Activation and Expansion of Human T-Cells Using Microfluidic Devices

Autores
Peñaherrera Pazmiño, Ana Belén; Rosero, Gustavo; Ruarte, Dario; Pinter, Julia; Vizuete, Karla; Perez, Maximiliano; Follo, Marie; Lerner, Betiana; Mertelsmann, Roland
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Treatment of cancer patients with autologous T-cells expressing a chimeric antigen receptor (CAR) is one of the most promising therapeutic modalities for hematological malignancy treatment. For this treatment, primary T-cell expansion is needed. Microfluidic technologies can be used to better understand T-cell activation and proliferation. Microfluidics have had a meaningful impact in the way experimental biology and biomedical research are approached in general. Furthermore, microfluidic technology allows the generation of large amounts of data and enables the use of image processing for analysis. However, one of the major technical hurdles involved in growing suspension cells under microfluidic conditions is their immobilization, to avoid washing them out of the microfluidic chip during medium renewal. In this work, we use a multilevel microfluidic chip to successfully capture and immobilize suspension cells. Jurkat cells and T-cells are isolated through traps to microscopically track their development and proliferation after activation over a period of 8 days. The T-cell area of four independent microchannels was compared and there is no statistically significant difference between them (ANOVA p-value = 0.976). These multilevel microfluidic chips provide a new method of studying T-cell activation.
Fil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosero, Gustavo. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Fil: Ruarte, Dario. Albert Ludwigs University of Freiburg; Alemania
Fil: Pinter, Julia. Albert Ludwigs University of Freiburg; Alemania
Fil: Vizuete, Karla. Universidad de Las Fuerzas Armadas; Ecuador
Fil: Perez, Maximiliano. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Florida International University; Estados Unidos
Fil: Follo, Marie. Albert Ludwigs University of Freiburg; Alemania
Fil: Lerner, Betiana. Florida International University; Estados Unidos. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mertelsmann, Roland. Albert Ludwigs University of Freiburg; Alemania
Materia
MICROFLUÍDIC
SUSPENSION CELLS
T-CELL EXPANSION
HEALTH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266002
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activation and Expansion of Human T-Cells Using Microfluidic DevicesPeñaherrera Pazmiño, Ana BelénRosero, GustavoRuarte, DarioPinter, JuliaVizuete, KarlaPerez, MaximilianoFollo, MarieLerner, BetianaMertelsmann, RolandMICROFLUÍDICSUSPENSION CELLST-CELL EXPANSIONHEALTHhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Treatment of cancer patients with autologous T-cells expressing a chimeric antigen receptor (CAR) is one of the most promising therapeutic modalities for hematological malignancy treatment. For this treatment, primary T-cell expansion is needed. Microfluidic technologies can be used to better understand T-cell activation and proliferation. Microfluidics have had a meaningful impact in the way experimental biology and biomedical research are approached in general. Furthermore, microfluidic technology allows the generation of large amounts of data and enables the use of image processing for analysis. However, one of the major technical hurdles involved in growing suspension cells under microfluidic conditions is their immobilization, to avoid washing them out of the microfluidic chip during medium renewal. In this work, we use a multilevel microfluidic chip to successfully capture and immobilize suspension cells. Jurkat cells and T-cells are isolated through traps to microscopically track their development and proliferation after activation over a period of 8 days. The T-cell area of four independent microchannels was compared and there is no statistically significant difference between them (ANOVA p-value = 0.976). These multilevel microfluidic chips provide a new method of studying T-cell activation.Fil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosero, Gustavo. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; ArgentinaFil: Ruarte, Dario. Albert Ludwigs University of Freiburg; AlemaniaFil: Pinter, Julia. Albert Ludwigs University of Freiburg; AlemaniaFil: Vizuete, Karla. Universidad de Las Fuerzas Armadas; EcuadorFil: Perez, Maximiliano. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Florida International University; Estados UnidosFil: Follo, Marie. Albert Ludwigs University of Freiburg; AlemaniaFil: Lerner, Betiana. Florida International University; Estados Unidos. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mertelsmann, Roland. Albert Ludwigs University of Freiburg; AlemaniaMDPI2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266002Peñaherrera Pazmiño, Ana Belén; Rosero, Gustavo; Ruarte, Dario; Pinter, Julia; Vizuete, Karla; et al.; Activation and Expansion of Human T-Cells Using Microfluidic Devices; MDPI; Biosensors; 15; 5; 4-2025; 1-172079-6374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/15/5/270info:eu-repo/semantics/altIdentifier/doi/10.3390/bios15050270info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:41Zoai:ri.conicet.gov.ar:11336/266002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:41.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activation and Expansion of Human T-Cells Using Microfluidic Devices
title Activation and Expansion of Human T-Cells Using Microfluidic Devices
