Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats

Autores
Mancini, Jorge Eduardo; Croxatto, Juan Oscar; Gallo, Juan Eduardo Maria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: To examine the presence of diabetic retinopathy in a female rat model of type 2 diabetes fed on a high-fat diet (HFD). Methods: Wistar rats were injected with streptozotocin (STZ) at the age of two days and fed on an HFD from eight weeks onwards. Five diabetic animals were euthanized at 110 weeks of disease, together with a control group of age-matched, non-diabetic animals. A group of diabetic animals at 57 weeks of disease was included for comparison. Cross sections of the rats’ corneas, iris and retinas were histologically examined and analysed by immunohistochemistry and immunofluorescence, using glial-fibrillary-acidic-protein (GFAP), the vascular endothelial growth factor (VEGF) and the Von Willebrand factor (vWF). The trypsine digestive technique was used for the pericytes count. Results: Neovascularization was only found in the retinas, irises and corneas of the diabetic animals of 110 weeks of disease. There was also a significantly lower number of pericytes in these animals than in the controls. Conclusion: The female rat model of type 2 diabetes fed on an HFD may prove useful in evaluating the mechanisms involved in diabetic retinopathy, together with strategies to reduce its severity.
Fil: Mancini, Jorge Eduardo. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Croxatto, Juan Oscar. Fundación Oftalmología Argentina "J. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gallo, Juan Eduardo Maria. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Type 2 Diabetes
Diabetic Retinopathy
Diet
Neovascularization
Animal Model
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28422
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic ratsMancini, Jorge EduardoCroxatto, Juan OscarGallo, Juan Eduardo MariaType 2 DiabetesDiabetic RetinopathyDietNeovascularizationAnimal Modelhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: To examine the presence of diabetic retinopathy in a female rat model of type 2 diabetes fed on a high-fat diet (HFD). Methods: Wistar rats were injected with streptozotocin (STZ) at the age of two days and fed on an HFD from eight weeks onwards. Five diabetic animals were euthanized at 110 weeks of disease, together with a control group of age-matched, non-diabetic animals. A group of diabetic animals at 57 weeks of disease was included for comparison. Cross sections of the rats’ corneas, iris and retinas were histologically examined and analysed by immunohistochemistry and immunofluorescence, using glial-fibrillary-acidic-protein (GFAP), the vascular endothelial growth factor (VEGF) and the Von Willebrand factor (vWF). The trypsine digestive technique was used for the pericytes count. Results: Neovascularization was only found in the retinas, irises and corneas of the diabetic animals of 110 weeks of disease. There was also a significantly lower number of pericytes in these animals than in the controls. Conclusion: The female rat model of type 2 diabetes fed on an HFD may prove useful in evaluating the mechanisms involved in diabetic retinopathy, together with strategies to reduce its severity.Fil: Mancini, Jorge Eduardo. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Croxatto, Juan Oscar. Fundación Oftalmología Argentina "J. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gallo, Juan Eduardo Maria. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioMed Central2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28422Mancini, Jorge Eduardo; Croxatto, Juan Oscar; Gallo, Juan Eduardo Maria; Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats; BioMed Central; Diabetology & metabolic syndrome; 5; 1; 11-2013; 68-761758-5996CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dmsjournal.biomedcentral.com/articles/10.1186/1758-5996-5-68info:eu-repo/semantics/altIdentifier/doi/10.1186/1758-5996-5-68info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:19Zoai:ri.conicet.gov.ar:11336/28422instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:19.482CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
title Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
spellingShingle Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
Mancini, Jorge Eduardo
Type 2 Diabetes
Diabetic Retinopathy
Diet
Neovascularization
Animal Model
title_short Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
title_full Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
title_fullStr Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
title_full_unstemmed Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
title_sort Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats
dc.creator.none.fl_str_mv Mancini, Jorge Eduardo
Croxatto, Juan Oscar
Gallo, Juan Eduardo Maria
author Mancini, Jorge Eduardo
author_facet Mancini, Jorge Eduardo
Croxatto, Juan Oscar
Gallo, Juan Eduardo Maria
author_role author
author2 Croxatto, Juan Oscar
Gallo, Juan Eduardo Maria
author2_role author
author
dc.subject.none.fl_str_mv Type 2 Diabetes
Diabetic Retinopathy
Diet
Neovascularization
Animal Model
topic Type 2 Diabetes
Diabetic Retinopathy
Diet
Neovascularization
Animal Model
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: To examine the presence of diabetic retinopathy in a female rat model of type 2 diabetes fed on a high-fat diet (HFD). Methods: Wistar rats were injected with streptozotocin (STZ) at the age of two days and fed on an HFD from eight weeks onwards. Five diabetic animals were euthanized at 110 weeks of disease, together with a control group of age-matched, non-diabetic animals. A group of diabetic animals at 57 weeks of disease was included for comparison. Cross sections of the rats’ corneas, iris and retinas were histologically examined and analysed by immunohistochemistry and immunofluorescence, using glial-fibrillary-acidic-protein (GFAP), the vascular endothelial growth factor (VEGF) and the Von Willebrand factor (vWF). The trypsine digestive technique was used for the pericytes count. Results: Neovascularization was only found in the retinas, irises and corneas of the diabetic animals of 110 weeks of disease. There was also a significantly lower number of pericytes in these animals than in the controls. Conclusion: The female rat model of type 2 diabetes fed on an HFD may prove useful in evaluating the mechanisms involved in diabetic retinopathy, together with strategies to reduce its severity.
Fil: Mancini, Jorge Eduardo. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Croxatto, Juan Oscar. Fundación Oftalmología Argentina "J. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gallo, Juan Eduardo Maria. Universidad Austral. Facultad de Ciencias Biomédicas. Laboratorio de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: To examine the presence of diabetic retinopathy in a female rat model of type 2 diabetes fed on a high-fat diet (HFD). Methods: Wistar rats were injected with streptozotocin (STZ) at the age of two days and fed on an HFD from eight weeks onwards. Five diabetic animals were euthanized at 110 weeks of disease, together with a control group of age-matched, non-diabetic animals. A group of diabetic animals at 57 weeks of disease was included for comparison. Cross sections of the rats’ corneas, iris and retinas were histologically examined and analysed by immunohistochemistry and immunofluorescence, using glial-fibrillary-acidic-protein (GFAP), the vascular endothelial growth factor (VEGF) and the Von Willebrand factor (vWF). The trypsine digestive technique was used for the pericytes count. Results: Neovascularization was only found in the retinas, irises and corneas of the diabetic animals of 110 weeks of disease. There was also a significantly lower number of pericytes in these animals than in the controls. Conclusion: The female rat model of type 2 diabetes fed on an HFD may prove useful in evaluating the mechanisms involved in diabetic retinopathy, together with strategies to reduce its severity.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28422
Mancini, Jorge Eduardo; Croxatto, Juan Oscar; Gallo, Juan Eduardo Maria; Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats; BioMed Central; Diabetology & metabolic syndrome; 5; 1; 11-2013; 68-76
1758-5996
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28422
identifier_str_mv Mancini, Jorge Eduardo; Croxatto, Juan Oscar; Gallo, Juan Eduardo Maria; Proliferative retinopathy and neovascularization of the anterior segment in female type 2 diabetic rats; BioMed Central; Diabetology & metabolic syndrome; 5; 1; 11-2013; 68-76
1758-5996
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dmsjournal.biomedcentral.com/articles/10.1186/1758-5996-5-68
info:eu-repo/semantics/altIdentifier/doi/10.1186/1758-5996-5-68
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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