Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)

Autores
Attademo, Andres Maximiliano; Peltzer, Paola; Lajmanovich, Rafael Carlos; Basso, Agustin; Junges, Celina Maria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We determined basal levels of cholinesterase (ChE) and carboxylesterase (CbEs; two substrates: α- naphthyl acetate and 4-nitrophenylvalerate) in different tissues of tadpoles and adults of the frog Pseudis paradoxa and evaluated their use as complementary biomarkers of anti-cholinesterase pesticide exposure. ChE and CbEs sensitivity to malaoxon was also evaluated. Adults and tadpoles were collected with sweep net from temporary ponds located in natural riparian forests along the Paraná River (Garay Department, Santa Fe province, Argentina). We found significant differences in B-esterase activities between adults and tadpoles and among different tissues. The in vitro inhibition tests indicated that ChE is more sensitive to inhibition than CbEs. Our results suggest that basal ChE and CbE (α-NA and 4- NPV) activities in different tissues of adult and tadpoles P. paradoxa would be suitable biomarkers of pesticide exposure, and this amphibian species could be used as sentinel in field monitoring.
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Fil: Basso, Agustin. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Materia
CHOLINESTERASE
CARBOXYLESTERASE
MALAOXON
PSEUDIS PARADOXA
BIOMARKER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92775
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)Attademo, Andres MaximilianoPeltzer, PaolaLajmanovich, Rafael CarlosBasso, AgustinJunges, Celina MariaCHOLINESTERASECARBOXYLESTERASEMALAOXONPSEUDIS PARADOXABIOMARKERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We determined basal levels of cholinesterase (ChE) and carboxylesterase (CbEs; two substrates: α- naphthyl acetate and 4-nitrophenylvalerate) in different tissues of tadpoles and adults of the frog Pseudis paradoxa and evaluated their use as complementary biomarkers of anti-cholinesterase pesticide exposure. ChE and CbEs sensitivity to malaoxon was also evaluated. Adults and tadpoles were collected with sweep net from temporary ponds located in natural riparian forests along the Paraná River (Garay Department, Santa Fe province, Argentina). We found significant differences in B-esterase activities between adults and tadpoles and among different tissues. The in vitro inhibition tests indicated that ChE is more sensitive to inhibition than CbEs. Our results suggest that basal ChE and CbE (α-NA and 4- NPV) activities in different tissues of adult and tadpoles P. paradoxa would be suitable biomarkers of pesticide exposure, and this amphibian species could be used as sentinel in field monitoring.Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Basso, Agustin. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaSpringer2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92775Attademo, Andres Maximiliano; Peltzer, Paola; Lajmanovich, Rafael Carlos; Basso, Agustin; Junges, Celina Maria; Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae); Springer; Water, Air and Soil Pollution; 225; 3; 2-2014; 1903-19121567-7230CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11270-014-1903-3info:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-014-1903-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:09Zoai:ri.conicet.gov.ar:11336/92775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:09.718CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
title Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
spellingShingle Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
Attademo, Andres Maximiliano
CHOLINESTERASE
CARBOXYLESTERASE
MALAOXON
PSEUDIS PARADOXA
BIOMARKER
title_short Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
title_full Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
title_fullStr Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
title_full_unstemmed Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
title_sort Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
dc.creator.none.fl_str_mv Attademo, Andres Maximiliano
Peltzer, Paola
Lajmanovich, Rafael Carlos
Basso, Agustin
Junges, Celina Maria
author Attademo, Andres Maximiliano
author_facet Attademo, Andres Maximiliano
Peltzer, Paola
Lajmanovich, Rafael Carlos
Basso, Agustin
Junges, Celina Maria
author_role author
author2 Peltzer, Paola
Lajmanovich, Rafael Carlos
Basso, Agustin
Junges, Celina Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHOLINESTERASE
CARBOXYLESTERASE
MALAOXON
PSEUDIS PARADOXA
BIOMARKER
topic CHOLINESTERASE
CARBOXYLESTERASE
MALAOXON
PSEUDIS PARADOXA
BIOMARKER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We determined basal levels of cholinesterase (ChE) and carboxylesterase (CbEs; two substrates: α- naphthyl acetate and 4-nitrophenylvalerate) in different tissues of tadpoles and adults of the frog Pseudis paradoxa and evaluated their use as complementary biomarkers of anti-cholinesterase pesticide exposure. ChE and CbEs sensitivity to malaoxon was also evaluated. Adults and tadpoles were collected with sweep net from temporary ponds located in natural riparian forests along the Paraná River (Garay Department, Santa Fe province, Argentina). We found significant differences in B-esterase activities between adults and tadpoles and among different tissues. The in vitro inhibition tests indicated that ChE is more sensitive to inhibition than CbEs. Our results suggest that basal ChE and CbE (α-NA and 4- NPV) activities in different tissues of adult and tadpoles P. paradoxa would be suitable biomarkers of pesticide exposure, and this amphibian species could be used as sentinel in field monitoring.
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
Fil: Basso, Agustin. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina
description We determined basal levels of cholinesterase (ChE) and carboxylesterase (CbEs; two substrates: α- naphthyl acetate and 4-nitrophenylvalerate) in different tissues of tadpoles and adults of the frog Pseudis paradoxa and evaluated their use as complementary biomarkers of anti-cholinesterase pesticide exposure. ChE and CbEs sensitivity to malaoxon was also evaluated. Adults and tadpoles were collected with sweep net from temporary ponds located in natural riparian forests along the Paraná River (Garay Department, Santa Fe province, Argentina). We found significant differences in B-esterase activities between adults and tadpoles and among different tissues. The in vitro inhibition tests indicated that ChE is more sensitive to inhibition than CbEs. Our results suggest that basal ChE and CbE (α-NA and 4- NPV) activities in different tissues of adult and tadpoles P. paradoxa would be suitable biomarkers of pesticide exposure, and this amphibian species could be used as sentinel in field monitoring.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92775
Attademo, Andres Maximiliano; Peltzer, Paola; Lajmanovich, Rafael Carlos; Basso, Agustin; Junges, Celina Maria; Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae); Springer; Water, Air and Soil Pollution; 225; 3; 2-2014; 1903-1912
1567-7230
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92775
identifier_str_mv Attademo, Andres Maximiliano; Peltzer, Paola; Lajmanovich, Rafael Carlos; Basso, Agustin; Junges, Celina Maria; Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae); Springer; Water, Air and Soil Pollution; 225; 3; 2-2014; 1903-1912
1567-7230
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11270-014-1903-3
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-014-1903-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269139457015808
score 13.13397

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