First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region

Autores
Lizasoain, Andrés; Mir, Daiana; Salvo, Matías; Bortagaray, Viviana; Masachessi, Gisela; Farías, Adrián Alejandro; Rodríguez Osorio, Nélida; Nates, Silvia Viviana; Victoria, Matías; Colina, Rodney
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human enteroviruses (EVs) comprise more than 100 types of coxsackievirus, echovirus, poliovirus and numbered enteroviruses, which are mainly transmitted by the faecal-oral route leading to diverse diseases such as aseptic meningitis, encephalitis, and acute flaccid paralysis, among others. Since enteroviruses are excreted in faeces, wastewater-based epidemiology approaches are useful to describe EV diversity in a community. In Uruguay, knowledge about enteroviruses is extremely limited. This study assessed the diversity of enteroviruses through Illumina next-generation sequencing of VP1-amplicons obtained by RT-PCR directly applied to viral concentrates of 84 wastewater samples collected in Uruguay during 2011–2012 and 2017–2018. Fifty out of the 84 samples were positive for enteroviruses. There were detected 27 different types belonging to Enterovirus A species (CVA2-A6, A10, A16, EV-A71, A90), Enterovirus B species (CVA9, B1-B5, E1, E6, E11, E14, E21, E30) and Enterovirus C species (CVA1, A13, A19, A22, A24, EV-C99). Enterovirus A71 (EV-A71) and echovirus 30 (E30) strains were studied more in depth through phylogenetic analysis, together with some strains previously detected by us in Argentina. Results unveiled that EV-A71 sub-genogroup C2 circulates in both countries at least since 2011–2012, and that the C1-like emerging variant recently entered in Argentina. We also confirmed the circulation of echovirus 30 genotypes E and F in Argentina, and reported the detection of genotype E in Uruguay. To the best of our knowledge this is the first report of the EV-A71 C1-like emerging variant in South-America, and the first report of EV-A71 and E30 in Uruguay.
Fil: Lizasoain, Andrés. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Mir, Daiana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Salvo, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Bortagaray, Viviana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Rodríguez Osorio, Nélida. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Victoria, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Colina, Rodney. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Materia
ENTEROVIRUS A71
ECHOVIRUS 30
LLUMINA NEXT-GENERATION SEQUENCING
WASTEWATER SAMPLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158717
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American regionLizasoain, AndrésMir, DaianaSalvo, MatíasBortagaray, VivianaMasachessi, GiselaFarías, Adrián AlejandroRodríguez Osorio, NélidaNates, Silvia VivianaVictoria, MatíasColina, RodneyENTEROVIRUS A71ECHOVIRUS 30LLUMINA NEXT-GENERATION SEQUENCINGWASTEWATER SAMPLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human enteroviruses (EVs) comprise more than 100 types of coxsackievirus, echovirus, poliovirus and numbered enteroviruses, which are mainly transmitted by the faecal-oral route leading to diverse diseases such as aseptic meningitis, encephalitis, and acute flaccid paralysis, among others. Since enteroviruses are excreted in faeces, wastewater-based epidemiology approaches are useful to describe EV diversity in a community. In Uruguay, knowledge about enteroviruses is extremely limited. This study assessed the diversity of enteroviruses through Illumina next-generation sequencing of VP1-amplicons obtained by RT-PCR directly applied to viral concentrates of 84 wastewater samples collected in Uruguay during 2011–2012 and 2017–2018. Fifty out of the 84 samples were positive for enteroviruses. There were detected 27 different types belonging to Enterovirus A species (CVA2-A6, A10, A16, EV-A71, A90), Enterovirus B species (CVA9, B1-B5, E1, E6, E11, E14, E21, E30) and Enterovirus C species (CVA1, A13, A19, A22, A24, EV-C99). Enterovirus A71 (EV-A71) and echovirus 30 (E30) strains were studied more in depth through phylogenetic analysis, together with some strains previously detected by us in Argentina. Results unveiled that EV-A71 sub-genogroup C2 circulates in both countries at least since 2011–2012, and that the C1-like emerging variant recently entered in Argentina. We also confirmed the circulation of echovirus 30 genotypes E and F in Argentina, and reported the detection of genotype E in Uruguay. To the best of our knowledge this is the first report of the EV-A71 C1-like emerging variant in South-America, and the first report of EV-A71 and E30 in Uruguay.Fil: Lizasoain, Andrés. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Mir, Daiana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Salvo, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Bortagaray, Viviana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Rodríguez Osorio, Nélida. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Victoria, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Colina, Rodney. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayPublic Library of Science2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158717Lizasoain, Andrés; Mir, Daiana; Salvo, Matías; Bortagaray, Viviana; Masachessi, Gisela; et al.; First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region; Public Library of Science; Plos One; 16; 8; 8-2021; 1-191932-62031932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255846info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0255846info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:00Zoai:ri.conicet.gov.ar:11336/158717instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:01.057CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
title First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
spellingShingle First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
Lizasoain, Andrés
