Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees

Autores
Pimentel Betancurt, Diana Carolina; Tonello, Natalia Veronica; Padilla Alvarez, Francisco; Paletti Rovey, María Fernanda; Oliva, María M.; Marioli, Juan Miguel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To evaluate the antimicrobial activity of the hexanic extract (HE) of Achyrocline satureioides on Paenibacillus larvae - a gram-positive spore-forming bacillus that affects the larval stage and causes American Foulbrood (AFB) - and its oral and contact toxicity on larvae and adult honey bees. A. satureioides plants were collected in Santa Monica (32° 05’ 29” S, 64° 36’ 54” W, Córdoba. Argentina). The larvae and adults of Apis mellifera were obtained from the experimental apiary of the University of Córdoba, Spain. P. larvae 9 was previously isolated and identified in the Laboratory of General Microbiology (Dept. of Microbiology, National University of Río Cuarto, Argentina). The HE was obtained by liquid-liquid extraction. The minimum inhibitory concentration (MIC) of HE was determined by a microdilution method. This concentration and 2 ½ MIC were used for in vitro toxicity tests. Oral toxicity was tested on larvae, feeding them with both concentrations of the HE, while on adult bees the HE was spread to determine contact toxicity. The HE showed antimicrobial activity, the MIC obtained was 0.4 μg/mL. The HE presented very low toxicity at the MIC and 2 ½ MIC, with survival percentages to be around 95% for adult bees and larvae. The results show that this extract could be used for the development of an alternative product for a safe and effective treatment of AFB.
Fil: Pimentel Betancurt, Diana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Tonello, Natalia Veronica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Padilla Alvarez, Francisco. Universidad Nacional de Río Cuarto; Argentina
Fil: Paletti Rovey, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Oliva, María M.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
EXTRACTO HEXANICO
LOQUE AMERICANA
LARVAS
Achyrocline satureioides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/172179
Acceder
id CONICETDig_4b5ed6431d4418e6be93d5cf3951bb4d
oai_identifier_str oai:ri.conicet.gov.ar:11336/172179
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey beesPimentel Betancurt, Diana CarolinaTonello, Natalia VeronicaPadilla Alvarez, FranciscoPaletti Rovey, María FernandaOliva, María M.Marioli, Juan MiguelEXTRACTO HEXANICOLOQUE AMERICANALARVASAchyrocline satureioideshttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1To evaluate the antimicrobial activity of the hexanic extract (HE) of Achyrocline satureioides on Paenibacillus larvae - a gram-positive spore-forming bacillus that affects the larval stage and causes American Foulbrood (AFB) - and its oral and contact toxicity on larvae and adult honey bees. A. satureioides plants were collected in Santa Monica (32° 05’ 29” S, 64° 36’ 54” W, Córdoba. Argentina). The larvae and adults of Apis mellifera were obtained from the experimental apiary of the University of Córdoba, Spain. P. larvae 9 was previously isolated and identified in the Laboratory of General Microbiology (Dept. of Microbiology, National University of Río Cuarto, Argentina). The HE was obtained by liquid-liquid extraction. The minimum inhibitory concentration (MIC) of HE was determined by a microdilution method. This concentration and 2 ½ MIC were used for in vitro toxicity tests. Oral toxicity was tested on larvae, feeding them with both concentrations of the HE, while on adult bees the HE was spread to determine contact toxicity. The HE showed antimicrobial activity, the MIC obtained was 0.4 μg/mL. The HE presented very low toxicity at the MIC and 2 ½ MIC, with survival percentages to be around 95% for adult bees and larvae. The results show that this extract could be used for the development of an alternative product for a safe and effective treatment of AFB.Fil: Pimentel Betancurt, Diana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Tonello, Natalia Veronica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Padilla Alvarez, Francisco. Universidad Nacional de Río Cuarto; ArgentinaFil: Paletti Rovey, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Oliva, María M.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaSpanish National Institute for Agriculture and Food Research and Technology2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/172179Pimentel Betancurt, Diana Carolina; Tonello, Natalia Veronica; Padilla Alvarez, Francisco; Paletti Rovey, María Fernanda; Oliva, María M.; et al.; Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal of Agricultural Research; 19; 3; 8-2021; 1-91695-971X2171-9292CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/16302info:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2021193-16302info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:51Zoai:ri.conicet.gov.ar:11336/172179instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:51.594CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
title Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
spellingShingle Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
Pimentel Betancurt, Diana Carolina
EXTRACTO HEXANICO
LOQUE AMERICANA
LARVAS
Achyrocline satureioides
title_short Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
title_full Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
title_fullStr Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
title_full_unstemmed Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
title_sort Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
dc.creator.none.fl_str_mv Pimentel Betancurt, Diana Carolina
Tonello, Natalia Veronica
Padilla Alvarez, Francisco
Paletti Rovey, María Fernanda
Oliva, María M.
