Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules

Autores
Jiang, Shuai; Prozeller, Domenik; Pereira, Jorge; Simon, Johanna; Han, Shen; Wirsching, Sebastian; Fichter, Michael; Mottola, Milagro; Lieberwirth, Ingo; Morsbach, Svenja; Mailänder, Volker; Gehring, Stephan; Crespy, Daniel; Landfester, Katharina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare core–shell silica nanocapsules (SiO2 NCs) via a sol–gel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mL−1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal murine liver cells significantly reduces the release of inflammatory cytokines, indicating an effective suppression of inflammatory response of liver macrophages. Fluorescent and magnetic labeling of the NCs allows for monitoring their intracellular trafficking and biodegradation. Controlled interaction with blood proteins and good colloidal stability in blood plasma are achieved via PEGylation of the NCs. Specific proteins responsible for stealth effect, such as apolipoprotein A-I, apolipoprotein A-IV, and clusterin, are present in large amounts on the PEGylated NCs. In vivo biodistribution investigations prove an efficient accumulation of NCs in the liver, underlining the suitability of the SiO2 NCs as a dexamethasone carrier for treating inflammatory liver diseases.
Fil: Jiang, Shuai. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Prozeller, Domenik. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Pereira, Jorge. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Simon, Johanna. Max-Planck-Institut für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Han, Shen. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Wirsching, Sebastian. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Fichter, Michael. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Mottola, Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Lieberwirth, Ingo. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Morsbach, Svenja. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Mailänder, Volker. Max-Planck-Institut für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Crespy, Daniel. Max-Planck-Institut für Polymerforschung; Alemania. Vidyasirimedhi Institute of Science and Technology; Tailandia
Fil: Landfester, Katharina. Max-Planck-Institut für Polymerforschung; Alemania
Materia
SILICA NANOCAPSULES
PROTEIN INTERACTIONS
DEXAMETHASONE
IMMUNOSUPPRESSIVE THERAPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111896
Acceder
id CONICETDig_ac19f185e2be0d676121ac370adc4f08
oai_identifier_str oai:ri.conicet.gov.ar:11336/111896
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsulesJiang, ShuaiProzeller, DomenikPereira, JorgeSimon, JohannaHan, ShenWirsching, SebastianFichter, MichaelMottola, MilagroLieberwirth, IngoMorsbach, SvenjaMailänder, VolkerGehring, StephanCrespy, DanielLandfester, KatharinaSILICA NANOCAPSULESPROTEIN INTERACTIONSDEXAMETHASONEIMMUNOSUPPRESSIVE THERAPYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare core–shell silica nanocapsules (SiO2 NCs) via a sol–gel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mL−1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal murine liver cells significantly reduces the release of inflammatory cytokines, indicating an effective suppression of inflammatory response of liver macrophages. Fluorescent and magnetic labeling of the NCs allows for monitoring their intracellular trafficking and biodegradation. Controlled interaction with blood proteins and good colloidal stability in blood plasma are achieved via PEGylation of the NCs. Specific proteins responsible for stealth effect, such as apolipoprotein A-I, apolipoprotein A-IV, and clusterin, are present in large amounts on the PEGylated NCs. In vivo biodistribution investigations prove an efficient accumulation of NCs in the liver, underlining the suitability of the SiO2 NCs as a dexamethasone carrier for treating inflammatory liver diseases.Fil: Jiang, Shuai. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Prozeller, Domenik. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Pereira, Jorge. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Simon, Johanna. Max-Planck-Institut für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Han, Shen. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Wirsching, Sebastian. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Fichter, Michael. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Mottola, Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Lieberwirth, Ingo. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Morsbach, Svenja. Max-Planck-Institut für Polymerforschung; AlemaniaFil: Mailänder, Volker. Max-Planck-Institut für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Crespy, Daniel. Max-Planck-Institut für Polymerforschung; Alemania. Vidyasirimedhi Institute of Science and Technology; TailandiaFil: Landfester, Katharina. Max-Planck-Institut für Polymerforschung; AlemaniaRoyal Society of Chemistry2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111896Jiang, Shuai; Prozeller, Domenik; Pereira, Jorge; Simon, Johanna; Han, Shen; et al.; Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules; Royal Society of Chemistry; Nanoscale; 12; 4; 1-2020; 2626-26372040-33642040-3372CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2020/NR/C9NR09879Hinfo:eu-repo/semantics/altIdentifier/doi/10.1039/c9nr09879hinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:33Zoai:ri.conicet.gov.ar:11336/111896instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:34.061CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
title Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
spellingShingle Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
Jiang, Shuai
SILICA NANOCAPSULES
PROTEIN INTERACTIONS
DEXAMETHASONE
IMMUNOSUPPRESSIVE THERAPY
title_short Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
title_full Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
title_fullStr Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
title_full_unstemmed Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
title_sort Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
dc.creator.none.fl_str_mv Jiang, Shuai
Prozeller, Domenik
Pereira, Jorge
Simon, Johanna
Han, Shen
Wirsching, Sebastian
Fichter, Michael
Mottola, Milagro
Lieberwirth, Ingo
Morsbach, Svenja
Mailänder, Volker
Gehring, Stephan
Crespy, Daniel
Landfester, Katharina
author Jiang, Shuai
author_facet Jiang, Shuai
Prozeller, Domenik
Pereira, Jorge
Simon, Johanna
Han, Shen
Wirsching, Sebastian
Fichter, Michael
Mottola, Milagro
Lieberwirth, Ingo
Morsbach, Svenja
Mailänder, Volker
Gehring, Stephan
Crespy, Daniel
Landfester, Katharina
author_role author
author2 Prozeller, Domenik
Pereira, Jorge
Simon, Johanna
Han, Shen
Wirsching, Sebastian
Fichter, Michael
Mottola, Milagro
Lieberwirth, Ingo
Morsbach, Svenja
Mailänder, Volker
Gehring, Stephan
Crespy, Daniel
Landfester, Katharina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SILICA NANOCAPSULES
PROTEIN INTERACTIONS
DEXAMETHASONE
IMMUNOSUPPRESSIVE THERAPY
topic SILICA NANOCAPSULES
PROTEIN INTERACTIONS
DEXAMETHASONE
IMMUNOSUPPRESSIVE THERAPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare core–shell silica nanocapsules (SiO2 NCs) via a sol–gel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mL−1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal murine liver cells significantly reduces the release of inflammatory cytokines, indicating an effective suppression of inflammatory response of liver macrophages. Fluorescent and magnetic labeling of the NCs allows for monitoring their intracellular trafficking and biodegradation. Controlled interaction with blood proteins and good colloidal stability in blood plasma are achieved via PEGylation of the NCs. Specific proteins responsible for stealth effect, such as apolipoprotein A-I, apolipoprotein A-IV, and clusterin, are present in large amounts on the PEGylated NCs. In vivo biodistribution investigations prove an efficient accumulation of NCs in the liver, underlining the suitability of the SiO2 NCs as a dexamethasone carrier for treating inflammatory liver diseases.
Fil: Jiang, Shuai. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Prozeller, Domenik. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Pereira, Jorge. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Simon, Johanna. Max-Planck-Institut für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Han, Shen. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Wirsching, Sebastian. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Fichter, Michael. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Mottola, Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Lieberwirth, Ingo. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Morsbach, Svenja. Max-Planck-Institut für Polymerforschung; Alemania
Fil: Mailänder, Volker. Max-Planck-Institut für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Crespy, Daniel. Max-Planck-Institut für Polymerforschung; Alemania. Vidyasirimedhi Institute of Science and Technology; Tailandia
Fil: Landfester, Katharina. Max-Planck-Institut für Polymerforschung; Alemania
description Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare core–shell silica nanocapsules (SiO2 NCs) via a sol–gel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mL−1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal murine liver cells significantly reduces the release of inflammatory cytokines, indicating an effective suppression of inflammatory response of liver macrophages. Fluorescent and magnetic labeling of the NCs allows for monitoring their intracellular trafficking and biodegradation. Controlled interaction with blood proteins and good colloidal stability in blood plasma are achieved via PEGylation of the NCs. Specific proteins responsible for stealth effect, such as apolipoprotein A-I, apolipoprotein A-IV, and clusterin, are present in large amounts on the PEGylated NCs. In vivo biodistribution investigations prove an efficient accumulation of NCs in the liver, underlining the suitability of the SiO2 NCs as a dexamethasone carrier for treating inflammatory liver diseases.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111896
Jiang, Shuai; Prozeller, Domenik; Pereira, Jorge; Simon, Johanna; Han, Shen; et al.; Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules; Royal Society of Chemistry; Nanoscale; 12; 4; 1-2020; 2626-2637
2040-3364
2040-3372
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111896
identifier_str_mv Jiang, Shuai; Prozeller, Domenik; Pereira, Jorge; Simon, Johanna; Han, Shen; et al.; Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules; Royal Society of Chemistry; Nanoscale; 12; 4; 1-2020; 2626-2637
2040-3364
2040-3372
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2020/NR/C9NR09879H
info:eu-repo/semantics/altIdentifier/doi/10.1039/c9nr09879h
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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