Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols

Autores
Venegas, F. A.; Saona, L. A.; Monrás, J. P.; Órdenes Aenishanslins, N.; Giordana, María Florencia; Ulloa, G.; Collao, B.; Bravo, D.; Pérez Donoso, José M.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Developing methods with a low environmental impact for nanoparticle synthesis remains one of the greatest challenges in nanotechnology. In this context, biomimetic and biological methods have emerged as green chemistry alternatives, and also contribute to our understanding of how nanomaterials interact with cellular molecules. Here, we report a phosphate-dependent biomimetic method to synthesize of cadmium sulphide (CdS) QDs at low temperatures, physiological pH and aerobic conditions, using CdCl2 and thiols (l-cysteine, glutathione or mercaptosuccinic acid). Inorganic phosphate (Pi) and cellular phosphorylated intermediates such as adenosine monophosphate, glucose-6-phosphate, glycerol-2-phosphate and fructose-1,6-biphosphate, can trigger CdS QDs synthesis. The produced QDs are cubic phase nanocrystals with a tunable fluorescence (450-700 nm), small size (4-12 nm), and are composed of thiols and Pi. In CdS synthesis, the importance of the phosphate is related to its capacity to release H2S from thiols, a phenomenon associated with its base-properties. Based on the biomimetic method, we developed a Pi-based procedure to synthesize CdS QDs in Escherichia coli. As in the biomimetic procedure, Pi favors QDs-biosynthesis not only by mediating biological generation of H2S, but also by improving Cd2+ cellular uptake. A role for phosphates in the cellular interaction and green synthesis of metal QDs is discussed.
Fil: Venegas, F. A.. Universidad Andrés Bello; Chile
Fil: Saona, L. A.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile
Fil: Monrás, J. P.. Universidad Andrés Bello; Chile
Fil: Órdenes Aenishanslins, N.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile
Fil: Giordana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina
Fil: Ulloa, G.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile
Fil: Collao, B.. Universidad Andrés Bello; Chile
Fil: Bravo, D.. Universidad de Chile; Chile
Fil: Pérez Donoso, José M.. Universidad Andrés Bello; Chile
Materia
BIOMIMETIC SYNTHESIS
CADMIUM SULPHIDE
NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50494
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50494
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiolsVenegas, F. A.Saona, L. A.Monrás, J. P.Órdenes Aenishanslins, N.Giordana, María FlorenciaUlloa, G.Collao, B.Bravo, D.Pérez Donoso, José M.BIOMIMETIC SYNTHESISCADMIUM SULPHIDENANOPARTICLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Developing methods with a low environmental impact for nanoparticle synthesis remains one of the greatest challenges in nanotechnology. In this context, biomimetic and biological methods have emerged as green chemistry alternatives, and also contribute to our understanding of how nanomaterials interact with cellular molecules. Here, we report a phosphate-dependent biomimetic method to synthesize of cadmium sulphide (CdS) QDs at low temperatures, physiological pH and aerobic conditions, using CdCl2 and thiols (l-cysteine, glutathione or mercaptosuccinic acid). Inorganic phosphate (Pi) and cellular phosphorylated intermediates such as adenosine monophosphate, glucose-6-phosphate, glycerol-2-phosphate and fructose-1,6-biphosphate, can trigger CdS QDs synthesis. The produced QDs are cubic phase nanocrystals with a tunable fluorescence (450-700 nm), small size (4-12 nm), and are composed of thiols and Pi. In CdS synthesis, the importance of the phosphate is related to its capacity to release H2S from thiols, a phenomenon associated with its base-properties. Based on the biomimetic method, we developed a Pi-based procedure to synthesize CdS QDs in Escherichia coli. As in the biomimetic procedure, Pi favors QDs-biosynthesis not only by mediating biological generation of H2S, but also by improving Cd2+ cellular uptake. A role for phosphates in the cellular interaction and green synthesis of metal QDs is discussed.Fil: Venegas, F. A.. Universidad Andrés Bello; ChileFil: Saona, L. A.. Universidad Andrés Bello; Chile. Universidad de Chile; ChileFil: Monrás, J. P.. Universidad Andrés Bello; ChileFil: Órdenes Aenishanslins, N.. Universidad Andrés Bello; Chile. Universidad de Chile; ChileFil: Giordana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; ArgentinaFil: Ulloa, G.. Universidad Andrés Bello; Chile. Universidad de Chile; ChileFil: Collao, B.. Universidad Andrés Bello; ChileFil: Bravo, D.. Universidad de Chile; ChileFil: Pérez Donoso, José M.. Universidad Andrés Bello; ChileRoyal Society of Chemistry2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50494Venegas, F. A.; Saona, L. A.; Monrás, J. P.; Órdenes Aenishanslins, N.; Giordana, María Florencia; et al.; Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols; Royal Society of Chemistry; RSC Advances; 7; 64; 8-2017; 40270-402782046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C7RA03578Kinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C7RA03578Kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:23Zoai:ri.conicet.gov.ar:11336/50494instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:23.279CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
title Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
spellingShingle Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
Venegas, F. A.
