Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice
- Autores
- Bucciarelli, Alejandro; Bras, Cristina Liliana; Gandini, Norberto Ariel; Guardiola, Florencia Magalí; Minetti, Alejandra; Skliar, Mario Ignacio
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety.
Fil: Bucciarelli, Alejandro. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bras, Cristina Liliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Guardiola, Florencia Magalí. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Minetti, Alejandra. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Skliar, Mario Ignacio. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010
Córdoba
Argentina
Universidad Nacional de Cordoba
Universidad Nacional de Rosario - Materia
-
TOXICITY
SOLIDAGO CHILENSIS
SUBACUTE
EVALUATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/243858
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Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in miceBucciarelli, AlejandroBras, Cristina LilianaGandini, Norberto ArielGuardiola, Florencia MagalíMinetti, AlejandraSkliar, Mario IgnacioTOXICITYSOLIDAGO CHILENSISSUBACUTEEVALUATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety.Fil: Bucciarelli, Alejandro. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bras, Cristina Liliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Guardiola, Florencia Magalí. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Minetti, Alejandra. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Skliar, Mario Ignacio. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaPrimera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010CórdobaArgentinaUniversidad Nacional de CordobaUniversidad Nacional de RosarioUniversidad Nacional de Cordoba; Universidad Nacional de Rosario2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243858Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice; Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010; Córdoba; Argentina; 2010; 144-145CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ricifa.com.ar/wp-content/uploads/2021/09/Resumenes-2010.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:43Zoai:ri.conicet.gov.ar:11336/243858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:43.633CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
title |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
spellingShingle |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice Bucciarelli, Alejandro TOXICITY SOLIDAGO CHILENSIS SUBACUTE EVALUATION |
title_short |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
title_full |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
title_fullStr |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
title_full_unstemmed |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
title_sort |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice |
dc.creator.none.fl_str_mv |
Bucciarelli, Alejandro Bras, Cristina Liliana Gandini, Norberto Ariel Guardiola, Florencia Magalí Minetti, Alejandra Skliar, Mario Ignacio |
author |
Bucciarelli, Alejandro |
author_facet |
Bucciarelli, Alejandro Bras, Cristina Liliana Gandini, Norberto Ariel Guardiola, Florencia Magalí Minetti, Alejandra Skliar, Mario Ignacio |
author_role |
author |
author2 |
Bras, Cristina Liliana Gandini, Norberto Ariel Guardiola, Florencia Magalí Minetti, Alejandra Skliar, Mario Ignacio |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
TOXICITY SOLIDAGO CHILENSIS SUBACUTE EVALUATION |
topic |
TOXICITY SOLIDAGO CHILENSIS SUBACUTE EVALUATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety. Fil: Bucciarelli, Alejandro. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bras, Cristina Liliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Guardiola, Florencia Magalí. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Minetti, Alejandra. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Skliar, Mario Ignacio. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010 Córdoba Argentina Universidad Nacional de Cordoba Universidad Nacional de Rosario |
description |
Solidago species have been used in folk medicine for the treatment of several diseases. In South America, the most abundant species is Solidago chilensis Meyen (Asteraceae), widely used in traditional medicine of several countries, where is usually employed as a diuretic, analgesic, anti-inflammatory, and to treat gastrointestinal disorders (1). We have recently reported that aqueous extracts from inflorescences of S. chilensis protected gastric mucosa in mice subjected to an ethanol-induced gastric ulcer model (2). However, there are no toxicological studies available which would be necessary in order to confirm the safe usage of the plant. For this reason, the aim of the present work was to study the subacute oral toxicity of the aqueous extract from inflorescences of S. chilensis after a 28-day repeated exposure in mice by means of a Functional Observational Battery (FOB) and by assessing the motor activity in an open field. Materials and Methods The experiment was conduced according to the protocols described by OECD (3). A group of healthy CF1 mice (8 males and 8 females) were daily exposed to 1000 mg/kg of body weight of the extract mixed with the standard food for 28 days. A control group was fed only with the standard diet. All the animals were weekly observed for signs of toxicity. At the end of the exposure, behavioral and functional parameters were evaluated through a FOB, which included a thorough description of the animals’ appearance, behavior and functional integrity. This was assessed through observations in the home cage, while animals were moving freely in an open field, and through manipulative tests. The motor activity was assessed in an open field whose floor was divided into squares. The number of squares entered by mice with all four paws, rearings, groomings and fecal boluses were scored for 15 min. Results The subacute exposure to S. chilensis did not produce alterations in all parameters evaluated in the FOB or during the manipulative tests. No significant differences were observed between control and experimental groups in the different parameters analyzed during home cage, hand-held and open field observations (P>0.05). Motor activity evaluations in the square open field indicated that the subacute exposure did not modify neither the number of squares crossed nor rearings after the exposure. No significant differences were observed between control and experimental groups in emotionality parameters as the number of groomings and fecal boluses (P>0.05). Conclusions This study demonstrates that the aqueous extract from Solidago chilensis does not produce neurotoxicity due to it does not affect the functionality of the nervous system at neuromuscular, sensory and autonomic level. Considering the results it was demonstrated that the oral administration of the aqueous extract of the plant does not produce toxicity by subacute exposure in mice. This effect has already been observed in previous studies after the acute exposure in mice (4). Despite the lack of toxicity indicates that the possible therapeutic use of the plant may be safe, future research like potential chronic toxicity associated with this extract will need to be evaluated through long-term bioassays in order to ensure its safety. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 |
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info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
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conferenceObject |
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http://hdl.handle.net/11336/243858 Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice; Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010; Córdoba; Argentina; 2010; 144-145 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/243858 |
identifier_str_mv |
Evaluation of subacute toxicity of aqueous extarct from capitula of Solidago chilensis in mice; Primera Reunión Internacional de Ciencias Farmacéuticas - RICIFa 2010; Córdoba; Argentina; 2010; 144-145 CONICET Digital CONICET |
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eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/url/http://ricifa.com.ar/wp-content/uploads/2021/09/Resumenes-2010.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
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Nacional |
dc.publisher.none.fl_str_mv |
Universidad Nacional de Cordoba; Universidad Nacional de Rosario |
publisher.none.fl_str_mv |
Universidad Nacional de Cordoba; Universidad Nacional de Rosario |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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