Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
- Autores
- Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. University of Illinois at Urbana; Estados Unidos
Fil: Gelfand, Vladimir I.. Northwestern University; Estados Unidos
Fil: Aguirre, Ivanna Verónica. Northwestern University; Estados Unidos
Fil: Gratton, Enrico. University of Illinois at Urbana; Estados Unidos - Materia
- myosin
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/100329
Ver los metadatos del registro completo
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Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm stepsLevi, ValeriaGelfand, Vladimir I.Aguirre, Ivanna VerónicaGratton, Enricomyosinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. University of Illinois at Urbana; Estados UnidosFil: Gelfand, Vladimir I.. Northwestern University; Estados UnidosFil: Aguirre, Ivanna Verónica. Northwestern University; Estados UnidosFil: Gratton, Enrico. University of Illinois at Urbana; Estados UnidosCell Press2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100329Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico; Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps; Cell Press; Biophysical Journal; 90; 1; 12-2006; 7-90006-3495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cell.com/biophysj/fulltext/S0006-3495(06)72184-1info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367043/info:eu-repo/semantics/altIdentifier/doi/10.1529/biophysj.105.075556info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:23:42Zoai:ri.conicet.gov.ar:11336/100329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:23:42.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| title |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| spellingShingle |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps Levi, Valeria myosin |
| title_short |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| title_full |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| title_fullStr |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| title_full_unstemmed |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| title_sort |
Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps |
| dc.creator.none.fl_str_mv |
Levi, Valeria Gelfand, Vladimir I. Aguirre, Ivanna Verónica Gratton, Enrico |
| author |
Levi, Valeria |
| author_facet |
Levi, Valeria Gelfand, Vladimir I. Aguirre, Ivanna Verónica Gratton, Enrico |
| author_role |
author |
| author2 |
Gelfand, Vladimir I. Aguirre, Ivanna Verónica Gratton, Enrico |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
myosin |
| topic |
myosin |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion. Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. University of Illinois at Urbana; Estados Unidos Fil: Gelfand, Vladimir I.. Northwestern University; Estados Unidos Fil: Aguirre, Ivanna Verónica. Northwestern University; Estados Unidos Fil: Gratton, Enrico. University of Illinois at Urbana; Estados Unidos |
| description |
We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion. |
| publishDate |
2006 |
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2006-12 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://hdl.handle.net/11336/100329 Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico; Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps; Cell Press; Biophysical Journal; 90; 1; 12-2006; 7-9 0006-3495 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/100329 |
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Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico; Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps; Cell Press; Biophysical Journal; 90; 1; 12-2006; 7-9 0006-3495 CONICET Digital CONICET |
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eng |
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eng |
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