ENTEROVIRUS A71
ECHOVIRUS 30
LLUMINA NEXT-GENERATION SEQUENCING
WASTEWATER SAMPLES
title_short First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
title_full First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
title_fullStr First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
title_full_unstemmed First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
title_sort First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region
dc.creator.none.fl_str_mv Lizasoain, Andrés
Mir, Daiana
Salvo, Matías
Bortagaray, Viviana
Masachessi, Gisela
Farías, Adrián Alejandro
Rodríguez Osorio, Nélida
Nates, Silvia Viviana
Victoria, Matías
Colina, Rodney
author Lizasoain, Andrés
author_facet Lizasoain, Andrés
Mir, Daiana
Salvo, Matías
Bortagaray, Viviana
Masachessi, Gisela
Farías, Adrián Alejandro
Rodríguez Osorio, Nélida
Nates, Silvia Viviana
Victoria, Matías
Colina, Rodney
author_role author
author2 Mir, Daiana
Salvo, Matías
Bortagaray, Viviana
Masachessi, Gisela
Farías, Adrián Alejandro
Rodríguez Osorio, Nélida
Nates, Silvia Viviana
Victoria, Matías
Colina, Rodney
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTEROVIRUS A71
ECHOVIRUS 30
LLUMINA NEXT-GENERATION SEQUENCING
WASTEWATER SAMPLES
topic ENTEROVIRUS A71
ECHOVIRUS 30
LLUMINA NEXT-GENERATION SEQUENCING
WASTEWATER SAMPLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human enteroviruses (EVs) comprise more than 100 types of coxsackievirus, echovirus, poliovirus and numbered enteroviruses, which are mainly transmitted by the faecal-oral route leading to diverse diseases such as aseptic meningitis, encephalitis, and acute flaccid paralysis, among others. Since enteroviruses are excreted in faeces, wastewater-based epidemiology approaches are useful to describe EV diversity in a community. In Uruguay, knowledge about enteroviruses is extremely limited. This study assessed the diversity of enteroviruses through Illumina next-generation sequencing of VP1-amplicons obtained by RT-PCR directly applied to viral concentrates of 84 wastewater samples collected in Uruguay during 2011–2012 and 2017–2018. Fifty out of the 84 samples were positive for enteroviruses. There were detected 27 different types belonging to Enterovirus A species (CVA2-A6, A10, A16, EV-A71, A90), Enterovirus B species (CVA9, B1-B5, E1, E6, E11, E14, E21, E30) and Enterovirus C species (CVA1, A13, A19, A22, A24, EV-C99). Enterovirus A71 (EV-A71) and echovirus 30 (E30) strains were studied more in depth through phylogenetic analysis, together with some strains previously detected by us in Argentina. Results unveiled that EV-A71 sub-genogroup C2 circulates in both countries at least since 2011–2012, and that the C1-like emerging variant recently entered in Argentina. We also confirmed the circulation of echovirus 30 genotypes E and F in Argentina, and reported the detection of genotype E in Uruguay. To the best of our knowledge this is the first report of the EV-A71 C1-like emerging variant in South-America, and the first report of EV-A71 and E30 in Uruguay.
Fil: Lizasoain, Andrés. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Mir, Daiana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Salvo, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Bortagaray, Viviana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Rodríguez Osorio, Nélida. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Victoria, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Colina, Rodney. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
description Human enteroviruses (EVs) comprise more than 100 types of coxsackievirus, echovirus, poliovirus and numbered enteroviruses, which are mainly transmitted by the faecal-oral route leading to diverse diseases such as aseptic meningitis, encephalitis, and acute flaccid paralysis, among others. Since enteroviruses are excreted in faeces, wastewater-based epidemiology approaches are useful to describe EV diversity in a community. In Uruguay, knowledge about enteroviruses is extremely limited. This study assessed the diversity of enteroviruses through Illumina next-generation sequencing of VP1-amplicons obtained by RT-PCR directly applied to viral concentrates of 84 wastewater samples collected in Uruguay during 2011–2012 and 2017–2018. Fifty out of the 84 samples were positive for enteroviruses. There were detected 27 different types belonging to Enterovirus A species (CVA2-A6, A10, A16, EV-A71, A90), Enterovirus B species (CVA9, B1-B5, E1, E6, E11, E14, E21, E30) and Enterovirus C species (CVA1, A13, A19, A22, A24, EV-C99). Enterovirus A71 (EV-A71) and echovirus 30 (E30) strains were studied more in depth through phylogenetic analysis, together with some strains previously detected by us in Argentina. Results unveiled that EV-A71 sub-genogroup C2 circulates in both countries at least since 2011–2012, and that the C1-like emerging variant recently entered in Argentina. We also confirmed the circulation of echovirus 30 genotypes E and F in Argentina, and reported the detection of genotype E in Uruguay. To the best of our knowledge this is the first report of the EV-A71 C1-like emerging variant in South-America, and the first report of EV-A71 and E30 in Uruguay.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158717
Lizasoain, Andrés; Mir, Daiana; Salvo, Matías; Bortagaray, Viviana; Masachessi, Gisela; et al.; First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region; Public Library of Science; Plos One; 16; 8; 8-2021; 1-19
1932-6203
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158717
identifier_str_mv Lizasoain, Andrés; Mir, Daiana; Salvo, Matías; Bortagaray, Viviana; Masachessi, Gisela; et al.; First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region; Public Library of Science; Plos One; 16; 8; 8-2021; 1-19
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0255846
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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