A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)

Autores
De Benedictis, Paola; Anderson, Tara C.; Perez, Andres Maximiliano; Viale, Elisabetta; Veggiato, Crispina; Caenazzo, Silvia Tiozzo; Crawford, P. Cynda; Capua, Ilaria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since 2004, there have been several reports of Influenza A virus (FLUAV) infection in dogs. Dogs have been infected with equine influenza H3N8, avian influenza H3N2 and H5N1, and the pandemic H1N1 virus. Because of recent avian and equine influenza outbreaks in Italy, the objectives of the present study were to estimate the level of exposure of Italian dogs to influenza A viruses and to assess a diagnostic algorithm for detection of FLUAV exposure in dogs. Sera collected from 6,858 dogs from 2006 to 2008 were screened in a competitive enzyme-linked immunosorbent assay (cELISA) for antibodies to the highly conserved influenza A nucleoprotein. Samples positive in the cELISA were confirmed by testing in hemagglutination inhibition (HI) and fluorescent antibody test (FAT). Two seropositive dogs had antibodies to H3 hemagglutinin proteins, consistent with exposure to recent canine and equine subtype H3N8 viruses. Using a Bayesian model, the sensitivity and specificity of the cELISA were estimated as 93.98% (probability intervals [PI]: 81.67–99.08%) and 98.71% (PI: 98.43–98.96%), respectively. After accounting for the imperfect sensitivity and specificity of the cELISA, the Bayesian posterior prevalence of FLUAV exposure among tested Italian dogs was 0.5% (PI: 0.1–1.4%). The study results indicate that screening with a cELISA for influenza A nucleoprotein antibody, followed by confirmatory testing with HI and/or FAT, is a highly sensitive and highly specific approach for diagnosing FLUAV exposure in dogs.
Fil: De Benedictis, Paola. Food and Agriculture Organization; Italia
Fil: Anderson, Tara C.. No especifíca;
Fil: Perez, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California; Estados Unidos
Fil: Viale, Elisabetta. Food and Agriculture Organization; Italia
Fil: Veggiato, Crispina. Food and Agriculture Organization; Italia
Fil: Caenazzo, Silvia Tiozzo. Food and Agriculture Organization; Italia
Fil: Crawford, P. Cynda. University of Florida; Estados Unidos
Fil: Capua, Ilaria. Food and Agriculture Organization; Italia
Materia
influenza
dogs
Italy
surveillance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242654
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242654
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)De Benedictis, PaolaAnderson, Tara C.Perez, Andres MaximilianoViale, ElisabettaVeggiato, CrispinaCaenazzo, Silvia TiozzoCrawford, P. CyndaCapua, IlariainfluenzadogsItalysurveillancehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Since 2004, there have been several reports of Influenza A virus (FLUAV) infection in dogs. Dogs have been infected with equine influenza H3N8, avian influenza H3N2 and H5N1, and the pandemic H1N1 virus. Because of recent avian and equine influenza outbreaks in Italy, the objectives of the present study were to estimate the level of exposure of Italian dogs to influenza A viruses and to assess a diagnostic algorithm for detection of FLUAV exposure in dogs. Sera collected from 6,858 dogs from 2006 to 2008 were screened in a competitive enzyme-linked immunosorbent assay (cELISA) for antibodies to the highly conserved influenza A nucleoprotein. Samples positive in the cELISA were confirmed by testing in hemagglutination inhibition (HI) and fluorescent antibody test (FAT). Two seropositive dogs had antibodies to H3 hemagglutinin proteins, consistent with exposure to recent canine and equine subtype H3N8 viruses. Using a Bayesian model, the sensitivity and specificity of the cELISA were estimated as 93.98% (probability intervals [PI]: 81.67–99.08%) and 98.71% (PI: 98.43–98.96%), respectively. After accounting for the imperfect sensitivity and specificity of the cELISA, the Bayesian posterior prevalence of FLUAV exposure among tested Italian dogs was 0.5% (PI: 0.1–1.4%). The study results indicate that screening with a cELISA for influenza A nucleoprotein antibody, followed by confirmatory testing with HI and/or FAT, is a highly sensitive and highly specific approach for diagnosing FLUAV exposure in dogs.Fil: De Benedictis, Paola. Food and Agriculture Organization; ItaliaFil: Anderson, Tara C.. No especifíca;Fil: Perez, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California; Estados UnidosFil: Viale, Elisabetta. Food and Agriculture Organization; ItaliaFil: Veggiato, Crispina. Food and Agriculture Organization; ItaliaFil: Caenazzo, Silvia Tiozzo. Food and Agriculture Organization; ItaliaFil: Crawford, P. Cynda. University of Florida; Estados UnidosFil: Capua, Ilaria. Food and Agriculture Organization; ItaliaAmerican Association of Veterinary Laboratory Diagnosticians2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242654De Benedictis, Paola; Anderson, Tara C.; Perez, Andres Maximiliano; Viale, Elisabetta; Veggiato, Crispina; et al.; A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008); American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 22; 6; 3-2010; 914-9201040-6387CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/104063871002200610info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/104063871002200610#tab-contributorsinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:12Zoai:ri.conicet.gov.ar:11336/242654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:13.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
title A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
spellingShingle A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
De Benedictis, Paola
influenza
dogs
Italy
surveillance
title_short A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
title_full A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
title_fullStr A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
title_full_unstemmed A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
title_sort A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)
dc.creator.none.fl_str_mv De Benedictis, Paola
Anderson, Tara C.
