Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker...

Autores
Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome.
Fil: Jalili, Cyrus. Kermanshah University Of Medical Sciences; Irán
Fil: Akbari, Vali. Kermanshah University Of Medical Sciences; Irán
Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
Materia
BIVALIRUDIN
DETERMINATION
INHIBITION
MYELOPEROXIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164792

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network_name_str CONICET Digital (CONICET)
spelling Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndromeJalili, CyrusAkbari, ValiKhodarahmi, RezaGoicoechea, Hector CasimiroJalalvand, Ali R.BIVALIRUDINDETERMINATIONINHIBITIONMYELOPEROXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome.Fil: Jalili, Cyrus. Kermanshah University Of Medical Sciences; IránFil: Akbari, Vali. Kermanshah University Of Medical Sciences; IránFil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; IránFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; IránElsevier Science2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164792Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome; Elsevier Science; Microchemical Journal; 166; 7-2021; 1-300026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0026265X21002964info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2021.106212info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:48Zoai:ri.conicet.gov.ar:11336/164792instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:49.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
title Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
spellingShingle Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
Jalili, Cyrus
BIVALIRUDIN
DETERMINATION
INHIBITION
MYELOPEROXIDASE
title_short Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
title_full Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
title_fullStr Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
title_full_unstemmed Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
title_sort Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome
dc.creator.none.fl_str_mv Jalili, Cyrus
Akbari, Vali
Khodarahmi, Reza
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author Jalili, Cyrus
author_facet Jalili, Cyrus
Akbari, Vali
Khodarahmi, Reza
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author_role author
author2 Akbari, Vali
Khodarahmi, Reza
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIVALIRUDIN
DETERMINATION
INHIBITION
MYELOPEROXIDASE
topic BIVALIRUDIN
DETERMINATION
INHIBITION
MYELOPEROXIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome.
Fil: Jalili, Cyrus. Kermanshah University Of Medical Sciences; Irán
Fil: Akbari, Vali. Kermanshah University Of Medical Sciences; Irán
Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
description In this paper, we are going to report results of one of our projects in which we have investigated binding and inhibition of myeloperoxidase (MO) by bivalirudin (BV) with the help of electrochemical and spectroscopic techniques assisted by chemometric methods. Different electrochemical and spectroscopic data were combined or individually used to justify binding and inhibition of the MO by BV. Hard and soft modeling chemometric methods were used to the analysis of experimental data to extract concentration profiles, pure instrumental signal profiles and binding constant value (Kb). Results of conventional and chemometric analyses confirmed a relatively strong binding of BV with MO which inhibited it. Concentration and pure instrumental signal profiles revealed interaction and binding of BV with MO and formation of one complex species. Inhibition of the MO by BV helped us to exploit a quantitative concept for electrochemical determination of the MO based on its binding with BV. Determination of the MO was performed by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) and the results of the EIS were better than DPV which can be suggested for the analysis of serum samples with the aim of evaluation of patients with acute coronary syndrome.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164792
Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome; Elsevier Science; Microchemical Journal; 166; 7-2021; 1-30
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164792
identifier_str_mv Jalili, Cyrus; Akbari, Vali; Khodarahmi, Reza; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Electrochemical-spectroscopic-chemometric investigation of binding and inhibition of myeloperoxidase by bivalirudin: application to determination of myeloperoxidase as a biomarker for acute coronary syndrome; Elsevier Science; Microchemical Journal; 166; 7-2021; 1-30
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0026265X21002964
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2021.106212
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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