Immobilization of environmental bacteria using microfluidic devices and industrial byproducts

Autores
Velásquez Yánez, Paola Mishell; Lobo, Constanza Belén; Layedra, Gina; Perez, Maximiliano Sebastian; Lerner, Betiana; Juárez Tomás, María Silvina
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Environmental bacteria play a crucial role in regulating ecosystem services and can be utilized in biotechnological products with innovative applications across various fields. Our studies focus on the immobilization of environmental bacteria for use in contaminant removal processes. Lab-on-a-chip technology applied to bacterial immobilization is an emerging area, offering the advantages of system miniaturization and low-cost analysis. The aim of this work was to evaluate the immobilization, through biofilm formation, of bacteria isolated from petroleum-contaminated environments using microfluidic devices (chips) as a support and a low-cost culture medium formulated with industrial byproducts. The planktonic growth of Pseudomonas sp. P26 (P26) and Rhodococcus sp. F27 (F27), bacteria selected for their ability to remove petroleum aromatic compounds, was assessed both in LB broth (a standard culture medium) and in the alternative medium based on corn steep water and crude glycerol. Planktonic growth was assessed by enumerating colony-forming units (CFU) per millilitre. In order to immobilize bacteria, polydimethylsiloxane (PDMS) chips with a glass base and an internal chamber volume of 300 µL were fabricated. The biofilm formation of P26 and F27 in the chips (at 30°C, for 72 hours with continuous flow) was evaluated in the low-cost culture medium. Biofilm formation was analyzed using electron microscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique to determine cellular metabolic activity. The growth of planktonic cultures and bacterial immobilization in the chips were successful for both P26 and F27, with P26 forming significantly more abundant biofilms than F27. Both strains developed complex, structured biofilms with intercellular connections and a moderate amount of extracellular material (in P26). These promising results in biomass immobilization on chips, using regional industrial byproducts, pave the way for the design of bioproducts with potential for environmental contaminant removal.
Fil: Velásquez Yánez, Paola Mishell. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Layedra, Gina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Fil: Perez, Maximiliano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería; Argentina
Fil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Universidad Nacional de Córdoba
Materia
BACTERIAL BIOFILM
METABOLIC ACTIVITY
MICROCHIPS
CORN STEEP LIQUOR
CRUDE GLYCEROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276705
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276705
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of environmental bacteria using microfluidic devices and industrial byproductsVelásquez Yánez, Paola MishellLobo, Constanza BelénLayedra, GinaPerez, Maximiliano SebastianLerner, BetianaJuárez Tomás, María SilvinaBACTERIAL BIOFILMMETABOLIC ACTIVITYMICROCHIPSCORN STEEP LIQUORCRUDE GLYCEROLhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Environmental bacteria play a crucial role in regulating ecosystem services and can be utilized in biotechnological products with innovative applications across various fields. Our studies focus on the immobilization of environmental bacteria for use in contaminant removal processes. Lab-on-a-chip technology applied to bacterial immobilization is an emerging area, offering the advantages of system miniaturization and low-cost analysis. The aim of this work was to evaluate the immobilization, through biofilm formation, of bacteria isolated from petroleum-contaminated environments using microfluidic devices (chips) as a support and a low-cost culture medium formulated with industrial byproducts. The planktonic growth of Pseudomonas sp. P26 (P26) and Rhodococcus sp. F27 (F27), bacteria selected for their ability to remove petroleum aromatic compounds, was assessed both in LB broth (a standard culture medium) and in the alternative medium based on corn steep water and crude glycerol. Planktonic growth was assessed by enumerating colony-forming units (CFU) per millilitre. In order to immobilize bacteria, polydimethylsiloxane (PDMS) chips with a glass base and an internal chamber volume of 300 µL were fabricated. The biofilm formation of P26 and F27 in the chips (at 30°C, for 72 hours with continuous flow) was evaluated in the low-cost culture medium. Biofilm formation was analyzed using electron microscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique to determine cellular metabolic activity. The growth of planktonic cultures and bacterial immobilization in the chips were successful for both P26 and F27, with P26 forming significantly more abundant biofilms than F27. Both strains developed complex, structured biofilms with intercellular connections and a moderate amount of extracellular material (in P26). These promising results in biomass immobilization on chips, using regional industrial byproducts, pave the way for the design of bioproducts with potential for environmental contaminant removal.Fil: Velásquez Yánez, Paola Mishell. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Layedra, Gina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; ArgentinaFil: Perez, Maximiliano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería; ArgentinaFil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaLX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchCórdobaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularUniversidad Nacional de CórdobaSociedad Argentina de Investigaciones en Bioquímica y Biología Molecular2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276705Immobilization of environmental bacteria using microfluidic devices and industrial byproducts; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 135-136CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:17:11Zoai:ri.conicet.gov.ar:11336/276705instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:17:12.184CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
title Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
