Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains

Autores
Suárez Esquivel, Marcela; Ruiz Villalobos, Nazareth; Castillo Zeledón, Amanda; Jiménez Rojas, César; Roop II, R. Martin; Comerci, Diego José; Barquero Calvo, Elías; Chacón Díaz, Carlos; Caswell, Clayton C.; Baker, Kate S.; Chaves Olarte, Esteban; Thomson, Nicholas R.; Moreno, Edgardo; Letesson, Jean J.; De Bolle, Xavier; Guzmán Verri, Caterina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing analysis of the reference strain B. abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version through a link at https://en.wikipedia.org/wiki/Brucella#Genomics. Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.
Fil: Suárez Esquivel, Marcela. Universidad Nacional de Costa Rica; Costa Rica
Fil: Ruiz Villalobos, Nazareth. Universidad Nacional de Costa Rica; Costa Rica
Fil: Castillo Zeledón, Amanda. Universidad Nacional de Costa Rica; Costa Rica
Fil: Jiménez Rojas, César. Universidad Nacional de Costa Rica; Costa Rica
Fil: Roop II, R. Martin. East Carolina University; Estados Unidos
Fil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Barquero Calvo, Elías. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa Rica
Fil: Chacón Díaz, Carlos. Universidad de Costa Rica; Costa Rica. Universidad Nacional de Costa Rica; Costa Rica
Fil: Caswell, Clayton C.. Virginia Tech University; Estados Unidos
Fil: Baker, Kate S.. University of Liverpool; Reino Unido. Wellcome Trust Sanger Institute; Reino Unido
Fil: Chaves Olarte, Esteban. Universidad de Costa Rica; Costa Rica
Fil: Thomson, Nicholas R.. Wellcome Trust Sanger Institute; Reino Unido. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Moreno, Edgardo. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa Rica
Fil: Letesson, Jean J.. Université de Namur. Unité de Recherche en Biologie des Microorganismes; Bélgica
Fil: De Bolle, Xavier. Université de Namur. Unité de Recherche en Biologie des Microorganismes; Bélgica
Fil: Guzmán Verri, Caterina. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa Rica
Materia
BRUCELLA BRUCELLA ABORTUS
REFERENCE GENOME
REFERENCE STRAIN
WGS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54913
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54913
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strainsSuárez Esquivel, MarcelaRuiz Villalobos, NazarethCastillo Zeledón, AmandaJiménez Rojas, CésarRoop II, R. MartinComerci, Diego JoséBarquero Calvo, ElíasChacón Díaz, CarlosCaswell, Clayton C.Baker, Kate S.Chaves Olarte, EstebanThomson, Nicholas R.Moreno, EdgardoLetesson, Jean J.De Bolle, XavierGuzmán Verri, CaterinaBRUCELLA BRUCELLA ABORTUSREFERENCE GENOMEREFERENCE STRAINWGShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing analysis of the reference strain B. abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version through a link at https://en.wikipedia.org/wiki/Brucella#Genomics. Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.Fil: Suárez Esquivel, Marcela. Universidad Nacional de Costa Rica; Costa RicaFil: Ruiz Villalobos, Nazareth. Universidad Nacional de Costa Rica; Costa RicaFil: Castillo Zeledón, Amanda. Universidad Nacional de Costa Rica; Costa RicaFil: Jiménez Rojas, César. Universidad Nacional de Costa Rica; Costa RicaFil: Roop II, R. Martin. East Carolina University; Estados UnidosFil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Barquero Calvo, Elías. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa RicaFil: Chacón Díaz, Carlos. Universidad de Costa Rica; Costa Rica. Universidad Nacional de Costa Rica; Costa RicaFil: Caswell, Clayton C.. Virginia Tech University; Estados UnidosFil: Baker, Kate S.. University of Liverpool; Reino Unido. Wellcome Trust Sanger Institute; Reino UnidoFil: Chaves Olarte, Esteban. Universidad de Costa Rica; Costa RicaFil: Thomson, Nicholas R.. Wellcome Trust Sanger Institute; Reino Unido. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Moreno, Edgardo. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa RicaFil: Letesson, Jean J.. Université de Namur. Unité de Recherche en Biologie des Microorganismes; BélgicaFil: De Bolle, Xavier. Université de Namur. Unité de Recherche en Biologie des Microorganismes; BélgicaFil: Guzmán Verri, Caterina. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa RicaFrontiers Research Foundation2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54913Suárez Esquivel, Marcela; Ruiz Villalobos, Nazareth; Castillo Zeledón, Amanda; Jiménez Rojas, César; Roop II, R. Martin; et al.; Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains; Frontiers Research Foundation; Frontiers in Microbiology; 7; 9-2016; 1-71664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2016.01557info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2016.01557/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:45Zoai:ri.conicet.gov.ar:11336/54913instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:45.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
title Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
spellingShingle Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
Suárez Esquivel, Marcela
BRUCELLA BRUCELLA ABORTUS
REFERENCE GENOME
REFERENCE STRAIN
WGS
title_short Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
title_full Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
title_fullStr Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
title_full_unstemmed Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
title_sort Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains
dc.creator.none.fl_str_mv Suárez Esquivel, Marcela
Ruiz Villalobos, Nazareth
Castillo Zeledón, Amanda
Jiménez Rojas, César
Roop II, R. Martin
Comerci, Diego José
Barquero Calvo, Elías
Chacón Díaz, Carlos
Caswell, Clayton C.
