Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii

Autores
Paggi, Roberto Alejandro; Madrid, Enrique Alberto; D'alessandro, Celeste Paola; de Castro, Rosana Esther
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and Results: Nep mRNA and extracellular protease activity were measured by RT-PCR and azocaseinolytic activity determination, respectively. Increased abundance in Nep mRNA was observed in Nab. magadii cells with culture age, which correlated with accumulation of extracellular protease activity. Moreover, a ‘stationary phase behavior’ on synthesis of Nep was evidenced in low-density cultures incubated with stationary phase medium. Conclusions: nep gene expression is up-regulated during the transition to the stationary phase in response to "factors" (metabolite and ⁄ or regulatory molecule) occurring in high-density cultures of Nab. magadii. Although the identity of these molecules remains to be determined, preliminary evidence suggests that they are hydrophobic and stable in high salt and high pH values (3Æ5 mol l)1 NaCl, pH 10). Significance and impact of study: This study contributes to gain insight into the regulation of haloarchaeal protease biosynthesis, facilitating the large-scale production of this extremozyme for basic studies or potential applications.
Fil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Madrid, Enrique Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: D'alessandro, Celeste Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
NATRIALBA MAGADII
ARCHAEA
HALOLYSIN-LIKE PROTEASE
GENE REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104252
Acceder
id CONICETDig_aefafbc9bdc5a5c466bd48c005cd3b0b
oai_identifier_str oai:ri.conicet.gov.ar:11336/104252
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadiiPaggi, Roberto AlejandroMadrid, Enrique AlbertoD'alessandro, Celeste Paolade Castro, Rosana EstherNATRIALBA MAGADIIARCHAEAHALOLYSIN-LIKE PROTEASEGENE REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and Results: Nep mRNA and extracellular protease activity were measured by RT-PCR and azocaseinolytic activity determination, respectively. Increased abundance in Nep mRNA was observed in Nab. magadii cells with culture age, which correlated with accumulation of extracellular protease activity. Moreover, a ‘stationary phase behavior’ on synthesis of Nep was evidenced in low-density cultures incubated with stationary phase medium. Conclusions: nep gene expression is up-regulated during the transition to the stationary phase in response to "factors" (metabolite and ⁄ or regulatory molecule) occurring in high-density cultures of Nab. magadii. Although the identity of these molecules remains to be determined, preliminary evidence suggests that they are hydrophobic and stable in high salt and high pH values (3Æ5 mol l)1 NaCl, pH 10). Significance and impact of study: This study contributes to gain insight into the regulation of haloarchaeal protease biosynthesis, facilitating the large-scale production of this extremozyme for basic studies or potential applications.Fil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Madrid, Enrique Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: D'alessandro, Celeste Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaWiley Blackwell Publishing, Inc2010-06-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104252Paggi, Roberto Alejandro; Madrid, Enrique Alberto; D'alessandro, Celeste Paola; de Castro, Rosana Esther; Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 9-6-2010; 36-410266-8254CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2010.02855.xinfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2010.02855.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:45:41Zoai:ri.conicet.gov.ar:11336/104252instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:45:42.254CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
title Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
spellingShingle Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
Paggi, Roberto Alejandro
NATRIALBA MAGADII
ARCHAEA
HALOLYSIN-LIKE PROTEASE
GENE REGULATION
title_short Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
title_full Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
title_fullStr Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
title_full_unstemmed Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
title_sort Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
dc.creator.none.fl_str_mv Paggi, Roberto Alejandro
Madrid, Enrique Alberto
D'alessandro, Celeste Paola
de Castro, Rosana Esther
author Paggi, Roberto Alejandro
author_facet Paggi, Roberto Alejandro
Madrid, Enrique Alberto
D'alessandro, Celeste Paola
de Castro, Rosana Esther
author_role author
author2 Madrid, Enrique Alberto
D'alessandro, Celeste Paola
de Castro, Rosana Esther
author2_role author
author
author
dc.subject.none.fl_str_mv NATRIALBA MAGADII
ARCHAEA
HALOLYSIN-LIKE PROTEASE
GENE REGULATION
topic NATRIALBA MAGADII
ARCHAEA
HALOLYSIN-LIKE PROTEASE
GENE REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and Results: Nep mRNA and extracellular protease activity were measured by RT-PCR and azocaseinolytic activity determination, respectively. Increased abundance in Nep mRNA was observed in Nab. magadii cells with culture age, which correlated with accumulation of extracellular protease activity. Moreover, a ‘stationary phase behavior’ on synthesis of Nep was evidenced in low-density cultures incubated with stationary phase medium. Conclusions: nep gene expression is up-regulated during the transition to the stationary phase in response to "factors" (metabolite and ⁄ or regulatory molecule) occurring in high-density cultures of Nab. magadii. Although the identity of these molecules remains to be determined, preliminary evidence suggests that they are hydrophobic and stable in high salt and high pH values (3Æ5 mol l)1 NaCl, pH 10). Significance and impact of study: This study contributes to gain insight into the regulation of haloarchaeal protease biosynthesis, facilitating the large-scale production of this extremozyme for basic studies or potential applications.
Fil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Madrid, Enrique Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: D'alessandro, Celeste Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and Results: Nep mRNA and extracellular protease activity were measured by RT-PCR and azocaseinolytic activity determination, respectively. Increased abundance in Nep mRNA was observed in Nab. magadii cells with culture age, which correlated with accumulation of extracellular protease activity. Moreover, a ‘stationary phase behavior’ on synthesis of Nep was evidenced in low-density cultures incubated with stationary phase medium. Conclusions: nep gene expression is up-regulated during the transition to the stationary phase in response to "factors" (metabolite and ⁄ or regulatory molecule) occurring in high-density cultures of Nab. magadii. Although the identity of these molecules remains to be determined, preliminary evidence suggests that they are hydrophobic and stable in high salt and high pH values (3Æ5 mol l)1 NaCl, pH 10). Significance and impact of study: This study contributes to gain insight into the regulation of haloarchaeal protease biosynthesis, facilitating the large-scale production of this extremozyme for basic studies or potential applications.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104252
Paggi, Roberto Alejandro; Madrid, Enrique Alberto; D'alessandro, Celeste Paola; de Castro, Rosana Esther; Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 9-6-2010; 36-41
0266-8254
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104252
identifier_str_mv Paggi, Roberto Alejandro; Madrid, Enrique Alberto; D'alessandro, Celeste Paola; de Castro, Rosana Esther; Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 9-6-2010; 36-41
0266-8254
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2010.02855.x
info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2010.02855.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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