Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker...
- Autores
- Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome.
Fil: Jalili, Cyrus. Kermanshah University Of Medical Sciences; Irán
Fil: Akbari, Vali. Kermanshah University Of Medical Sciences; Irán
Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán - Materia
-
BIVALIRUDIN
DETERMINATION
INHIBITION
MYELOPEROXIDASE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/164792
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Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndromeJalili, CyrusAkbari, ValiKhodarahmi, RezaGoicoechea, Hector CasimiroJalalvand, Ali R.BIVALIRUDINDETERMINATIONINHIBITIONMYELOPEROXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome.Fil: Jalili, Cyrus. Kermanshah University Of Medical Sciences; IránFil: Akbari, Vali. Kermanshah University Of Medical Sciences; IránFil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; IránFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; IránElsevier Science2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164792Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome; Elsevier Science; Microchemical Journal; 166; 7-2021; 1-300026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0026265X21002964info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2021.106212info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:48Zoai:ri.conicet.gov.ar:11336/164792instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:49.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
title |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
spellingShingle |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome Jalili, Cyrus BIVALIRUDIN DETERMINATION INHIBITION MYELOPEROXIDASE |
title_short |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
title_full |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
title_fullStr |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
title_full_unstemmed |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
title_sort |
Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome |
dc.creator.none.fl_str_mv |
Jalili, Cyrus Akbari, Vali Khodarahmi, Reza Goicoechea, Hector Casimiro Jalalvand, Ali R. |
author |
Jalili, Cyrus |
author_facet |
Jalili, Cyrus Akbari, Vali Khodarahmi, Reza Goicoechea, Hector Casimiro Jalalvand, Ali R. |
author_role |
author |
author2 |
Akbari, Vali Khodarahmi, Reza Goicoechea, Hector Casimiro Jalalvand, Ali R. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
BIVALIRUDIN DETERMINATION INHIBITION MYELOPEROXIDASE |
topic |
BIVALIRUDIN DETERMINATION INHIBITION MYELOPEROXIDASE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome. Fil: Jalili, Cyrus. Kermanshah University Of Medical Sciences; Irán Fil: Akbari, Vali. Kermanshah University Of Medical Sciences; Irán Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán |
description |
In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/164792 Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome; Elsevier Science; Microchemical Journal; 166; 7-2021; 1-30 0026-265X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/164792 |
identifier_str_mv |
Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome; Elsevier Science; Microchemical Journal; 166; 7-2021; 1-30 0026-265X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0026265X21002964 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2021.106212 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613227291344896 |
score |
13.070432 |