Perez, Andres Maximiliano
Viale, Elisabetta
Veggiato, Crispina
Caenazzo, Silvia Tiozzo
Crawford, P. Cynda
Capua, Ilaria
author De Benedictis, Paola
author_facet De Benedictis, Paola
Anderson, Tara C.
Perez, Andres Maximiliano
Viale, Elisabetta
Veggiato, Crispina
Caenazzo, Silvia Tiozzo
Crawford, P. Cynda
Capua, Ilaria
author_role author
author2 Anderson, Tara C.
Perez, Andres Maximiliano
Viale, Elisabetta
Veggiato, Crispina
Caenazzo, Silvia Tiozzo
Crawford, P. Cynda
Capua, Ilaria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv influenza
dogs
Italy
surveillance
topic influenza
dogs
Italy
surveillance
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Since 2004, there have been several reports of Influenza A virus (FLUAV) infection in dogs. Dogs have been infected with equine influenza H3N8, avian influenza H3N2 and H5N1, and the pandemic H1N1 virus. Because of recent avian and equine influenza outbreaks in Italy, the objectives of the present study were to estimate the level of exposure of Italian dogs to influenza A viruses and to assess a diagnostic algorithm for detection of FLUAV exposure in dogs. Sera collected from 6,858 dogs from 2006 to 2008 were screened in a competitive enzyme-linked immunosorbent assay (cELISA) for antibodies to the highly conserved influenza A nucleoprotein. Samples positive in the cELISA were confirmed by testing in hemagglutination inhibition (HI) and fluorescent antibody test (FAT). Two seropositive dogs had antibodies to H3 hemagglutinin proteins, consistent with exposure to recent canine and equine subtype H3N8 viruses. Using a Bayesian model, the sensitivity and specificity of the cELISA were estimated as 93.98% (probability intervals [PI]: 81.67–99.08%) and 98.71% (PI: 98.43–98.96%), respectively. After accounting for the imperfect sensitivity and specificity of the cELISA, the Bayesian posterior prevalence of FLUAV exposure among tested Italian dogs was 0.5% (PI: 0.1–1.4%). The study results indicate that screening with a cELISA for influenza A nucleoprotein antibody, followed by confirmatory testing with HI and/or FAT, is a highly sensitive and highly specific approach for diagnosing FLUAV exposure in dogs.
Fil: De Benedictis, Paola. Food and Agriculture Organization; Italia
Fil: Anderson, Tara C.. No especifíca;
Fil: Perez, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California; Estados Unidos
Fil: Viale, Elisabetta. Food and Agriculture Organization; Italia
Fil: Veggiato, Crispina. Food and Agriculture Organization; Italia
Fil: Caenazzo, Silvia Tiozzo. Food and Agriculture Organization; Italia
Fil: Crawford, P. Cynda. University of Florida; Estados Unidos
Fil: Capua, Ilaria. Food and Agriculture Organization; Italia
description Since 2004, there have been several reports of Influenza A virus (FLUAV) infection in dogs. Dogs have been infected with equine influenza H3N8, avian influenza H3N2 and H5N1, and the pandemic H1N1 virus. Because of recent avian and equine influenza outbreaks in Italy, the objectives of the present study were to estimate the level of exposure of Italian dogs to influenza A viruses and to assess a diagnostic algorithm for detection of FLUAV exposure in dogs. Sera collected from 6,858 dogs from 2006 to 2008 were screened in a competitive enzyme-linked immunosorbent assay (cELISA) for antibodies to the highly conserved influenza A nucleoprotein. Samples positive in the cELISA were confirmed by testing in hemagglutination inhibition (HI) and fluorescent antibody test (FAT). Two seropositive dogs had antibodies to H3 hemagglutinin proteins, consistent with exposure to recent canine and equine subtype H3N8 viruses. Using a Bayesian model, the sensitivity and specificity of the cELISA were estimated as 93.98% (probability intervals [PI]: 81.67–99.08%) and 98.71% (PI: 98.43–98.96%), respectively. After accounting for the imperfect sensitivity and specificity of the cELISA, the Bayesian posterior prevalence of FLUAV exposure among tested Italian dogs was 0.5% (PI: 0.1–1.4%). The study results indicate that screening with a cELISA for influenza A nucleoprotein antibody, followed by confirmatory testing with HI and/or FAT, is a highly sensitive and highly specific approach for diagnosing FLUAV exposure in dogs.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242654
De Benedictis, Paola; Anderson, Tara C.; Perez, Andres Maximiliano; Viale, Elisabetta; Veggiato, Crispina; et al.; A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008); American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 22; 6; 3-2010; 914-920
1040-6387
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242654
identifier_str_mv De Benedictis, Paola; Anderson, Tara C.; Perez, Andres Maximiliano; Viale, Elisabetta; Veggiato, Crispina; et al.; A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008); American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 22; 6; 3-2010; 914-920
1040-6387
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/104063871002200610
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/104063871002200610#tab-contributors
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082574619770880
score 13.22299

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