MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex

Autores
Capmany, Anahi; Yoshimura, Azumi; Kerdous, Rachid; Caorsi, Valentina; Lescure, Aurianne; Nery, Elaine Del; Coudrier, Evelyne; Goud, Bruno; Schauer, Kristine
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of MYO1C induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots. MYO1C depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of MYO1C depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.This article has an associated First Person interview with the first author of the paper.
Fil: Capmany, Anahi. Institute Curie; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Inmunologia; Argentina
Fil: Yoshimura, Azumi. Institute Curie; Francia
Fil: Kerdous, Rachid. Institute Curie; Francia
Fil: Caorsi, Valentina. Abbelight; Francia
Fil: Lescure, Aurianne. Institute Curie; Francia
Fil: Nery, Elaine Del. Institute Curie; Francia
Fil: Coudrier, Evelyne. Institute Curie; Francia
Fil: Goud, Bruno. Institute Curie; Francia
Fil: Schauer, Kristine. Institute Curie; Francia
Materia
ARP2/3
GOLGI APPARATUS
MYH9
MYO18A
MYO6
MYOSIN IIA
MYOSIN1
PLECKSTRIN HOLOLOGY DOMAIN
RAB11
RAB6
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121326
Acceder
id CONICETDig_af0165ff7b307789fc411bd1a5093a42
oai_identifier_str oai:ri.conicet.gov.ar:11336/121326
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complexCapmany, AnahiYoshimura, AzumiKerdous, RachidCaorsi, ValentinaLescure, AurianneNery, Elaine DelCoudrier, EvelyneGoud, BrunoSchauer, KristineARP2/3GOLGI APPARATUSMYH9MYO18AMYO6MYOSIN IIAMYOSIN1PLECKSTRIN HOLOLOGY DOMAINRAB11RAB6https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of MYO1C induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots. MYO1C depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of MYO1C depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.This article has an associated First Person interview with the first author of the paper.Fil: Capmany, Anahi. Institute Curie; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Inmunologia; ArgentinaFil: Yoshimura, Azumi. Institute Curie; FranciaFil: Kerdous, Rachid. Institute Curie; FranciaFil: Caorsi, Valentina. Abbelight; FranciaFil: Lescure, Aurianne. Institute Curie; FranciaFil: Nery, Elaine Del. Institute Curie; FranciaFil: Coudrier, Evelyne. Institute Curie; FranciaFil: Goud, Bruno. Institute Curie; FranciaFil: Schauer, Kristine. Institute Curie; FranciaCompany of Biologists2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121326Capmany, Anahi; Yoshimura, Azumi; Kerdous, Rachid; Caorsi, Valentina; Lescure, Aurianne; et al.; MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex; Company of Biologists; Journal of Cell Science; 132; 8; 4-2019; 1-460021-95331477-9137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://jcs.biologists.org/content/132/8/jcs225029info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.225029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:40Zoai:ri.conicet.gov.ar:11336/121326instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:41.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
title MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
spellingShingle MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
Capmany, Anahi
ARP2/3
GOLGI APPARATUS
MYH9
MYO18A
MYO6
MYOSIN IIA
MYOSIN1
PLECKSTRIN HOLOLOGY DOMAIN
RAB11
RAB6
title_short MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
title_full MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
title_fullStr MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
title_full_unstemmed MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
title_sort MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
dc.creator.none.fl_str_mv Capmany, Anahi
Yoshimura, Azumi
Kerdous, Rachid
Caorsi, Valentina
Lescure, Aurianne
Nery, Elaine Del
Coudrier, Evelyne
Goud, Bruno
Schauer, Kristine
author Capmany, Anahi
author_facet Capmany, Anahi
Yoshimura, Azumi
Kerdous, Rachid
Caorsi, Valentina
Lescure, Aurianne
Nery, Elaine Del
Coudrier, Evelyne
Goud, Bruno
Schauer, Kristine
author_role author
author2 Yoshimura, Azumi
Kerdous, Rachid
Caorsi, Valentina
Lescure, Aurianne
Nery, Elaine Del
Coudrier, Evelyne
Goud, Bruno
Schauer, Kristine
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARP2/3
GOLGI APPARATUS
MYH9
MYO18A
MYO6
MYOSIN IIA
MYOSIN1
PLECKSTRIN HOLOLOGY DOMAIN
RAB11
RAB6
topic ARP2/3
GOLGI APPARATUS
MYH9
MYO18A
MYO6
MYOSIN IIA
MYOSIN1
PLECKSTRIN HOLOLOGY DOMAIN
RAB11
RAB6
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of MYO1C induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots. MYO1C depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of MYO1C depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.This article has an associated First Person interview with the first author of the paper.
Fil: Capmany, Anahi. Institute Curie; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Inmunologia; Argentina
Fil: Yoshimura, Azumi. Institute Curie; Francia
Fil: Kerdous, Rachid. Institute Curie; Francia
Fil: Caorsi, Valentina. Abbelight; Francia
Fil: Lescure, Aurianne. Institute Curie; Francia
Fil: Nery, Elaine Del. Institute Curie; Francia
Fil: Coudrier, Evelyne. Institute Curie; Francia
Fil: Goud, Bruno. Institute Curie; Francia
Fil: Schauer, Kristine. Institute Curie; Francia
description In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of MYO1C induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots. MYO1C depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of MYO1C depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.This article has an associated First Person interview with the first author of the paper.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121326
Capmany, Anahi; Yoshimura, Azumi; Kerdous, Rachid; Caorsi, Valentina; Lescure, Aurianne; et al.; MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex; Company of Biologists; Journal of Cell Science; 132; 8; 4-2019; 1-46
0021-9533
1477-9137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121326
identifier_str_mv Capmany, Anahi; Yoshimura, Azumi; Kerdous, Rachid; Caorsi, Valentina; Lescure, Aurianne; et al.; MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex; Company of Biologists; Journal of Cell Science; 132; 8; 4-2019; 1-46
0021-9533
1477-9137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://jcs.biologists.org/content/132/8/jcs225029
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.225029
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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