The immunopotentiating effects of shark-derived protein hydrolysate.

Autores
Mallet, Jean-Francois; Duarte, Jairo; Vinderola, Celso Gabriel; Anquenot, Raphael; Beauliau, Martin; Matar, Chantal
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peptides derived from natural sources can act as immunomodulating agents and prevent infections. The aim of this study was to investigate the immunopotentiating and protective effects of a shark-derived protein hydrolysate (SPH) against an enterotoxigenic Escherichia coli H10407 infection in a murine model. METHODS: Mice were fed an aqueous solution of SPH for 7 days before being inoculated with an experimental enterotoxigenic Escherichia coli H10407 infection. After euthanasia, small intestines were removed for histological study and the number of IgA and IgG producing cells was determined by direct immunofluorescence. Cytokines were measured in the serum and the intestinal fluid. RESULTS: The oral administration of SPH enhanced the gut barrier function via up-regulation of immunoglobulin A-producing cells and intestinal cytokines production, including interleukin-6 and tumor necrosis factor-α. The increase of transforming growth factor-β and interleukin-10 contribute to the down-regulation of uncontrolled-inflammatory reaction induced by E. coli infection. From these results, the anti-inflammatory properties of SPH may be caused by regulation and priming mechanisms of the immune system. CONCLUSION: Enzymatic protein degradation confers immunomodulating and protective potentials to shark proteins and the resulted peptides could be used as an alternative therapy to reduce the risk of bacterial infections and inflammatory-related diseases.
Fil: Mallet, Jean-Francois. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; Canadá
Fil: Duarte, Jairo. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; Canadá
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Anquenot, Raphael. InnoVactiv Inc.; Canadá
Fil: Beauliau, Martin. InnoVactiv Inc.; Canadá
Fil: Matar, Chantal. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; Canadá
Materia
Shark-Derives Protein Hydrolysate
Immunomodulation
Intestinal Mucosa
Enterotoxigenic E. Coli Infection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13332
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13332
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The immunopotentiating effects of shark-derived protein hydrolysate.Mallet, Jean-FrancoisDuarte, JairoVinderola, Celso GabrielAnquenot, RaphaelBeauliau, MartinMatar, ChantalShark-Derives Protein HydrolysateImmunomodulationIntestinal MucosaEnterotoxigenic E. Coli Infectionhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Peptides derived from natural sources can act as immunomodulating agents and prevent infections. The aim of this study was to investigate the immunopotentiating and protective effects of a shark-derived protein hydrolysate (SPH) against an enterotoxigenic Escherichia coli H10407 infection in a murine model. METHODS: Mice were fed an aqueous solution of SPH for 7 days before being inoculated with an experimental enterotoxigenic Escherichia coli H10407 infection. After euthanasia, small intestines were removed for histological study and the number of IgA and IgG producing cells was determined by direct immunofluorescence. Cytokines were measured in the serum and the intestinal fluid. RESULTS: The oral administration of SPH enhanced the gut barrier function via up-regulation of immunoglobulin A-producing cells and intestinal cytokines production, including interleukin-6 and tumor necrosis factor-α. The increase of transforming growth factor-β and interleukin-10 contribute to the down-regulation of uncontrolled-inflammatory reaction induced by E. coli infection. From these results, the anti-inflammatory properties of SPH may be caused by regulation and priming mechanisms of the immune system. CONCLUSION: Enzymatic protein degradation confers immunomodulating and protective potentials to shark proteins and the resulted peptides could be used as an alternative therapy to reduce the risk of bacterial infections and inflammatory-related diseases.Fil: Mallet, Jean-Francois. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; CanadáFil: Duarte, Jairo. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; CanadáFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; ArgentinaFil: Anquenot, Raphael. InnoVactiv Inc.; CanadáFil: Beauliau, Martin. InnoVactiv Inc.; CanadáFil: Matar, Chantal. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; CanadáElsevier Science Inc2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13332Mallet, Jean-Francois; Duarte, Jairo; Vinderola, Celso Gabriel; Anquenot, Raphael; Beauliau, Martin; et al.; The immunopotentiating effects of shark-derived protein hydrolysate.; Elsevier Science Inc; Nutrition; 30; 6-2014; 706-7120899-9007enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2013.10.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:00Zoai:ri.conicet.gov.ar:11336/13332instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:00.42CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The immunopotentiating effects of shark-derived protein hydrolysate.
title The immunopotentiating effects of shark-derived protein hydrolysate.
spellingShingle The immunopotentiating effects of shark-derived protein hydrolysate.
