Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery

Autores
Mendieta, Carolina Monica; Kruyeniski, Julia; Felissia, Fernando Esteban; Area, Maria Cristina
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The decline in world oil reserves evidences the need to diversify the sources of equivalent raw materials. The use of biomass is one of the most explored alternatives. This work evaluates the second-generation bioethanol (2G) production from a pine sawdust soda-ethanol pulp by simultaneous hydrolysis and fermentation (SSF) to obtain the best conditions for scaling up the process. Experimental designs have been used to find mathematical models that define the complex situation jointly varying time with other variables (enzyme load and temperature). Time periods in the full model varied from 0 h to 72 h. Given the results (curve shape differences), it was decided to split the design in two, covering periods from 0 h to 24 h (Model I) and from 24 to 72 h (Model II). The pulp chemical composition was 80.2% glucans, 7.2% xylans, 0.3% galactans, 8.4% mannans, 3.7% lignin. Cellic® Ctec2 cellulolytic enzymes were used for saccharification and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast for fermentation. The best conditions found in the two designs were 30 FPU g−1 glucans, 39°C, 24 h for Model I, and 30 FPU g−1 glucans, 35°C, 72 h for Model II. Fermentation optimal values were 63.23 and 81.93 for Models I and II, respectively.
Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Felissia, Fernando Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Materia
MULTILEVEL FACTORIAL DESIGN
PINE SAWDUST
SODA-ETHANOL PRETREATMENT
SSF
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163646
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163646
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust BiorefineryMendieta, Carolina MonicaKruyeniski, JuliaFelissia, Fernando EstebanArea, Maria CristinaMULTILEVEL FACTORIAL DESIGNPINE SAWDUSTSODA-ETHANOL PRETREATMENTSSFhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2The decline in world oil reserves evidences the need to diversify the sources of equivalent raw materials. The use of biomass is one of the most explored alternatives. This work evaluates the second-generation bioethanol (2G) production from a pine sawdust soda-ethanol pulp by simultaneous hydrolysis and fermentation (SSF) to obtain the best conditions for scaling up the process. Experimental designs have been used to find mathematical models that define the complex situation jointly varying time with other variables (enzyme load and temperature). Time periods in the full model varied from 0 h to 72 h. Given the results (curve shape differences), it was decided to split the design in two, covering periods from 0 h to 24 h (Model I) and from 24 to 72 h (Model II). The pulp chemical composition was 80.2% glucans, 7.2% xylans, 0.3% galactans, 8.4% mannans, 3.7% lignin. Cellic® Ctec2 cellulolytic enzymes were used for saccharification and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast for fermentation. The best conditions found in the two designs were 30 FPU g−1 glucans, 39°C, 24 h for Model I, and 30 FPU g−1 glucans, 35°C, 72 h for Model II. Fermentation optimal values were 63.23 and 81.93 for Models I and II, respectively.Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Felissia, Fernando Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaMDPI AG2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163646Mendieta, Carolina Monica; Kruyeniski, Julia; Felissia, Fernando Esteban; Area, Maria Cristina; Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery; MDPI AG; Fermentation; 8; 3; 3-2022; 1-112311-5637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/8/3/130info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation8030130info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:45Zoai:ri.conicet.gov.ar:11336/163646instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:45.761CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
title Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
spellingShingle Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
Mendieta, Carolina Monica
MULTILEVEL FACTORIAL DESIGN
PINE SAWDUST
SODA-ETHANOL PRETREATMENT
SSF
title_short Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
title_full Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
title_fullStr Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
title_full_unstemmed Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
title_sort Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery
dc.creator.none.fl_str_mv Mendieta, Carolina Monica
Kruyeniski, Julia
Felissia, Fernando Esteban
Area, Maria Cristina
author Mendieta, Carolina Monica
author_facet Mendieta, Carolina Monica
Kruyeniski, Julia
Felissia, Fernando Esteban
Area, Maria Cristina
author_role author
author2 Kruyeniski, Julia
Felissia, Fernando Esteban
Area, Maria Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv MULTILEVEL FACTORIAL DESIGN
PINE SAWDUST
SODA-ETHANOL PRETREATMENT
SSF
topic MULTILEVEL FACTORIAL DESIGN
PINE SAWDUST
SODA-ETHANOL PRETREATMENT
SSF
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The decline in world oil reserves evidences the need to diversify the sources of equivalent raw materials. The use of biomass is one of the most explored alternatives. This work evaluates the second-generation bioethanol (2G) production from a pine sawdust soda-ethanol pulp by simultaneous hydrolysis and fermentation (SSF) to obtain the best conditions for scaling up the process. Experimental designs have been used to find mathematical models that define the complex situation jointly varying time with other variables (enzyme load and temperature). Time periods in the full model varied from 0 h to 72 h. Given the results (curve shape differences), it was decided to split the design in two, covering periods from 0 h to 24 h (Model I) and from 24 to 72 h (Model II). The pulp chemical composition was 80.2% glucans, 7.2% xylans, 0.3% galactans, 8.4% mannans, 3.7% lignin. Cellic® Ctec2 cellulolytic enzymes were used for saccharification and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast for fermentation. The best conditions found in the two designs were 30 FPU g−1 glucans, 39°C, 24 h for Model I, and 30 FPU g−1 glucans, 35°C, 72 h for Model II. Fermentation optimal values were 63.23 and 81.93 for Models I and II, respectively.
Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Felissia, Fernando Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
Fil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
description The decline in world oil reserves evidences the need to diversify the sources of equivalent raw materials. The use of biomass is one of the most explored alternatives. This work evaluates the second-generation bioethanol (2G) production from a pine sawdust soda-ethanol pulp by simultaneous hydrolysis and fermentation (SSF) to obtain the best conditions for scaling up the process. Experimental designs have been used to find mathematical models that define the complex situation jointly varying time with other variables (enzyme load and temperature). Time periods in the full model varied from 0 h to 72 h. Given the results (curve shape differences), it was decided to split the design in two, covering periods from 0 h to 24 h (Model I) and from 24 to 72 h (Model II). The pulp chemical composition was 80.2% glucans, 7.2% xylans, 0.3% galactans, 8.4% mannans, 3.7% lignin. Cellic® Ctec2 cellulolytic enzymes were used for saccharification and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast for fermentation. The best conditions found in the two designs were 30 FPU g−1 glucans, 39°C, 24 h for Model I, and 30 FPU g−1 glucans, 35°C, 72 h for Model II. Fermentation optimal values were 63.23 and 81.93 for Models I and II, respectively.
publishDate 2022
dc.date.none.fl_str_mv 2022-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163646
Mendieta, Carolina Monica; Kruyeniski, Julia; Felissia, Fernando Esteban; Area, Maria Cristina; Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery; MDPI AG; Fermentation; 8; 3; 3-2022; 1-11
2311-5637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163646
identifier_str_mv Mendieta, Carolina Monica; Kruyeniski, Julia; Felissia, Fernando Esteban; Area, Maria Cristina; Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery; MDPI AG; Fermentation; 8; 3; 3-2022; 1-11
2311-5637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/8/3/130
info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation8030130
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269715642187776
score 13.13397

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