Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate

Autores
Jalalvand, Ali R.; Mohammadi, Ghobad; Najafi, Farid; Sadeghi, Ehsan; Bahrami, Gholamreza; Rostamzad, Arman; Sadrjavadi, Komail; Azadi, Ehsan; Adibi, Hadi; Goicoechea, Hector Casimiro; Abbasi, Naser; Ghaneialvar, Hori
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
By this article, we are going to report results of one of works which has been performed on investigation of the binding and inhibition of catalase (CAT) by ascorbate (ASC). To achieve this goal, different electrochemical experiments have been performed and their data have been analyzed by conventional and chemometric methods. Conventional methods were including direct analysis of the electrochemical data by observation of them and using simple mathematical equations while chemometric analyses of the electrochemical data helped us to obtain more information which completed the previous information and gave us a new insight to the binding of the ASC with CAT. The next step of our study was devoted to the investigation of the binding of ASC with CAT by molecular docking methods which gave us new information about binding and inhibition of the CAT by ASC. All the steps gave specific information which not only confirmed each other but also gave new information which helped us to better understanding the mechanism of the binding and inhibition of the CAT by ASC. Finally, based on inhibition of the CAT by ASC, we have developed a novel impedimetric method for determination of the CAT.
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
Fil: Mohammadi, Ghobad. Kermanshah University Of Medical Sciences; Irán
Fil: Najafi, Farid. Kermanshah University Of Medical Sciences; Irán
Fil: Sadeghi, Ehsan. Kermanshah University Of Medical Sciences; Irán
Fil: Bahrami, Gholamreza. Kermanshah University Of Medical Sciences; Irán
Fil: Rostamzad, Arman. Kermanshah University Of Medical Sciences; Irán
Fil: Sadrjavadi, Komail. Kermanshah University Of Medical Sciences; Irán
Fil: Azadi, Ehsan. Kermanshah University Of Medical Sciences; Irán
Fil: Adibi, Hadi. Kermanshah University Of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Abbasi, Naser. Kermanshah University Of Medical Sciences; Irán
Fil: Ghaneialvar, Hori. Kermanshah University Of Medical Sciences; Irán
Materia
ASCORBATE
CATALASE
INHIBITION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/152014
Acceder
id CONICETDig_0fa7d424e850491e910278d05cef8e65
oai_identifier_str oai:ri.conicet.gov.ar:11336/152014
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbateJalalvand, Ali R.Mohammadi, GhobadNajafi, FaridSadeghi, EhsanBahrami, GholamrezaRostamzad, ArmanSadrjavadi, KomailAzadi, EhsanAdibi, HadiGoicoechea, Hector CasimiroAbbasi, NaserGhaneialvar, HoriASCORBATECATALASEINHIBITIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1By this article, we are going to report results of one of works which has been performed on investigation of the binding and inhibition of catalase (CAT) by ascorbate (ASC). To achieve this goal, different electrochemical experiments have been performed and their data have been analyzed by conventional and chemometric methods. Conventional methods were including direct analysis of the electrochemical data by observation of them and using simple mathematical equations while chemometric analyses of the electrochemical data helped us to obtain more information which completed the previous information and gave us a new insight to the binding of the ASC with CAT. The next step of our study was devoted to the investigation of the binding of ASC with CAT by molecular docking methods which gave us new information about binding and inhibition of the CAT by ASC. All the steps gave specific information which not only confirmed each other but also gave new information which helped us to better understanding the mechanism of the binding and inhibition of the CAT by ASC. Finally, based on inhibition of the CAT by ASC, we have developed a novel impedimetric method for determination of the CAT.Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; IránFil: Mohammadi, Ghobad. Kermanshah University Of Medical Sciences; IránFil: Najafi, Farid. Kermanshah University Of Medical Sciences; IránFil: Sadeghi, Ehsan. Kermanshah University Of Medical Sciences; IránFil: Bahrami, Gholamreza. Kermanshah University Of Medical Sciences; IránFil: Rostamzad, Arman. Kermanshah University Of Medical Sciences; IránFil: Sadrjavadi, Komail. Kermanshah University Of Medical Sciences; IránFil: Azadi, Ehsan. Kermanshah University Of Medical Sciences; IránFil: Adibi, Hadi. Kermanshah University Of Medical Sciences; IránFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Abbasi, Naser. Kermanshah University Of Medical Sciences; IránFil: Ghaneialvar, Hori. Kermanshah University Of Medical Sciences; IránElsevier2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152014Jalalvand, Ali R.; Mohammadi, Ghobad; Najafi, Farid; Sadeghi, Ehsan; Bahrami, Gholamreza; et al.; Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate; Elsevier; Sensing and Bio-Sensing Research; 30; 12-2020; 1-192214-1804CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2214180420302087info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbsr.2020.100383info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:23Zoai:ri.conicet.gov.ar:11336/152014instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:23.382CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
title Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
spellingShingle Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
Jalalvand, Ali R.
