Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction

Autores
Montiel Jarillo, Gabriela; Morales Urrea, Diego Alberto; Contreras, Edgardo Martin; López Córdoba, Alex Fernando; Gómez Pachón, Edwin Yesid; Carrera, Julián; Suárez Ojeda, María Eugenia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by 1H and 13C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB.
Fil: Montiel Jarillo, Gabriela. Universitat Autònoma de Barcelona; España
Fil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: López Córdoba, Alex Fernando. Universidad Pedagógica y Tecnológica de Colombia; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gómez Pachón, Edwin Yesid. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Carrera, Julián. Universitat Autònoma de Barcelona; España
Fil: Suárez Ojeda, María Eugenia. Universitat Autònoma de Barcelona; España
Materia
CHLOROFORM
DIMETHYL CARBONATE
EXTRACTION
MIXED MICROBIAL CULTURES
POLYHYDROXYALKANOATES
SODIUM HYPOCHLORITE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212006
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212006
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent ExtractionMontiel Jarillo, GabrielaMorales Urrea, Diego AlbertoContreras, Edgardo MartinLópez Córdoba, Alex FernandoGómez Pachón, Edwin YesidCarrera, JuliánSuárez Ojeda, María EugeniaCHLOROFORMDIMETHYL CARBONATEEXTRACTIONMIXED MICROBIAL CULTURESPOLYHYDROXYALKANOATESSODIUM HYPOCHLORITEhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by 1H and 13C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB.Fil: Montiel Jarillo, Gabriela. Universitat Autònoma de Barcelona; EspañaFil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: López Córdoba, Alex Fernando. Universidad Pedagógica y Tecnológica de Colombia; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gómez Pachón, Edwin Yesid. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Carrera, Julián. Universitat Autònoma de Barcelona; EspañaFil: Suárez Ojeda, María Eugenia. Universitat Autònoma de Barcelona; EspañaMultidisciplinary Digital Publishing Institute2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212006Montiel Jarillo, Gabriela; Morales Urrea, Diego Alberto; Contreras, Edgardo Martin; López Córdoba, Alex Fernando; Gómez Pachón, Edwin Yesid; et al.; Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction; Multidisciplinary Digital Publishing Institute; Polymers; 14; 19; 9-2022; 1-172073-4360CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/14/19/3938info:eu-repo/semantics/altIdentifier/doi/10.3390/polym14193938info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:04:00Zoai:ri.conicet.gov.ar:11336/212006instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:04:01.075CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
title Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
spellingShingle Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
Montiel Jarillo, Gabriela
CHLOROFORM
DIMETHYL CARBONATE
EXTRACTION
MIXED MICROBIAL CULTURES
POLYHYDROXYALKANOATES
SODIUM HYPOCHLORITE
title_short Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
title_full Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
title_fullStr Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
title_full_unstemmed Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
title_sort Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
dc.creator.none.fl_str_mv Montiel Jarillo, Gabriela
Morales Urrea, Diego Alberto
Contreras, Edgardo Martin
López Córdoba, Alex Fernando
Gómez Pachón, Edwin Yesid
Carrera, Julián
Suárez Ojeda, María Eugenia
author Montiel Jarillo, Gabriela
author_facet Montiel Jarillo, Gabriela
Morales Urrea, Diego Alberto
Contreras, Edgardo Martin
López Córdoba, Alex Fernando
Gómez Pachón, Edwin Yesid
Carrera, Julián
Suárez Ojeda, María Eugenia
author_role author
author2 Morales Urrea, Diego Alberto
Contreras, Edgardo Martin
López Córdoba, Alex Fernando
Gómez Pachón, Edwin Yesid
Carrera, Julián
Suárez Ojeda, María Eugenia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHLOROFORM
DIMETHYL CARBONATE
EXTRACTION
MIXED MICROBIAL CULTURES
POLYHYDROXYALKANOATES
SODIUM HYPOCHLORITE
topic CHLOROFORM
DIMETHYL CARBONATE
EXTRACTION
MIXED MICROBIAL CULTURES
POLYHYDROXYALKANOATES
SODIUM HYPOCHLORITE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by 1H and 13C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB.
Fil: Montiel Jarillo, Gabriela. Universitat Autònoma de Barcelona; España
Fil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: López Córdoba, Alex Fernando. Universidad Pedagógica y Tecnológica de Colombia; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gómez Pachón, Edwin Yesid. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Carrera, Julián. Universitat Autònoma de Barcelona; España
Fil: Suárez Ojeda, María Eugenia. Universitat Autònoma de Barcelona; España
description The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by 1H and 13C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212006
Montiel Jarillo, Gabriela; Morales Urrea, Diego Alberto; Contreras, Edgardo Martin; López Córdoba, Alex Fernando; Gómez Pachón, Edwin Yesid; et al.; Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction; Multidisciplinary Digital Publishing Institute; Polymers; 14; 19; 9-2022; 1-17
2073-4360
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212006
identifier_str_mv Montiel Jarillo, Gabriela; Morales Urrea, Diego Alberto; Contreras, Edgardo Martin; López Córdoba, Alex Fernando; Gómez Pachón, Edwin Yesid; et al.; Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction; Multidisciplinary Digital Publishing Institute; Polymers; 14; 19; 9-2022; 1-17
2073-4360
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/14/19/3938
info:eu-repo/semantics/altIdentifier/doi/10.3390/polym14193938
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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