BIOMIMETIC SYNTHESIS
CADMIUM SULPHIDE
NANOPARTICLES
title_short Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
title_full Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
title_fullStr Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
title_full_unstemmed Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
title_sort Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
dc.creator.none.fl_str_mv Venegas, F. A.
Saona, L. A.
Monrás, J. P.
Órdenes Aenishanslins, N.
Giordana, María Florencia
Ulloa, G.
Collao, B.
Bravo, D.
Pérez Donoso, José M.
author Venegas, F. A.
author_facet Venegas, F. A.
Saona, L. A.
Monrás, J. P.
Órdenes Aenishanslins, N.
Giordana, María Florencia
Ulloa, G.
Collao, B.
Bravo, D.
Pérez Donoso, José M.
author_role author
author2 Saona, L. A.
Monrás, J. P.
Órdenes Aenishanslins, N.
Giordana, María Florencia
Ulloa, G.
Collao, B.
Bravo, D.
Pérez Donoso, José M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOMIMETIC SYNTHESIS
CADMIUM SULPHIDE
NANOPARTICLES
topic BIOMIMETIC SYNTHESIS
CADMIUM SULPHIDE
NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Developing methods with a low environmental impact for nanoparticle synthesis remains one of the greatest challenges in nanotechnology. In this context, biomimetic and biological methods have emerged as green chemistry alternatives, and also contribute to our understanding of how nanomaterials interact with cellular molecules. Here, we report a phosphate-dependent biomimetic method to synthesize of cadmium sulphide (CdS) QDs at low temperatures, physiological pH and aerobic conditions, using CdCl2 and thiols (l-cysteine, glutathione or mercaptosuccinic acid). Inorganic phosphate (Pi) and cellular phosphorylated intermediates such as adenosine monophosphate, glucose-6-phosphate, glycerol-2-phosphate and fructose-1,6-biphosphate, can trigger CdS QDs synthesis. The produced QDs are cubic phase nanocrystals with a tunable fluorescence (450-700 nm), small size (4-12 nm), and are composed of thiols and Pi. In CdS synthesis, the importance of the phosphate is related to its capacity to release H2S from thiols, a phenomenon associated with its base-properties. Based on the biomimetic method, we developed a Pi-based procedure to synthesize CdS QDs in Escherichia coli. As in the biomimetic procedure, Pi favors QDs-biosynthesis not only by mediating biological generation of H2S, but also by improving Cd2+ cellular uptake. A role for phosphates in the cellular interaction and green synthesis of metal QDs is discussed.
Fil: Venegas, F. A.. Universidad Andrés Bello; Chile
Fil: Saona, L. A.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile
Fil: Monrás, J. P.. Universidad Andrés Bello; Chile
Fil: Órdenes Aenishanslins, N.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile
Fil: Giordana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina
Fil: Ulloa, G.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile
Fil: Collao, B.. Universidad Andrés Bello; Chile
Fil: Bravo, D.. Universidad de Chile; Chile
Fil: Pérez Donoso, José M.. Universidad Andrés Bello; Chile
description Developing methods with a low environmental impact for nanoparticle synthesis remains one of the greatest challenges in nanotechnology. In this context, biomimetic and biological methods have emerged as green chemistry alternatives, and also contribute to our understanding of how nanomaterials interact with cellular molecules. Here, we report a phosphate-dependent biomimetic method to synthesize of cadmium sulphide (CdS) QDs at low temperatures, physiological pH and aerobic conditions, using CdCl2 and thiols (l-cysteine, glutathione or mercaptosuccinic acid). Inorganic phosphate (Pi) and cellular phosphorylated intermediates such as adenosine monophosphate, glucose-6-phosphate, glycerol-2-phosphate and fructose-1,6-biphosphate, can trigger CdS QDs synthesis. The produced QDs are cubic phase nanocrystals with a tunable fluorescence (450-700 nm), small size (4-12 nm), and are composed of thiols and Pi. In CdS synthesis, the importance of the phosphate is related to its capacity to release H2S from thiols, a phenomenon associated with its base-properties. Based on the biomimetic method, we developed a Pi-based procedure to synthesize CdS QDs in Escherichia coli. As in the biomimetic procedure, Pi favors QDs-biosynthesis not only by mediating biological generation of H2S, but also by improving Cd2+ cellular uptake. A role for phosphates in the cellular interaction and green synthesis of metal QDs is discussed.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50494
Venegas, F. A.; Saona, L. A.; Monrás, J. P.; Órdenes Aenishanslins, N.; Giordana, María Florencia; et al.; Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols; Royal Society of Chemistry; RSC Advances; 7; 64; 8-2017; 40270-40278
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50494
identifier_str_mv Venegas, F. A.; Saona, L. A.; Monrás, J. P.; Órdenes Aenishanslins, N.; Giordana, María Florencia; et al.; Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols; Royal Society of Chemistry; RSC Advances; 7; 64; 8-2017; 40270-40278
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C7RA03578K
info:eu-repo/semantics/altIdentifier/doi/10.1039/C7RA03578K
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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