ASCORBATE
CATALASE
INHIBITION
title_short Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
title_full Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
title_fullStr Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
title_full_unstemmed Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
title_sort Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate
dc.creator.none.fl_str_mv Jalalvand, Ali R.
Mohammadi, Ghobad
Najafi, Farid
Sadeghi, Ehsan
Bahrami, Gholamreza
Rostamzad, Arman
Sadrjavadi, Komail
Azadi, Ehsan
Adibi, Hadi
Goicoechea, Hector Casimiro
Abbasi, Naser
Ghaneialvar, Hori
author Jalalvand, Ali R.
author_facet Jalalvand, Ali R.
Mohammadi, Ghobad
Najafi, Farid
Sadeghi, Ehsan
Bahrami, Gholamreza
Rostamzad, Arman
Sadrjavadi, Komail
Azadi, Ehsan
Adibi, Hadi
Goicoechea, Hector Casimiro
Abbasi, Naser
Ghaneialvar, Hori
author_role author
author2 Mohammadi, Ghobad
Najafi, Farid
Sadeghi, Ehsan
Bahrami, Gholamreza
Rostamzad, Arman
Sadrjavadi, Komail
Azadi, Ehsan
Adibi, Hadi
Goicoechea, Hector Casimiro
Abbasi, Naser
Ghaneialvar, Hori
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASCORBATE
CATALASE
INHIBITION
topic ASCORBATE
CATALASE
INHIBITION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv By this article, we are going to report results of one of works which has been performed on investigation of the binding and inhibition of catalase (CAT) by ascorbate (ASC). To achieve this goal, different electrochemical experiments have been performed and their data have been analyzed by conventional and chemometric methods. Conventional methods were including direct analysis of the electrochemical data by observation of them and using simple mathematical equations while chemometric analyses of the electrochemical data helped us to obtain more information which completed the previous information and gave us a new insight to the binding of the ASC with CAT. The next step of our study was devoted to the investigation of the binding of ASC with CAT by molecular docking methods which gave us new information about binding and inhibition of the CAT by ASC. All the steps gave specific information which not only confirmed each other but also gave new information which helped us to better understanding the mechanism of the binding and inhibition of the CAT by ASC. Finally, based on inhibition of the CAT by ASC, we have developed a novel impedimetric method for determination of the CAT.
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
Fil: Mohammadi, Ghobad. Kermanshah University Of Medical Sciences; Irán
Fil: Najafi, Farid. Kermanshah University Of Medical Sciences; Irán
Fil: Sadeghi, Ehsan. Kermanshah University Of Medical Sciences; Irán
Fil: Bahrami, Gholamreza. Kermanshah University Of Medical Sciences; Irán
Fil: Rostamzad, Arman. Kermanshah University Of Medical Sciences; Irán
Fil: Sadrjavadi, Komail. Kermanshah University Of Medical Sciences; Irán
Fil: Azadi, Ehsan. Kermanshah University Of Medical Sciences; Irán
Fil: Adibi, Hadi. Kermanshah University Of Medical Sciences; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Abbasi, Naser. Kermanshah University Of Medical Sciences; Irán
Fil: Ghaneialvar, Hori. Kermanshah University Of Medical Sciences; Irán
description By this article, we are going to report results of one of works which has been performed on investigation of the binding and inhibition of catalase (CAT) by ascorbate (ASC). To achieve this goal, different electrochemical experiments have been performed and their data have been analyzed by conventional and chemometric methods. Conventional methods were including direct analysis of the electrochemical data by observation of them and using simple mathematical equations while chemometric analyses of the electrochemical data helped us to obtain more information which completed the previous information and gave us a new insight to the binding of the ASC with CAT. The next step of our study was devoted to the investigation of the binding of ASC with CAT by molecular docking methods which gave us new information about binding and inhibition of the CAT by ASC. All the steps gave specific information which not only confirmed each other but also gave new information which helped us to better understanding the mechanism of the binding and inhibition of the CAT by ASC. Finally, based on inhibition of the CAT by ASC, we have developed a novel impedimetric method for determination of the CAT.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152014
Jalalvand, Ali R.; Mohammadi, Ghobad; Najafi, Farid; Sadeghi, Ehsan; Bahrami, Gholamreza; et al.; Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate; Elsevier; Sensing and Bio-Sensing Research; 30; 12-2020; 1-19
2214-1804
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152014
identifier_str_mv Jalalvand, Ali R.; Mohammadi, Ghobad; Najafi, Farid; Sadeghi, Ehsan; Bahrami, Gholamreza; et al.; Developing an interesting electrochemical biosensing system from an enzyme inhibition study: Binding, inhibition and determination of catalase by ascorbate; Elsevier; Sensing and Bio-Sensing Research; 30; 12-2020; 1-19
2214-1804
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2214180420302087
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbsr.2020.100383
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614444491997184
score 13.070432

Publicaciones similares