A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication

Autores
del Giudice, Mariela Giselda; Ugalde, Juan Esteban; Czibener, Cecilia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Secretion of proteins in Gram-negative bacteria is a high-energy consuming process that requires the translocation across two membranes and a periplasmic space composed of a mesh like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers and, in many cases, there are specific peptidoglycanases that degrade the peptidoglycan to allow the proper assembly of the secretion machinery. We describe here the identification and characterization of a muramidase in Brucella abortus that participates in the intracellular multiplication in professional and non-professional phagocytes. We demonstrated that this protein has peptidoglycanase activity, that a strain with a clean deletion of the gene displayed a defect in the early stages of the intracellular multiplication curve and that this is dependent on the lytic activity. While neither the attachment nor the invasion of the strain was affected we demonstrated that it had a defect in excluding the lysosomal marker LAMP-1 but not in acquiring the reticulum endoplasmic marker calnexin, indicating that the gene participates in the early stages of the intracellular trafficking but not in the establishment of the replicative niche. Analysis of the assembly status and functionality of the VirB secretion apparatus indicated that the mutant has affected the proper function of this central virulence factor.
Fil: del Giudice, Mariela Giselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ugalde, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
BRUCELLA ABORTUS
LYSOZYME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24060
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replicationdel Giudice, Mariela GiseldaUgalde, Juan EstebanCzibener, CeciliaBRUCELLA ABORTUSLYSOZYMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Secretion of proteins in Gram-negative bacteria is a high-energy consuming process that requires the translocation across two membranes and a periplasmic space composed of a mesh like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers and, in many cases, there are specific peptidoglycanases that degrade the peptidoglycan to allow the proper assembly of the secretion machinery. We describe here the identification and characterization of a muramidase in Brucella abortus that participates in the intracellular multiplication in professional and non-professional phagocytes. We demonstrated that this protein has peptidoglycanase activity, that a strain with a clean deletion of the gene displayed a defect in the early stages of the intracellular multiplication curve and that this is dependent on the lytic activity. While neither the attachment nor the invasion of the strain was affected we demonstrated that it had a defect in excluding the lysosomal marker LAMP-1 but not in acquiring the reticulum endoplasmic marker calnexin, indicating that the gene participates in the early stages of the intracellular trafficking but not in the establishment of the replicative niche. Analysis of the assembly status and functionality of the VirB secretion apparatus indicated that the mutant has affected the proper function of this central virulence factor.Fil: del Giudice, Mariela Giselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Ugalde, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaAmerican Society for Microbiology2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24060del Giudice, Mariela Giselda; Ugalde, Juan Esteban; Czibener, Cecilia; A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication; American Society for Microbiology; Infection and Immunity; 81; 3; 3-2013; 956-9640019-9567CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.01158-12info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/81/3/956info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:00Zoai:ri.conicet.gov.ar:11336/24060instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:01.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
title A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
spellingShingle A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
del Giudice, Mariela Giselda
BRUCELLA ABORTUS
LYSOZYME
title_short A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
title_full A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
title_fullStr A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
title_full_unstemmed A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
title_sort A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication
dc.creator.none.fl_str_mv del Giudice, Mariela Giselda
Ugalde, Juan Esteban
Czibener, Cecilia
author del Giudice, Mariela Giselda
author_facet del Giudice, Mariela Giselda
Ugalde, Juan Esteban
Czibener, Cecilia
author_role author
author2 Ugalde, Juan Esteban
Czibener, Cecilia
author2_role author
author
dc.subject.none.fl_str_mv BRUCELLA ABORTUS
LYSOZYME
topic BRUCELLA ABORTUS
LYSOZYME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Secretion of proteins in Gram-negative bacteria is a high-energy consuming process that requires the translocation across two membranes and a periplasmic space composed of a mesh like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers and, in many cases, there are specific peptidoglycanases that degrade the peptidoglycan to allow the proper assembly of the secretion machinery. We describe here the identification and characterization of a muramidase in Brucella abortus that participates in the intracellular multiplication in professional and non-professional phagocytes. We demonstrated that this protein has peptidoglycanase activity, that a strain with a clean deletion of the gene displayed a defect in the early stages of the intracellular multiplication curve and that this is dependent on the lytic activity. While neither the attachment nor the invasion of the strain was affected we demonstrated that it had a defect in excluding the lysosomal marker LAMP-1 but not in acquiring the reticulum endoplasmic marker calnexin, indicating that the gene participates in the early stages of the intracellular trafficking but not in the establishment of the replicative niche. Analysis of the assembly status and functionality of the VirB secretion apparatus indicated that the mutant has affected the proper function of this central virulence factor.
Fil: del Giudice, Mariela Giselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ugalde, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
description Secretion of proteins in Gram-negative bacteria is a high-energy consuming process that requires the translocation across two membranes and a periplasmic space composed of a mesh like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers and, in many cases, there are specific peptidoglycanases that degrade the peptidoglycan to allow the proper assembly of the secretion machinery. We describe here the identification and characterization of a muramidase in Brucella abortus that participates in the intracellular multiplication in professional and non-professional phagocytes. We demonstrated that this protein has peptidoglycanase activity, that a strain with a clean deletion of the gene displayed a defect in the early stages of the intracellular multiplication curve and that this is dependent on the lytic activity. While neither the attachment nor the invasion of the strain was affected we demonstrated that it had a defect in excluding the lysosomal marker LAMP-1 but not in acquiring the reticulum endoplasmic marker calnexin, indicating that the gene participates in the early stages of the intracellular trafficking but not in the establishment of the replicative niche. Analysis of the assembly status and functionality of the VirB secretion apparatus indicated that the mutant has affected the proper function of this central virulence factor.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24060
del Giudice, Mariela Giselda; Ugalde, Juan Esteban; Czibener, Cecilia; A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication; American Society for Microbiology; Infection and Immunity; 81; 3; 3-2013; 956-964
0019-9567
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24060
identifier_str_mv del Giudice, Mariela Giselda; Ugalde, Juan Esteban; Czibener, Cecilia; A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication; American Society for Microbiology; Infection and Immunity; 81; 3; 3-2013; 956-964
0019-9567
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.01158-12
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/81/3/956
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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