spellingShingle Activation and Expansion of Human T-Cells Using Microfluidic Devices
Peñaherrera Pazmiño, Ana Belén
MICROFLUÍDIC
SUSPENSION CELLS
T-CELL EXPANSION
HEALTH
title_short Activation and Expansion of Human T-Cells Using Microfluidic Devices
title_full Activation and Expansion of Human T-Cells Using Microfluidic Devices
title_fullStr Activation and Expansion of Human T-Cells Using Microfluidic Devices
title_full_unstemmed Activation and Expansion of Human T-Cells Using Microfluidic Devices
title_sort Activation and Expansion of Human T-Cells Using Microfluidic Devices
dc.creator.none.fl_str_mv Peñaherrera Pazmiño, Ana Belén
Rosero, Gustavo
Ruarte, Dario
Pinter, Julia
Vizuete, Karla
Perez, Maximiliano
Follo, Marie
Lerner, Betiana
Mertelsmann, Roland
author Peñaherrera Pazmiño, Ana Belén
author_facet Peñaherrera Pazmiño, Ana Belén
Rosero, Gustavo
Ruarte, Dario
Pinter, Julia
Vizuete, Karla
Perez, Maximiliano
Follo, Marie
Lerner, Betiana
Mertelsmann, Roland
author_role author
author2 Rosero, Gustavo
Ruarte, Dario
Pinter, Julia
Vizuete, Karla
Perez, Maximiliano
Follo, Marie
Lerner, Betiana
Mertelsmann, Roland
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROFLUÍDIC
SUSPENSION CELLS
T-CELL EXPANSION
HEALTH
topic MICROFLUÍDIC
SUSPENSION CELLS
T-CELL EXPANSION
HEALTH
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Treatment of cancer patients with autologous T-cells expressing a chimeric antigen receptor (CAR) is one of the most promising therapeutic modalities for hematological malignancy treatment. For this treatment, primary T-cell expansion is needed. Microfluidic technologies can be used to better understand T-cell activation and proliferation. Microfluidics have had a meaningful impact in the way experimental biology and biomedical research are approached in general. Furthermore, microfluidic technology allows the generation of large amounts of data and enables the use of image processing for analysis. However, one of the major technical hurdles involved in growing suspension cells under microfluidic conditions is their immobilization, to avoid washing them out of the microfluidic chip during medium renewal. In this work, we use a multilevel microfluidic chip to successfully capture and immobilize suspension cells. Jurkat cells and T-cells are isolated through traps to microscopically track their development and proliferation after activation over a period of 8 days. The T-cell area of four independent microchannels was compared and there is no statistically significant difference between them (ANOVA p-value = 0.976). These multilevel microfluidic chips provide a new method of studying T-cell activation.
Fil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosero, Gustavo. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Fil: Ruarte, Dario. Albert Ludwigs University of Freiburg; Alemania
Fil: Pinter, Julia. Albert Ludwigs University of Freiburg; Alemania
Fil: Vizuete, Karla. Universidad de Las Fuerzas Armadas; Ecuador
Fil: Perez, Maximiliano. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Florida International University; Estados Unidos
Fil: Follo, Marie. Albert Ludwigs University of Freiburg; Alemania
Fil: Lerner, Betiana. Florida International University; Estados Unidos. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mertelsmann, Roland. Albert Ludwigs University of Freiburg; Alemania
description Treatment of cancer patients with autologous T-cells expressing a chimeric antigen receptor (CAR) is one of the most promising therapeutic modalities for hematological malignancy treatment. For this treatment, primary T-cell expansion is needed. Microfluidic technologies can be used to better understand T-cell activation and proliferation. Microfluidics have had a meaningful impact in the way experimental biology and biomedical research are approached in general. Furthermore, microfluidic technology allows the generation of large amounts of data and enables the use of image processing for analysis. However, one of the major technical hurdles involved in growing suspension cells under microfluidic conditions is their immobilization, to avoid washing them out of the microfluidic chip during medium renewal. In this work, we use a multilevel microfluidic chip to successfully capture and immobilize suspension cells. Jurkat cells and T-cells are isolated through traps to microscopically track their development and proliferation after activation over a period of 8 days. The T-cell area of four independent microchannels was compared and there is no statistically significant difference between them (ANOVA p-value = 0.976). These multilevel microfluidic chips provide a new method of studying T-cell activation.
publishDate 2025
dc.date.none.fl_str_mv 2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266002
Peñaherrera Pazmiño, Ana Belén; Rosero, Gustavo; Ruarte, Dario; Pinter, Julia; Vizuete, Karla; et al.; Activation and Expansion of Human T-Cells Using Microfluidic Devices; MDPI; Biosensors; 15; 5; 4-2025; 1-17
2079-6374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266002
identifier_str_mv Peñaherrera Pazmiño, Ana Belén; Rosero, Gustavo; Ruarte, Dario; Pinter, Julia; Vizuete, Karla; et al.; Activation and Expansion of Human T-Cells Using Microfluidic Devices; MDPI; Biosensors; 15; 5; 4-2025; 1-17
2079-6374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/15/5/270
info:eu-repo/semantics/altIdentifier/doi/10.3390/bios15050270
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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