spellingShingle Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
Velásquez Yánez, Paola Mishell
BACTERIAL BIOFILM
METABOLIC ACTIVITY
MICROCHIPS
CORN STEEP LIQUOR
CRUDE GLYCEROL
title_short Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
title_full Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
title_fullStr Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
title_full_unstemmed Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
title_sort Immobilization of environmental bacteria using microfluidic devices and industrial byproducts
dc.creator.none.fl_str_mv Velásquez Yánez, Paola Mishell
Lobo, Constanza Belén
Layedra, Gina
Perez, Maximiliano Sebastian
Lerner, Betiana
Juárez Tomás, María Silvina
author Velásquez Yánez, Paola Mishell
author_facet Velásquez Yánez, Paola Mishell
Lobo, Constanza Belén
Layedra, Gina
Perez, Maximiliano Sebastian
Lerner, Betiana
Juárez Tomás, María Silvina
author_role author
author2 Lobo, Constanza Belén
Layedra, Gina
Perez, Maximiliano Sebastian
Lerner, Betiana
Juárez Tomás, María Silvina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIAL BIOFILM
METABOLIC ACTIVITY
MICROCHIPS
CORN STEEP LIQUOR
CRUDE GLYCEROL
topic BACTERIAL BIOFILM
METABOLIC ACTIVITY
MICROCHIPS
CORN STEEP LIQUOR
CRUDE GLYCEROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Environmental bacteria play a crucial role in regulating ecosystem services and can be utilized in biotechnological products with innovative applications across various fields. Our studies focus on the immobilization of environmental bacteria for use in contaminant removal processes. Lab-on-a-chip technology applied to bacterial immobilization is an emerging area, offering the advantages of system miniaturization and low-cost analysis. The aim of this work was to evaluate the immobilization, through biofilm formation, of bacteria isolated from petroleum-contaminated environments using microfluidic devices (chips) as a support and a low-cost culture medium formulated with industrial byproducts. The planktonic growth of Pseudomonas sp. P26 (P26) and Rhodococcus sp. F27 (F27), bacteria selected for their ability to remove petroleum aromatic compounds, was assessed both in LB broth (a standard culture medium) and in the alternative medium based on corn steep water and crude glycerol. Planktonic growth was assessed by enumerating colony-forming units (CFU) per millilitre. In order to immobilize bacteria, polydimethylsiloxane (PDMS) chips with a glass base and an internal chamber volume of 300 µL were fabricated. The biofilm formation of P26 and F27 in the chips (at 30°C, for 72 hours with continuous flow) was evaluated in the low-cost culture medium. Biofilm formation was analyzed using electron microscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique to determine cellular metabolic activity. The growth of planktonic cultures and bacterial immobilization in the chips were successful for both P26 and F27, with P26 forming significantly more abundant biofilms than F27. Both strains developed complex, structured biofilms with intercellular connections and a moderate amount of extracellular material (in P26). These promising results in biomass immobilization on chips, using regional industrial byproducts, pave the way for the design of bioproducts with potential for environmental contaminant removal.
Fil: Velásquez Yánez, Paola Mishell. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Layedra, Gina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Fil: Perez, Maximiliano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería; Argentina
Fil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Universidad Nacional de Córdoba
description Environmental bacteria play a crucial role in regulating ecosystem services and can be utilized in biotechnological products with innovative applications across various fields. Our studies focus on the immobilization of environmental bacteria for use in contaminant removal processes. Lab-on-a-chip technology applied to bacterial immobilization is an emerging area, offering the advantages of system miniaturization and low-cost analysis. The aim of this work was to evaluate the immobilization, through biofilm formation, of bacteria isolated from petroleum-contaminated environments using microfluidic devices (chips) as a support and a low-cost culture medium formulated with industrial byproducts. The planktonic growth of Pseudomonas sp. P26 (P26) and Rhodococcus sp. F27 (F27), bacteria selected for their ability to remove petroleum aromatic compounds, was assessed both in LB broth (a standard culture medium) and in the alternative medium based on corn steep water and crude glycerol. Planktonic growth was assessed by enumerating colony-forming units (CFU) per millilitre. In order to immobilize bacteria, polydimethylsiloxane (PDMS) chips with a glass base and an internal chamber volume of 300 µL were fabricated. The biofilm formation of P26 and F27 in the chips (at 30°C, for 72 hours with continuous flow) was evaluated in the low-cost culture medium. Biofilm formation was analyzed using electron microscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique to determine cellular metabolic activity. The growth of planktonic cultures and bacterial immobilization in the chips were successful for both P26 and F27, with P26 forming significantly more abundant biofilms than F27. Both strains developed complex, structured biofilms with intercellular connections and a moderate amount of extracellular material (in P26). These promising results in biomass immobilization on chips, using regional industrial byproducts, pave the way for the design of bioproducts with potential for environmental contaminant removal.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276705
Immobilization of environmental bacteria using microfluidic devices and industrial byproducts; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 135-136
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276705
identifier_str_mv Immobilization of environmental bacteria using microfluidic devices and industrial byproducts; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 135-136
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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