Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)

Autores
Laurito, Magdalena; Ontivero, Iliana Mayra; Almiron, Walter Ricardo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sand f ly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand f lies of the New World.
Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina
Fil: Ontivero, Iliana Mayra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina
Fil: Almiron, Walter Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
DNA BARCODING
EVANDROMYIA CORTELEZZII
MIGONEMYIA MIGONEI
SPECIES DELIMITATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96692
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)Laurito, MagdalenaOntivero, Iliana MayraAlmiron, Walter RicardoDNA BARCODINGEVANDROMYIA CORTELEZZIIMIGONEMYIA MIGONEISPECIES DELIMITATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sand f ly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand f lies of the New World.Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; ArgentinaFil: Ontivero, Iliana Mayra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; ArgentinaFil: Almiron, Walter Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFundação Oswaldo Cruz2019-11-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96692Laurito, Magdalena; Ontivero, Iliana Mayra; Almiron, Walter Ricardo; Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae); Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 11; 28-11-20190074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760190208info:eu-repo/semantics/altIdentifier/url/https://memorias.ioc.fiocruz.br/article/9993/0208-increasing-the-digital-repository-of-dna-barcoding-sequences-of-sand-flies-psychodidae-phlebotominaeinfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/ktqt6cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:06Zoai:ri.conicet.gov.ar:11336/96692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:06.966CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
title Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
spellingShingle Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
Laurito, Magdalena
DNA BARCODING
EVANDROMYIA CORTELEZZII
MIGONEMYIA MIGONEI
SPECIES DELIMITATION
title_short Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
title_full Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
title_fullStr Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
title_full_unstemmed Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
title_sort Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
dc.creator.none.fl_str_mv Laurito, Magdalena
Ontivero, Iliana Mayra
Almiron, Walter Ricardo
author Laurito, Magdalena
author_facet Laurito, Magdalena
Ontivero, Iliana Mayra
Almiron, Walter Ricardo
author_role author
author2 Ontivero, Iliana Mayra
Almiron, Walter Ricardo
author2_role author
author
dc.subject.none.fl_str_mv DNA BARCODING
EVANDROMYIA CORTELEZZII
MIGONEMYIA MIGONEI
SPECIES DELIMITATION
topic DNA BARCODING
EVANDROMYIA CORTELEZZII
MIGONEMYIA MIGONEI
SPECIES DELIMITATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sand f ly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand f lies of the New World.
Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina
Fil: Ontivero, Iliana Mayra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina
Fil: Almiron, Walter Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description Sand f ly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand f lies of the New World.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96692
Laurito, Magdalena; Ontivero, Iliana Mayra; Almiron, Walter Ricardo; Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae); Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 11; 28-11-2019
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96692
identifier_str_mv Laurito, Magdalena; Ontivero, Iliana Mayra; Almiron, Walter Ricardo; Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae); Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 11; 28-11-2019
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760190208
info:eu-repo/semantics/altIdentifier/url/https://memorias.ioc.fiocruz.br/article/9993/0208-increasing-the-digital-repository-of-dna-barcoding-sequences-of-sand-flies-psychodidae-phlebotominae
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/ktqt6c
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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