Mallet, Jean-Francois
Shark-Derives Protein Hydrolysate
Immunomodulation
Intestinal Mucosa
Enterotoxigenic E. Coli Infection
title_short The immunopotentiating effects of shark-derived protein hydrolysate.
title_full The immunopotentiating effects of shark-derived protein hydrolysate.
title_fullStr The immunopotentiating effects of shark-derived protein hydrolysate.
title_full_unstemmed The immunopotentiating effects of shark-derived protein hydrolysate.
title_sort The immunopotentiating effects of shark-derived protein hydrolysate.
dc.creator.none.fl_str_mv Mallet, Jean-Francois
Duarte, Jairo
Vinderola, Celso Gabriel
Anquenot, Raphael
Beauliau, Martin
Matar, Chantal
author Mallet, Jean-Francois
author_facet Mallet, Jean-Francois
Duarte, Jairo
Vinderola, Celso Gabriel
Anquenot, Raphael
Beauliau, Martin
Matar, Chantal
author_role author
author2 Duarte, Jairo
Vinderola, Celso Gabriel
Anquenot, Raphael
Beauliau, Martin
Matar, Chantal
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Shark-Derives Protein Hydrolysate
Immunomodulation
Intestinal Mucosa
Enterotoxigenic E. Coli Infection
topic Shark-Derives Protein Hydrolysate
Immunomodulation
Intestinal Mucosa
Enterotoxigenic E. Coli Infection
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Peptides derived from natural sources can act as immunomodulating agents and prevent infections. The aim of this study was to investigate the immunopotentiating and protective effects of a shark-derived protein hydrolysate (SPH) against an enterotoxigenic Escherichia coli H10407 infection in a murine model. METHODS: Mice were fed an aqueous solution of SPH for 7 days before being inoculated with an experimental enterotoxigenic Escherichia coli H10407 infection. After euthanasia, small intestines were removed for histological study and the number of IgA and IgG producing cells was determined by direct immunofluorescence. Cytokines were measured in the serum and the intestinal fluid. RESULTS: The oral administration of SPH enhanced the gut barrier function via up-regulation of immunoglobulin A-producing cells and intestinal cytokines production, including interleukin-6 and tumor necrosis factor-α. The increase of transforming growth factor-β and interleukin-10 contribute to the down-regulation of uncontrolled-inflammatory reaction induced by E. coli infection. From these results, the anti-inflammatory properties of SPH may be caused by regulation and priming mechanisms of the immune system. CONCLUSION: Enzymatic protein degradation confers immunomodulating and protective potentials to shark proteins and the resulted peptides could be used as an alternative therapy to reduce the risk of bacterial infections and inflammatory-related diseases.
Fil: Mallet, Jean-Francois. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; Canadá
Fil: Duarte, Jairo. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; Canadá
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Anquenot, Raphael. InnoVactiv Inc.; Canadá
Fil: Beauliau, Martin. InnoVactiv Inc.; Canadá
Fil: Matar, Chantal. University of Ottawa. Faculty of Healt Sciences. Nutrition Sciences Program; Canadá
description Peptides derived from natural sources can act as immunomodulating agents and prevent infections. The aim of this study was to investigate the immunopotentiating and protective effects of a shark-derived protein hydrolysate (SPH) against an enterotoxigenic Escherichia coli H10407 infection in a murine model. METHODS: Mice were fed an aqueous solution of SPH for 7 days before being inoculated with an experimental enterotoxigenic Escherichia coli H10407 infection. After euthanasia, small intestines were removed for histological study and the number of IgA and IgG producing cells was determined by direct immunofluorescence. Cytokines were measured in the serum and the intestinal fluid. RESULTS: The oral administration of SPH enhanced the gut barrier function via up-regulation of immunoglobulin A-producing cells and intestinal cytokines production, including interleukin-6 and tumor necrosis factor-α. The increase of transforming growth factor-β and interleukin-10 contribute to the down-regulation of uncontrolled-inflammatory reaction induced by E. coli infection. From these results, the anti-inflammatory properties of SPH may be caused by regulation and priming mechanisms of the immune system. CONCLUSION: Enzymatic protein degradation confers immunomodulating and protective potentials to shark proteins and the resulted peptides could be used as an alternative therapy to reduce the risk of bacterial infections and inflammatory-related diseases.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13332
Mallet, Jean-Francois; Duarte, Jairo; Vinderola, Celso Gabriel; Anquenot, Raphael; Beauliau, Martin; et al.; The immunopotentiating effects of shark-derived protein hydrolysate.; Elsevier Science Inc; Nutrition; 30; 6-2014; 706-712
0899-9007
url http://hdl.handle.net/11336/13332
identifier_str_mv Mallet, Jean-Francois; Duarte, Jairo; Vinderola, Celso Gabriel; Anquenot, Raphael; Beauliau, Martin; et al.; The immunopotentiating effects of shark-derived protein hydrolysate.; Elsevier Science Inc; Nutrition; 30; 6-2014; 706-712
0899-9007
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2013.10.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613731342876672
score 13.070432

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