Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum

Autores
Montalvillo, Enrique; Bernardo, David; Martínez Abad, Beatriz; Allegretti, Yessica Lorena; Fernández Salazar, Luis; Calvo, Carmen; Chirdo, Fernando Gabriel; Garrote, José A.; Arranz, Eduardo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Celiac Disease (CD) is an interferon (IFN)γ-mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. Vα24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients (uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls) and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were recruited. Messenger RNA (mRNA) expression of Vα24-Jα18 (invariant TCRα chain of human iNKT cells), IFNγ and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had higher Vα24-Jα18 mRNA levels than non-CD controls (I and C-controls). The expression of Vα24-Jα18 correlated with Marsh score for the severity of mucosal lesion and also with increased mRNA IFNγ levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but increased expression of Vα24-Jα18, which revealed a CD-like molecular profile. Increased numbers of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment of uCD and GFD-CD patients and correlated with Vα24-Jα18 mRNA expression. In conclusion, we have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased mRNA expression of Vα24-Jα18 together with a decreased expression of FoxP3, may represent a pro-inflammatory signature of the CD duodenum.
Fil: Montalvillo, Enrique. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Bernardo, David. Imperial College London; Reino Unido. Hospital Universitario de La Princesa ; Países Bajos
Fil: Martínez Abad, Beatriz. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España. Instituto de Investigación Sanitaria Princesa; España
Fil: Allegretti, Yessica Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Fernández Salazar, Luis. Hospital Clínico Universitario; España
Fil: Calvo, Carmen. Hospital Clínico Universitario; España
Fil: Chirdo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Garrote, José A.. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España. Hospital Universitario Rio Hortega; España
Fil: Arranz, Eduardo. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España
Materia
CELIAC DISEASE
CELIAC DISEASE-LIKE MOLECULAR PROFILE
IFNΓ
INKT
INTRAEPITHELIAL LYMPHOCYTES
VΑ24-JΑ18
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54435
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54435
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenumMontalvillo, EnriqueBernardo, DavidMartínez Abad, BeatrizAllegretti, Yessica LorenaFernández Salazar, LuisCalvo, CarmenChirdo, Fernando GabrielGarrote, José A.Arranz, EduardoCELIAC DISEASECELIAC DISEASE-LIKE MOLECULAR PROFILEIFNΓINKTINTRAEPITHELIAL LYMPHOCYTESVΑ24-JΑ18https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Celiac Disease (CD) is an interferon (IFN)γ-mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. Vα24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients (uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls) and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were recruited. Messenger RNA (mRNA) expression of Vα24-Jα18 (invariant TCRα chain of human iNKT cells), IFNγ and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had higher Vα24-Jα18 mRNA levels than non-CD controls (I and C-controls). The expression of Vα24-Jα18 correlated with Marsh score for the severity of mucosal lesion and also with increased mRNA IFNγ levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but increased expression of Vα24-Jα18, which revealed a CD-like molecular profile. Increased numbers of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment of uCD and GFD-CD patients and correlated with Vα24-Jα18 mRNA expression. In conclusion, we have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased mRNA expression of Vα24-Jα18 together with a decreased expression of FoxP3, may represent a pro-inflammatory signature of the CD duodenum.Fil: Montalvillo, Enrique. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Bernardo, David. Imperial College London; Reino Unido. Hospital Universitario de La Princesa ; Países BajosFil: Martínez Abad, Beatriz. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España. Instituto de Investigación Sanitaria Princesa; EspañaFil: Allegretti, Yessica Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; ArgentinaFil: Fernández Salazar, Luis. Hospital Clínico Universitario; EspañaFil: Calvo, Carmen. Hospital Clínico Universitario; EspañaFil: Chirdo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; ArgentinaFil: Garrote, José A.. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España. Hospital Universitario Rio Hortega; EspañaFil: Arranz, Eduardo. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; EspañaMDPI AG2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54435Montalvillo, Enrique; Bernardo, David; Martínez Abad, Beatriz; Allegretti, Yessica Lorena; Fernández Salazar, Luis; et al.; Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum; MDPI AG; Nutrients; 7; 11; 10-2015; 8960-89762072-6643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/nu7115444info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6643/7/11/5444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:53Zoai:ri.conicet.gov.ar:11336/54435instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:53.738CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
spellingShingle Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
Montalvillo, Enrique
CELIAC DISEASE
CELIAC DISEASE-LIKE MOLECULAR PROFILE
IFNΓ
INKT
INTRAEPITHELIAL LYMPHOCYTES
VΑ24-JΑ18
title_short Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_full Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_fullStr Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_full_unstemmed Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_sort Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
dc.creator.none.fl_str_mv Montalvillo, Enrique
Bernardo, David
Martínez Abad, Beatriz
Allegretti, Yessica Lorena
Fernández Salazar, Luis
Calvo, Carmen
Chirdo, Fernando Gabriel
Garrote, José A.
