Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy

Autores
Argañaraz, Martin Eduardo; Apichela, Silvana Andrea; Zampini, Renato; Vencato, Juri; Stelletta, Calogero
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
South American camelids show high embryo loss rate, during the first 60 days of pregnancy. One of the factors which may be related to this situation is that over 98% of the embryos implant in the left uterine horn (LUH) even though both ovaries contribute similarly to ovulation. There is scarce information about the uterine environment of female camelids at any physiological state that could explain the capability of the LUH to attract the embryo and maintain pregnancy. We describe, for the first time, the biochemical and protein profile of uterine fluid (UF), addressing the right and LUH environment in non-pregnant and pregnant alpacas. Different substrates, electrolytes and metabolites were assayed in both uterine horn fluids. Small changes were observed in glucose and total protein levels, which were more noticeable during pregnancy. In addition, 10 specific proteins were found in the left horn fluid in 5-week-pregnant alpacas, and two protein bands were identified in non-pregnant alpaca right horn fluid. These results would provide basic information for identification of possible markers for pregnancy diagnosis, reproductive diseases and hormone-treated animals evaluation and hence contributing to improve the pregnancy rate.
Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Cátedra de Zootecnia General I; Argentina
Fil: Zampini, Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vencato, Juri. Università di Padova; Italia
Fil: Stelletta, Calogero. Università di Padova; Italia
Materia
ALPACA
UTERUS
PREGNANCY
UTERINE FLUID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151825
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151825
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancyArgañaraz, Martin EduardoApichela, Silvana AndreaZampini, RenatoVencato, JuriStelletta, CalogeroALPACAUTERUSPREGNANCYUTERINE FLUIDhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4South American camelids show high embryo loss rate, during the first 60 days of pregnancy. One of the factors which may be related to this situation is that over 98% of the embryos implant in the left uterine horn (LUH) even though both ovaries contribute similarly to ovulation. There is scarce information about the uterine environment of female camelids at any physiological state that could explain the capability of the LUH to attract the embryo and maintain pregnancy. We describe, for the first time, the biochemical and protein profile of uterine fluid (UF), addressing the right and LUH environment in non-pregnant and pregnant alpacas. Different substrates, electrolytes and metabolites were assayed in both uterine horn fluids. Small changes were observed in glucose and total protein levels, which were more noticeable during pregnancy. In addition, 10 specific proteins were found in the left horn fluid in 5-week-pregnant alpacas, and two protein bands were identified in non-pregnant alpaca right horn fluid. These results would provide basic information for identification of possible markers for pregnancy diagnosis, reproductive diseases and hormone-treated animals evaluation and hence contributing to improve the pregnancy rate.Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Cátedra de Zootecnia General I; ArgentinaFil: Zampini, Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vencato, Juri. Università di Padova; ItaliaFil: Stelletta, Calogero. Università di Padova; ItaliaWiley Blackwell Publishing, Inc2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151825Argañaraz, Martin Eduardo; Apichela, Silvana Andrea; Zampini, Renato; Vencato, Juri; Stelletta, Calogero; Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 1; 2-2015; 121-1280936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12460info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/rda.12460info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:43Zoai:ri.conicet.gov.ar:11336/151825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:43.492CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
title Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
spellingShingle Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
Argañaraz, Martin Eduardo
ALPACA
UTERUS
PREGNANCY
UTERINE FLUID
title_short Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
title_full Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
title_fullStr Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
title_full_unstemmed Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
title_sort Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy
dc.creator.none.fl_str_mv Argañaraz, Martin Eduardo
Apichela, Silvana Andrea
Zampini, Renato
Vencato, Juri
Stelletta, Calogero
author Argañaraz, Martin Eduardo
author_facet Argañaraz, Martin Eduardo
Apichela, Silvana Andrea
Zampini, Renato
Vencato, Juri
Stelletta, Calogero
author_role author
author2 Apichela, Silvana Andrea
Zampini, Renato
Vencato, Juri
Stelletta, Calogero
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALPACA
UTERUS
PREGNANCY
UTERINE FLUID
topic ALPACA
UTERUS
PREGNANCY
UTERINE FLUID
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv South American camelids show high embryo loss rate, during the first 60 days of pregnancy. One of the factors which may be related to this situation is that over 98% of the embryos implant in the left uterine horn (LUH) even though both ovaries contribute similarly to ovulation. There is scarce information about the uterine environment of female camelids at any physiological state that could explain the capability of the LUH to attract the embryo and maintain pregnancy. We describe, for the first time, the biochemical and protein profile of uterine fluid (UF), addressing the right and LUH environment in non-pregnant and pregnant alpacas. Different substrates, electrolytes and metabolites were assayed in both uterine horn fluids. Small changes were observed in glucose and total protein levels, which were more noticeable during pregnancy. In addition, 10 specific proteins were found in the left horn fluid in 5-week-pregnant alpacas, and two protein bands were identified in non-pregnant alpaca right horn fluid. These results would provide basic information for identification of possible markers for pregnancy diagnosis, reproductive diseases and hormone-treated animals evaluation and hence contributing to improve the pregnancy rate.
Fil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Cátedra de Zootecnia General I; Argentina
Fil: Zampini, Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vencato, Juri. Università di Padova; Italia
Fil: Stelletta, Calogero. Università di Padova; Italia
description South American camelids show high embryo loss rate, during the first 60 days of pregnancy. One of the factors which may be related to this situation is that over 98% of the embryos implant in the left uterine horn (LUH) even though both ovaries contribute similarly to ovulation. There is scarce information about the uterine environment of female camelids at any physiological state that could explain the capability of the LUH to attract the embryo and maintain pregnancy. We describe, for the first time, the biochemical and protein profile of uterine fluid (UF), addressing the right and LUH environment in non-pregnant and pregnant alpacas. Different substrates, electrolytes and metabolites were assayed in both uterine horn fluids. Small changes were observed in glucose and total protein levels, which were more noticeable during pregnancy. In addition, 10 specific proteins were found in the left horn fluid in 5-week-pregnant alpacas, and two protein bands were identified in non-pregnant alpaca right horn fluid. These results would provide basic information for identification of possible markers for pregnancy diagnosis, reproductive diseases and hormone-treated animals evaluation and hence contributing to improve the pregnancy rate.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151825
Argañaraz, Martin Eduardo; Apichela, Silvana Andrea; Zampini, Renato; Vencato, Juri; Stelletta, Calogero; Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 1; 2-2015; 121-128
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151825
identifier_str_mv Argañaraz, Martin Eduardo; Apichela, Silvana Andrea; Zampini, Renato; Vencato, Juri; Stelletta, Calogero; Biochemical and protein profile of alpaca (vicugna pacos) uterine horn fluid during early pregnancy; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 1; 2-2015; 121-128
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12460
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/rda.12460
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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