Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate

Autores
Lipponen, Katriina; Stege, Patricia Wanda; Cilpa, Geraldine; Samuelsson, Jörgen; Fornstedt, Torgny; Riekkola, Marja Liisa
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two different experimental approaches were used for obtaining a comprehensive view and understanding of the interactions between apolipoprotein B-100 (ApoB-100) of low-density lipoprotein and apolipoprotein E (ApoE) of high-density lipoprotein and chondroitin-6-sulfate (C6S) of arterial proteoglycan. The techniques employed were partial filling affinity capillary electrophoresis (PF-ACE) and continuous flow quartz crystal microbalance (QCM). In addition, molecular dynamic (MD) simulations were used to provide a supportive visual insight into the interaction mechanism. A new tool for analysis of QCM-data was utilized, i.e., adsorption energy distribution calculations, which allowed a deeper understanding of the interactions, especially at different temperatures. The PF-ACE technique probed mainly the strong adsorption interactions whereas in the MD calculations short-and long-range interactions could be distinguished. Although there are differences in the techniques, a pretty good agreement was achieved between the three approaches for the interaction of 19 amino acid peptide of ApoB with C6S giving log affinity constants of 4.66 by QCM, 5.02 by PF-ACE, and 7.39 by MD, and for 15 amino acid peptide of ApoE with C6S 5.34 by QCM, 5.28 by PT-ACE, and 4.60 by MD at physiological temperature 37.0 °C.
Fil: Lipponen, Katriina. University of Helsinki; Finlandia
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Cilpa, Geraldine. University of Helsinki; Finlandia
Fil: Samuelsson, Jörgen. Karlstad University; Suecia
Fil: Fornstedt, Torgny. Karlstad University; Suecia. Uppsala University; Suecia
Fil: Riekkola, Marja-Liisa. University of Helsinki; Finlandia
Materia
Lipoprotein Interactions
Chondroitin-6-Sulfate
Quartz Crystal Microbalance
Adsorption Energy Distribution
Partial Filling Capillary Affinity Electrophoresis
Molecular Dynamic Simulations
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93014
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93014
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfateLipponen, KatriinaStege, Patricia WandaCilpa, GeraldineSamuelsson, JörgenFornstedt, TorgnyRiekkola, Marja LiisaLipoprotein InteractionsChondroitin-6-SulfateQuartz Crystal MicrobalanceAdsorption Energy DistributionPartial Filling Capillary Affinity ElectrophoresisMolecular Dynamic Simulationshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Two different experimental approaches were used for obtaining a comprehensive view and understanding of the interactions between apolipoprotein B-100 (ApoB-100) of low-density lipoprotein and apolipoprotein E (ApoE) of high-density lipoprotein and chondroitin-6-sulfate (C6S) of arterial proteoglycan. The techniques employed were partial filling affinity capillary electrophoresis (PF-ACE) and continuous flow quartz crystal microbalance (QCM). In addition, molecular dynamic (MD) simulations were used to provide a supportive visual insight into the interaction mechanism. A new tool for analysis of QCM-data was utilized, i.e., adsorption energy distribution calculations, which allowed a deeper understanding of the interactions, especially at different temperatures. The PF-ACE technique probed mainly the strong adsorption interactions whereas in the MD calculations short-and long-range interactions could be distinguished. Although there are differences in the techniques, a pretty good agreement was achieved between the three approaches for the interaction of 19 amino acid peptide of ApoB with C6S giving log affinity constants of 4.66 by QCM, 5.02 by PF-ACE, and 7.39 by MD, and for 15 amino acid peptide of ApoE with C6S 5.34 by QCM, 5.28 by PT-ACE, and 4.60 by MD at physiological temperature 37.0 °C.Fil: Lipponen, Katriina. University of Helsinki; FinlandiaFil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Cilpa, Geraldine. University of Helsinki; FinlandiaFil: Samuelsson, Jörgen. Karlstad University; SueciaFil: Fornstedt, Torgny. Karlstad University; Suecia. Uppsala University; SueciaFil: Riekkola, Marja-Liisa. University of Helsinki; FinlandiaAmerican Chemical Society2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/93014Lipponen, Katriina; Stege, Patricia Wanda; Cilpa, Geraldine; Samuelsson, Jörgen; Fornstedt, Torgny; et al.; Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate; American Chemical Society; Analytical Chemistry; 83; 15; 8-2011; 6040-60460003-27001520-6882CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac201110cinfo:eu-repo/semantics/altIdentifier/doi/10.1021/ac201110cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:24Zoai:ri.conicet.gov.ar:11336/93014instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:24.741CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
title Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
spellingShingle Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
Lipponen, Katriina
Lipoprotein Interactions
Chondroitin-6-Sulfate
Quartz Crystal Microbalance
Adsorption Energy Distribution