Baker, Kate S.
Chaves Olarte, Esteban
Thomson, Nicholas R.
Moreno, Edgardo
Letesson, Jean J.
De Bolle, Xavier
Guzmán Verri, Caterina
author Suárez Esquivel, Marcela
author_facet Suárez Esquivel, Marcela
Ruiz Villalobos, Nazareth
Castillo Zeledón, Amanda
Jiménez Rojas, César
Roop II, R. Martin
Comerci, Diego José
Barquero Calvo, Elías
Chacón Díaz, Carlos
Caswell, Clayton C.
Baker, Kate S.
Chaves Olarte, Esteban
Thomson, Nicholas R.
Moreno, Edgardo
Letesson, Jean J.
De Bolle, Xavier
Guzmán Verri, Caterina
author_role author
author2 Ruiz Villalobos, Nazareth
Castillo Zeledón, Amanda
Jiménez Rojas, César
Roop II, R. Martin
Comerci, Diego José
Barquero Calvo, Elías
Chacón Díaz, Carlos
Caswell, Clayton C.
Baker, Kate S.
Chaves Olarte, Esteban
Thomson, Nicholas R.
Moreno, Edgardo
Letesson, Jean J.
De Bolle, Xavier
Guzmán Verri, Caterina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BRUCELLA BRUCELLA ABORTUS
REFERENCE GENOME
REFERENCE STRAIN
WGS
topic BRUCELLA BRUCELLA ABORTUS
REFERENCE GENOME
REFERENCE STRAIN
WGS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing analysis of the reference strain B. abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version through a link at https://en.wikipedia.org/wiki/Brucella#Genomics. Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.
Fil: Suárez Esquivel, Marcela. Universidad Nacional de Costa Rica; Costa Rica
Fil: Ruiz Villalobos, Nazareth. Universidad Nacional de Costa Rica; Costa Rica
Fil: Castillo Zeledón, Amanda. Universidad Nacional de Costa Rica; Costa Rica
Fil: Jiménez Rojas, César. Universidad Nacional de Costa Rica; Costa Rica
Fil: Roop II, R. Martin. East Carolina University; Estados Unidos
Fil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Barquero Calvo, Elías. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa Rica
Fil: Chacón Díaz, Carlos. Universidad de Costa Rica; Costa Rica. Universidad Nacional de Costa Rica; Costa Rica
Fil: Caswell, Clayton C.. Virginia Tech University; Estados Unidos
Fil: Baker, Kate S.. University of Liverpool; Reino Unido. Wellcome Trust Sanger Institute; Reino Unido
Fil: Chaves Olarte, Esteban. Universidad de Costa Rica; Costa Rica
Fil: Thomson, Nicholas R.. Wellcome Trust Sanger Institute; Reino Unido. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Moreno, Edgardo. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa Rica
Fil: Letesson, Jean J.. Université de Namur. Unité de Recherche en Biologie des Microorganismes; Bélgica
Fil: De Bolle, Xavier. Université de Namur. Unité de Recherche en Biologie des Microorganismes; Bélgica
Fil: Guzmán Verri, Caterina. Universidad Nacional de Costa Rica; Costa Rica. Universidad de Costa Rica; Costa Rica
description Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing analysis of the reference strain B. abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version through a link at https://en.wikipedia.org/wiki/Brucella#Genomics. Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54913
Suárez Esquivel, Marcela; Ruiz Villalobos, Nazareth; Castillo Zeledón, Amanda; Jiménez Rojas, César; Roop II, R. Martin; et al.; Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains; Frontiers Research Foundation; Frontiers in Microbiology; 7; 9-2016; 1-7
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54913
identifier_str_mv Suárez Esquivel, Marcela; Ruiz Villalobos, Nazareth; Castillo Zeledón, Amanda; Jiménez Rojas, César; Roop II, R. Martin; et al.; Brucella abortus strain 2308 Wisconsin genome: Importance of the definition of reference strains; Frontiers Research Foundation; Frontiers in Microbiology; 7; 9-2016; 1-7
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2016.01557
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2016.01557/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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