Arranz, Eduardo
author Montalvillo, Enrique
author_facet Montalvillo, Enrique
Bernardo, David
Martínez Abad, Beatriz
Allegretti, Yessica Lorena
Fernández Salazar, Luis
Calvo, Carmen
Chirdo, Fernando Gabriel
Garrote, José A.
Arranz, Eduardo
author_role author
author2 Bernardo, David
Martínez Abad, Beatriz
Allegretti, Yessica Lorena
Fernández Salazar, Luis
Calvo, Carmen
Chirdo, Fernando Gabriel
Garrote, José A.
Arranz, Eduardo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELIAC DISEASE
CELIAC DISEASE-LIKE MOLECULAR PROFILE
IFNΓ
INKT
INTRAEPITHELIAL LYMPHOCYTES
VΑ24-JΑ18
topic CELIAC DISEASE
CELIAC DISEASE-LIKE MOLECULAR PROFILE
IFNΓ
INKT
INTRAEPITHELIAL LYMPHOCYTES
VΑ24-JΑ18
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Celiac Disease (CD) is an interferon (IFN)γ-mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. Vα24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients (uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls) and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were recruited. Messenger RNA (mRNA) expression of Vα24-Jα18 (invariant TCRα chain of human iNKT cells), IFNγ and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had higher Vα24-Jα18 mRNA levels than non-CD controls (I and C-controls). The expression of Vα24-Jα18 correlated with Marsh score for the severity of mucosal lesion and also with increased mRNA IFNγ levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but increased expression of Vα24-Jα18, which revealed a CD-like molecular profile. Increased numbers of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment of uCD and GFD-CD patients and correlated with Vα24-Jα18 mRNA expression. In conclusion, we have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased mRNA expression of Vα24-Jα18 together with a decreased expression of FoxP3, may represent a pro-inflammatory signature of the CD duodenum.
Fil: Montalvillo, Enrique. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Bernardo, David. Imperial College London; Reino Unido. Hospital Universitario de La Princesa ; Países Bajos
Fil: Martínez Abad, Beatriz. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España. Instituto de Investigación Sanitaria Princesa; España
Fil: Allegretti, Yessica Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Fernández Salazar, Luis. Hospital Clínico Universitario; España
Fil: Calvo, Carmen. Hospital Clínico Universitario; España
Fil: Chirdo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Garrote, José A.. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España. Hospital Universitario Rio Hortega; España
Fil: Arranz, Eduardo. Universidad de Valladolid; España. Consejo Superior de Investigaciones Científicas; España
description Celiac Disease (CD) is an interferon (IFN)γ-mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. Vα24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients (uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls) and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were recruited. Messenger RNA (mRNA) expression of Vα24-Jα18 (invariant TCRα chain of human iNKT cells), IFNγ and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had higher Vα24-Jα18 mRNA levels than non-CD controls (I and C-controls). The expression of Vα24-Jα18 correlated with Marsh score for the severity of mucosal lesion and also with increased mRNA IFNγ levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but increased expression of Vα24-Jα18, which revealed a CD-like molecular profile. Increased numbers of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment of uCD and GFD-CD patients and correlated with Vα24-Jα18 mRNA expression. In conclusion, we have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased mRNA expression of Vα24-Jα18 together with a decreased expression of FoxP3, may represent a pro-inflammatory signature of the CD duodenum.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54435
Montalvillo, Enrique; Bernardo, David; Martínez Abad, Beatriz; Allegretti, Yessica Lorena; Fernández Salazar, Luis; et al.; Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum; MDPI AG; Nutrients; 7; 11; 10-2015; 8960-8976
2072-6643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54435
identifier_str_mv Montalvillo, Enrique; Bernardo, David; Martínez Abad, Beatriz; Allegretti, Yessica Lorena; Fernández Salazar, Luis; et al.; Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum; MDPI AG; Nutrients; 7; 11; 10-2015; 8960-8976
2072-6643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/nu7115444
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6643/7/11/5444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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