Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps

Autores
Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. University of Illinois at Urbana; Estados Unidos
Fil: Gelfand, Vladimir I.. Northwestern University; Estados Unidos
Fil: Aguirre, Ivanna Verónica. Northwestern University; Estados Unidos
Fil: Gratton, Enrico. University of Illinois at Urbana; Estados Unidos
Materia
myosin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100329
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm stepsLevi, ValeriaGelfand, Vladimir I.Aguirre, Ivanna VerónicaGratton, Enricomyosinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. University of Illinois at Urbana; Estados UnidosFil: Gelfand, Vladimir I.. Northwestern University; Estados UnidosFil: Aguirre, Ivanna Verónica. Northwestern University; Estados UnidosFil: Gratton, Enrico. University of Illinois at Urbana; Estados UnidosCell Press2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100329Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico; Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps; Cell Press; Biophysical Journal; 90; 1; 12-2006; 7-90006-3495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cell.com/biophysj/fulltext/S0006-3495(06)72184-1info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367043/info:eu-repo/semantics/altIdentifier/doi/10.1529/biophysj.105.075556info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:25Zoai:ri.conicet.gov.ar:11336/100329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:25.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
title Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
spellingShingle Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
Levi, Valeria
myosin
title_short Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
title_full Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
title_fullStr Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
title_full_unstemmed Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
title_sort Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps
dc.creator.none.fl_str_mv Levi, Valeria
Gelfand, Vladimir I.
Aguirre, Ivanna Verónica
Gratton, Enrico
author Levi, Valeria
author_facet Levi, Valeria
Gelfand, Vladimir I.
Aguirre, Ivanna Verónica
Gratton, Enrico
author_role author
author2 Gelfand, Vladimir I.
Aguirre, Ivanna Verónica
Gratton, Enrico
author2_role author
author
author
dc.subject.none.fl_str_mv myosin
topic myosin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. University of Illinois at Urbana; Estados Unidos
Fil: Gelfand, Vladimir I.. Northwestern University; Estados Unidos
Fil: Aguirre, Ivanna Verónica. Northwestern University; Estados Unidos
Fil: Gratton, Enrico. University of Illinois at Urbana; Estados Unidos
description We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly the melanosomes' motion.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100329
Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico; Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps; Cell Press; Biophysical Journal; 90; 1; 12-2006; 7-9
0006-3495
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100329
identifier_str_mv Levi, Valeria; Gelfand, Vladimir I.; Aguirre, Ivanna Verónica; Gratton, Enrico; Melanosomes transported by myosin-V in xenopus melanophores perform slow 35 nm steps; Cell Press; Biophysical Journal; 90; 1; 12-2006; 7-9
0006-3495
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/biophysj/fulltext/S0006-3495(06)72184-1
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367043/
info:eu-repo/semantics/altIdentifier/doi/10.1529/biophysj.105.075556
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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