Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice

Autores
Bucciarelli, Alejandro; Bras, Cristina Liliana; Gandini, Norberto Ariel; Guardiola, Florencia Magalí; Minetti, Alejandra; Skliar, Mario Ignacio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety.
Fil: Bucciarelli, Alejandro. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bras, Cristina Liliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Guardiola, Florencia Magalí. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Minetti, Alejandra. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Skliar, Mario Ignacio. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010
Córdoba
Argentina
Universidad Nacional de Cordoba
Universidad Nacional de Rosario
Materia
TOXICITY
SOLIDAGO CHILENSIS
SUBACUTE
EVALUATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243858

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spelling Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in miceBucciarelli, AlejandroBras, Cristina LilianaGandini, Norberto ArielGuardiola, Florencia MagalíMinetti, AlejandraSkliar, Mario IgnacioTOXICITYSOLIDAGO CHILENSISSUBACUTEEVALUATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety.Fil: Bucciarelli, Alejandro. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bras, Cristina Liliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Guardiola, Florencia Magalí. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Minetti, Alejandra. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Skliar, Mario Ignacio. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaPrimera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010CórdobaArgentinaUniversidad Nacional de CordobaUniversidad Nacional de RosarioUniversidad Nacional de Cordoba; Universidad Nacional de Rosario2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243858Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice; Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010; Córdoba; Argentina; 2010; 144-145CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ricifa.com.ar/wp-content/uploads/2021/09/Resumenes-2010.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:43Zoai:ri.conicet.gov.ar:11336/243858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:43.633CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
title Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
spellingShingle Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
Bucciarelli, Alejandro
TOXICITY
SOLIDAGO CHILENSIS
SUBACUTE
EVALUATION
title_short Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
title_full Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
title_fullStr Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
title_full_unstemmed Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
title_sort Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
dc.creator.none.fl_str_mv Bucciarelli, Alejandro
Bras, Cristina Liliana
Gandini, Norberto Ariel
Guardiola, Florencia Magalí
Minetti, Alejandra
Skliar, Mario Ignacio
author Bucciarelli, Alejandro
author_facet Bucciarelli, Alejandro
Bras, Cristina Liliana
Gandini, Norberto Ariel
Guardiola, Florencia Magalí
Minetti, Alejandra
Skliar, Mario Ignacio
author_role author
author2 Bras, Cristina Liliana
Gandini, Norberto Ariel
Guardiola, Florencia Magalí
Minetti, Alejandra
Skliar, Mario Ignacio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv TOXICITY
SOLIDAGO CHILENSIS
SUBACUTE
EVALUATION
topic TOXICITY
SOLIDAGO CHILENSIS
SUBACUTE
EVALUATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety.
Fil: Bucciarelli, Alejandro. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bras, Cristina Liliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Guardiola, Florencia Magalí. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Minetti, Alejandra. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Skliar, Mario Ignacio. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010
Córdoba
Argentina
Universidad Nacional de Cordoba
Universidad Nacional de Rosario
description Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety.
publishDate 2010
dc.date.none.fl_str_mv 2010
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243858
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice; Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010; Córdoba; Argentina; 2010; 144-145
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243858
identifier_str_mv Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice; Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010; Córdoba; Argentina; 2010; 144-145
CONICET Digital
CONICET
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