Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust

Autores
Mendieta, Carolina Monica; Felissia, Fernando Esteban; Arismendy Pabón, Ana María; Kruyeniski, Julia; Area, Maria Cristina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).
Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Felissia, Fernando Esteban. Universidad Nacional de Misiones; Argentina
Fil: Arismendy Pabón, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Materia
BIOREFINERY
PINE SAWDUST
BIOETHANOL
KINETIC MODEL
MICHAELIS-MENTEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151070
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151070
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdustMendieta, Carolina MonicaFelissia, Fernando EstebanArismendy Pabón, Ana MaríaKruyeniski, JuliaArea, Maria CristinaBIOREFINERYPINE SAWDUSTBIOETHANOLKINETIC MODELMICHAELIS-MENTENhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Felissia, Fernando Esteban. Universidad Nacional de Misiones; ArgentinaFil: Arismendy Pabón, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaNorth Carolina State University. Department of Wood and Paper Science2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151070Mendieta, Carolina Monica; Felissia, Fernando Esteban; Arismendy Pabón, Ana María; Kruyeniski, Julia; Area, Maria Cristina; Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust; North Carolina State University. Department of Wood and Paper Science; Bioresources; 16; 4; 9-2021; 7474-74911930-2126CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_16_4_7474_Mendieta_Enzymatic_Hydrolysis_Fermentation_Strategiesinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:39Zoai:ri.conicet.gov.ar:11336/151070instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:39.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
title Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
spellingShingle Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
Mendieta, Carolina Monica
BIOREFINERY
PINE SAWDUST
BIOETHANOL
KINETIC MODEL
MICHAELIS-MENTEN
title_short Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
title_full Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
title_fullStr Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
title_full_unstemmed Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
title_sort Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
dc.creator.none.fl_str_mv Mendieta, Carolina Monica
Felissia, Fernando Esteban
Arismendy Pabón, Ana María
Kruyeniski, Julia
Area, Maria Cristina
author Mendieta, Carolina Monica
author_facet Mendieta, Carolina Monica
Felissia, Fernando Esteban
Arismendy Pabón, Ana María
Kruyeniski, Julia
Area, Maria Cristina
author_role author
author2 Felissia, Fernando Esteban
Arismendy Pabón, Ana María
Kruyeniski, Julia
Area, Maria Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOREFINERY
PINE SAWDUST
BIOETHANOL
KINETIC MODEL
MICHAELIS-MENTEN
topic BIOREFINERY
PINE SAWDUST
BIOETHANOL
KINETIC MODEL
MICHAELIS-MENTEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).
Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Felissia, Fernando Esteban. Universidad Nacional de Misiones; Argentina
Fil: Arismendy Pabón, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
description This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151070
Mendieta, Carolina Monica; Felissia, Fernando Esteban; Arismendy Pabón, Ana María; Kruyeniski, Julia; Area, Maria Cristina; Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust; North Carolina State University. Department of Wood and Paper Science; Bioresources; 16; 4; 9-2021; 7474-7491
1930-2126
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151070
identifier_str_mv Mendieta, Carolina Monica; Felissia, Fernando Esteban; Arismendy Pabón, Ana María; Kruyeniski, Julia; Area, Maria Cristina; Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust; North Carolina State University. Department of Wood and Paper Science; Bioresources; 16; 4; 9-2021; 7474-7491
1930-2126
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_16_4_7474_Mendieta_Enzymatic_Hydrolysis_Fermentation_Strategies
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv North Carolina State University. Department of Wood and Paper Science
publisher.none.fl_str_mv North Carolina State University. Department of Wood and Paper Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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