Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors

Autores
Diaz, Sebastian Andres; Gillanders, Florencia; Jares, Elizabeth Andrea; Jovin, Thomas M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optical resolution dictated by diffraction. Here we have devised a family of photoswitchable quantum dots (psQDs) in which the semiconductor core functions as a fluorescence donor in Förster resonance energy transfer (FRET), and multiple photochromic diheteroarylethene groups function as acceptors upon activation by ultraviolet light. The QDs were coated with a polymer bearing photochromic groups attached via linkers of different length. Despite the resulting nominal differences in donor-acceptor separation and anticipated FRET efficiencies, the maximum quenching of all psQD preparations was 38±2%. This result was attributable to the large ultraviolet absorption cross-section of the QDs, leading to preferential cycloreversion of photochromic groups situated closer to the nanoparticle surface and/or with a more favourable orientation.
Fil: Diaz, Sebastian Andres. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Gillanders, Florencia. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Jares, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Jovin, Thomas M.. Max Planck Institute for Biophysical Chemistry; Alemania
Materia
Physical Sciences
Nanotechnology
Optical Physics
Physical Chemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/39941
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network_name_str CONICET Digital (CONICET)
spelling Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptorsDiaz, Sebastian AndresGillanders, FlorenciaJares, Elizabeth AndreaJovin, Thomas M.Physical SciencesNanotechnologyOptical PhysicsPhysical Chemistryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optical resolution dictated by diffraction. Here we have devised a family of photoswitchable quantum dots (psQDs) in which the semiconductor core functions as a fluorescence donor in Förster resonance energy transfer (FRET), and multiple photochromic diheteroarylethene groups function as acceptors upon activation by ultraviolet light. The QDs were coated with a polymer bearing photochromic groups attached via linkers of different length. Despite the resulting nominal differences in donor-acceptor separation and anticipated FRET efficiencies, the maximum quenching of all psQD preparations was 38±2%. This result was attributable to the large ultraviolet absorption cross-section of the QDs, leading to preferential cycloreversion of photochromic groups situated closer to the nanoparticle surface and/or with a more favourable orientation.Fil: Diaz, Sebastian Andres. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Gillanders, Florencia. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Jares, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Jovin, Thomas M.. Max Planck Institute for Biophysical Chemistry; AlemaniaNature Publishing Group2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39941Diaz, Sebastian Andres; Gillanders, Florencia; Jares, Elizabeth Andrea; Jovin, Thomas M.; Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors; Nature Publishing Group; Nature Communications; 6; 1-2015; 1-112041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms7036info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms7036info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:35Zoai:ri.conicet.gov.ar:11336/39941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:35.653CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
title Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
spellingShingle Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
Diaz, Sebastian Andres
Physical Sciences
Nanotechnology
Optical Physics
Physical Chemistry
title_short Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
title_full Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
title_fullStr Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
title_full_unstemmed Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
title_sort Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
dc.creator.none.fl_str_mv Diaz, Sebastian Andres
Gillanders, Florencia
Jares, Elizabeth Andrea
Jovin, Thomas M.
author Diaz, Sebastian Andres
author_facet Diaz, Sebastian Andres
Gillanders, Florencia
Jares, Elizabeth Andrea
Jovin, Thomas M.
author_role author
author2 Gillanders, Florencia
Jares, Elizabeth Andrea
Jovin, Thomas M.
author2_role author
author
author
dc.subject.none.fl_str_mv Physical Sciences
Nanotechnology
Optical Physics
Physical Chemistry
topic Physical Sciences
Nanotechnology
Optical Physics
Physical Chemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optical resolution dictated by diffraction. Here we have devised a family of photoswitchable quantum dots (psQDs) in which the semiconductor core functions as a fluorescence donor in Förster resonance energy transfer (FRET), and multiple photochromic diheteroarylethene groups function as acceptors upon activation by ultraviolet light. The QDs were coated with a polymer bearing photochromic groups attached via linkers of different length. Despite the resulting nominal differences in donor-acceptor separation and anticipated FRET efficiencies, the maximum quenching of all psQD preparations was 38±2%. This result was attributable to the large ultraviolet absorption cross-section of the QDs, leading to preferential cycloreversion of photochromic groups situated closer to the nanoparticle surface and/or with a more favourable orientation.
Fil: Diaz, Sebastian Andres. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Gillanders, Florencia. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Jares, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Jovin, Thomas M.. Max Planck Institute for Biophysical Chemistry; Alemania
description Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optical resolution dictated by diffraction. Here we have devised a family of photoswitchable quantum dots (psQDs) in which the semiconductor core functions as a fluorescence donor in Förster resonance energy transfer (FRET), and multiple photochromic diheteroarylethene groups function as acceptors upon activation by ultraviolet light. The QDs were coated with a polymer bearing photochromic groups attached via linkers of different length. Despite the resulting nominal differences in donor-acceptor separation and anticipated FRET efficiencies, the maximum quenching of all psQD preparations was 38±2%. This result was attributable to the large ultraviolet absorption cross-section of the QDs, leading to preferential cycloreversion of photochromic groups situated closer to the nanoparticle surface and/or with a more favourable orientation.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39941
Diaz, Sebastian Andres; Gillanders, Florencia; Jares, Elizabeth Andrea; Jovin, Thomas M.; Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors; Nature Publishing Group; Nature Communications; 6; 1-2015; 1-11
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39941
identifier_str_mv Diaz, Sebastian Andres; Gillanders, Florencia; Jares, Elizabeth Andrea; Jovin, Thomas M.; Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors; Nature Publishing Group; Nature Communications; 6; 1-2015; 1-11
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms7036
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms7036
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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