Marioli, Juan Miguel
author Pimentel Betancurt, Diana Carolina
author_facet Pimentel Betancurt, Diana Carolina
Tonello, Natalia Veronica
Padilla Alvarez, Francisco
Paletti Rovey, María Fernanda
Oliva, María M.
Marioli, Juan Miguel
author_role author
author2 Tonello, Natalia Veronica
Padilla Alvarez, Francisco
Paletti Rovey, María Fernanda
Oliva, María M.
Marioli, Juan Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EXTRACTO HEXANICO
LOQUE AMERICANA
LARVAS
Achyrocline satureioides
topic EXTRACTO HEXANICO
LOQUE AMERICANA
LARVAS
Achyrocline satureioides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To evaluate the antimicrobial activity of the hexanic extract (HE) of Achyrocline satureioides on Paenibacillus larvae - a gram-positive spore-forming bacillus that affects the larval stage and causes American Foulbrood (AFB) - and its oral and contact toxicity on larvae and adult honey bees. A. satureioides plants were collected in Santa Monica (32° 05’ 29” S, 64° 36’ 54” W, Córdoba. Argentina). The larvae and adults of Apis mellifera were obtained from the experimental apiary of the University of Córdoba, Spain. P. larvae 9 was previously isolated and identified in the Laboratory of General Microbiology (Dept. of Microbiology, National University of Río Cuarto, Argentina). The HE was obtained by liquid-liquid extraction. The minimum inhibitory concentration (MIC) of HE was determined by a microdilution method. This concentration and 2 ½ MIC were used for in vitro toxicity tests. Oral toxicity was tested on larvae, feeding them with both concentrations of the HE, while on adult bees the HE was spread to determine contact toxicity. The HE showed antimicrobial activity, the MIC obtained was 0.4 μg/mL. The HE presented very low toxicity at the MIC and 2 ½ MIC, with survival percentages to be around 95% for adult bees and larvae. The results show that this extract could be used for the development of an alternative product for a safe and effective treatment of AFB.
Fil: Pimentel Betancurt, Diana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Tonello, Natalia Veronica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Padilla Alvarez, Francisco. Universidad Nacional de Río Cuarto; Argentina
Fil: Paletti Rovey, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Oliva, María M.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description To evaluate the antimicrobial activity of the hexanic extract (HE) of Achyrocline satureioides on Paenibacillus larvae - a gram-positive spore-forming bacillus that affects the larval stage and causes American Foulbrood (AFB) - and its oral and contact toxicity on larvae and adult honey bees. A. satureioides plants were collected in Santa Monica (32° 05’ 29” S, 64° 36’ 54” W, Córdoba. Argentina). The larvae and adults of Apis mellifera were obtained from the experimental apiary of the University of Córdoba, Spain. P. larvae 9 was previously isolated and identified in the Laboratory of General Microbiology (Dept. of Microbiology, National University of Río Cuarto, Argentina). The HE was obtained by liquid-liquid extraction. The minimum inhibitory concentration (MIC) of HE was determined by a microdilution method. This concentration and 2 ½ MIC were used for in vitro toxicity tests. Oral toxicity was tested on larvae, feeding them with both concentrations of the HE, while on adult bees the HE was spread to determine contact toxicity. The HE showed antimicrobial activity, the MIC obtained was 0.4 μg/mL. The HE presented very low toxicity at the MIC and 2 ½ MIC, with survival percentages to be around 95% for adult bees and larvae. The results show that this extract could be used for the development of an alternative product for a safe and effective treatment of AFB.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/172179
Pimentel Betancurt, Diana Carolina; Tonello, Natalia Veronica; Padilla Alvarez, Francisco; Paletti Rovey, María Fernanda; Oliva, María M.; et al.; Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal of Agricultural Research; 19; 3; 8-2021; 1-9
1695-971X
2171-9292
CONICET Digital
CONICET
url http://hdl.handle.net/11336/172179
identifier_str_mv Pimentel Betancurt, Diana Carolina; Tonello, Natalia Veronica; Padilla Alvarez, Francisco; Paletti Rovey, María Fernanda; Oliva, María M.; et al.; Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal of Agricultural Research; 19; 3; 8-2021; 1-9
1695-971X
2171-9292
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/16302
info:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2021193-16302
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269187133669376
score 13.13397

Publicaciones similares