc, Malgorzata; Chang, Patrick; Houliston, Evelyn; et al.; Hermes (Rbpms) is a critical component of RNP complexes that sequester germline RNAs during oogenesis; MDPI; Journal of Developmental Biology; 4; 1; 3-2016; 1-17
2221-3759
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/jdb4010002
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2221-3759/4/1/2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613164460670976
score 13.070432

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