Partial Filling Capillary Affinity Electrophoresis
Molecular Dynamic Simulations
title_short Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
title_full Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
title_fullStr Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
title_full_unstemmed Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
title_sort Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate
dc.creator.none.fl_str_mv Lipponen, Katriina
Stege, Patricia Wanda
Cilpa, Geraldine
Samuelsson, Jörgen
Fornstedt, Torgny
Riekkola, Marja Liisa
author Lipponen, Katriina
author_facet Lipponen, Katriina
Stege, Patricia Wanda
Cilpa, Geraldine
Samuelsson, Jörgen
Fornstedt, Torgny
Riekkola, Marja Liisa
author_role author
author2 Stege, Patricia Wanda
Cilpa, Geraldine
Samuelsson, Jörgen
Fornstedt, Torgny
Riekkola, Marja Liisa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Lipoprotein Interactions
Chondroitin-6-Sulfate
Quartz Crystal Microbalance
Adsorption Energy Distribution
Partial Filling Capillary Affinity Electrophoresis
Molecular Dynamic Simulations
topic Lipoprotein Interactions
Chondroitin-6-Sulfate
Quartz Crystal Microbalance
Adsorption Energy Distribution
Partial Filling Capillary Affinity Electrophoresis
Molecular Dynamic Simulations
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two different experimental approaches were used for obtaining a comprehensive view and understanding of the interactions between apolipoprotein B-100 (ApoB-100) of low-density lipoprotein and apolipoprotein E (ApoE) of high-density lipoprotein and chondroitin-6-sulfate (C6S) of arterial proteoglycan. The techniques employed were partial filling affinity capillary electrophoresis (PF-ACE) and continuous flow quartz crystal microbalance (QCM). In addition, molecular dynamic (MD) simulations were used to provide a supportive visual insight into the interaction mechanism. A new tool for analysis of QCM-data was utilized, i.e., adsorption energy distribution calculations, which allowed a deeper understanding of the interactions, especially at different temperatures. The PF-ACE technique probed mainly the strong adsorption interactions whereas in the MD calculations short-and long-range interactions could be distinguished. Although there are differences in the techniques, a pretty good agreement was achieved between the three approaches for the interaction of 19 amino acid peptide of ApoB with C6S giving log affinity constants of 4.66 by QCM, 5.02 by PF-ACE, and 7.39 by MD, and for 15 amino acid peptide of ApoE with C6S 5.34 by QCM, 5.28 by PT-ACE, and 4.60 by MD at physiological temperature 37.0 °C.
Fil: Lipponen, Katriina. University of Helsinki; Finlandia
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Cilpa, Geraldine. University of Helsinki; Finlandia
Fil: Samuelsson, Jörgen. Karlstad University; Suecia
Fil: Fornstedt, Torgny. Karlstad University; Suecia. Uppsala University; Suecia
Fil: Riekkola, Marja-Liisa. University of Helsinki; Finlandia
description Two different experimental approaches were used for obtaining a comprehensive view and understanding of the interactions between apolipoprotein B-100 (ApoB-100) of low-density lipoprotein and apolipoprotein E (ApoE) of high-density lipoprotein and chondroitin-6-sulfate (C6S) of arterial proteoglycan. The techniques employed were partial filling affinity capillary electrophoresis (PF-ACE) and continuous flow quartz crystal microbalance (QCM). In addition, molecular dynamic (MD) simulations were used to provide a supportive visual insight into the interaction mechanism. A new tool for analysis of QCM-data was utilized, i.e., adsorption energy distribution calculations, which allowed a deeper understanding of the interactions, especially at different temperatures. The PF-ACE technique probed mainly the strong adsorption interactions whereas in the MD calculations short-and long-range interactions could be distinguished. Although there are differences in the techniques, a pretty good agreement was achieved between the three approaches for the interaction of 19 amino acid peptide of ApoB with C6S giving log affinity constants of 4.66 by QCM, 5.02 by PF-ACE, and 7.39 by MD, and for 15 amino acid peptide of ApoE with C6S 5.34 by QCM, 5.28 by PT-ACE, and 4.60 by MD at physiological temperature 37.0 °C.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93014
Lipponen, Katriina; Stege, Patricia Wanda; Cilpa, Geraldine; Samuelsson, Jörgen; Fornstedt, Torgny; et al.; Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate; American Chemical Society; Analytical Chemistry; 83; 15; 8-2011; 6040-6046
0003-2700
1520-6882
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93014
identifier_str_mv Lipponen, Katriina; Stege, Patricia Wanda; Cilpa, Geraldine; Samuelsson, Jörgen; Fornstedt, Torgny; et al.; Three Different Approaches for the Clarification of the Interactions between Lipoproteins and Chondroitin-6-sulfate; American Chemical Society; Analytical Chemistry; 83; 15; 8-2011; 6040-6046
0003-2700
1520-6882
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac201110c
info:eu-repo/semantics/altIdentifier/doi/10.1021/ac201110c
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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