Curcumin effects on Leydig cell functions and potential therapeutic uses

Autores
Raices, Trinidad; Varela, María Luisa; Abiuso, Adriana María Belén; Pereyra, Elba Nora; Mondillo, Carolina; Pignataro, Omar Pedro; Riera, Maria Fernanda
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCTs). Due to their steroid-secreting nature, LCTs entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin?s impact on Leydig cells? functions and its potential effect on LCT growth. In vitro assays on MA-10 Leydig cells showed that curcumin (20?80 µmol/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin?s in vitro cytostatic capacity, we show that 40?80 µmol/L curcumin reduces MA-10 Leydig cells? proliferative capacity, which could be explained by the arrest in G2/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received i.p. injections of 20 mg/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin?s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence of curcumin?s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.
Fil: Raices, Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Varela, María Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Abiuso, Adriana María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pereyra, Elba Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Mondillo, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pignataro, Omar Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
Materia
LEYDIG CELL
CURCUMIN
STEROIDOGENESIS
NATURAL THERAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215933
Acceder
id CONICETDig_ab064fd86cca30c78e9d145597497444
oai_identifier_str oai:ri.conicet.gov.ar:11336/215933
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Curcumin effects on Leydig cell functions and potential therapeutic usesRaices, TrinidadVarela, María LuisaAbiuso, Adriana María BelénPereyra, Elba NoraMondillo, CarolinaPignataro, Omar PedroRiera, Maria FernandaLEYDIG CELLCURCUMINSTEROIDOGENESISNATURAL THERAPHYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCTs). Due to their steroid-secreting nature, LCTs entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin?s impact on Leydig cells? functions and its potential effect on LCT growth. In vitro assays on MA-10 Leydig cells showed that curcumin (20?80 µmol/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin?s in vitro cytostatic capacity, we show that 40?80 µmol/L curcumin reduces MA-10 Leydig cells? proliferative capacity, which could be explained by the arrest in G2/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received i.p. injections of 20 mg/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin?s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence of curcumin?s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.Fil: Raices, Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Varela, María Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Abiuso, Adriana María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pereyra, Elba Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Mondillo, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pignataro, Omar Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaSociety for Endocrinolohgy2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215933Raices, Trinidad; Varela, María Luisa; Abiuso, Adriana María Belén; Pereyra, Elba Nora; Mondillo, Carolina; et al.; Curcumin effects on Leydig cell functions and potential therapeutic uses; Society for Endocrinolohgy; Endocrine Oncology; 3; 1; 2-2023; 1-132673-396X2634-4793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://eo.bioscientifica.com/view/journals/eo/3/1/EO-22-0075.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1530/EO-22-0075info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:43Zoai:ri.conicet.gov.ar:11336/215933instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:43.835CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Curcumin effects on Leydig cell functions and potential therapeutic uses
title Curcumin effects on Leydig cell functions and potential therapeutic uses
spellingShingle Curcumin effects on Leydig cell functions and potential therapeutic uses
Raices, Trinidad
LEYDIG CELL
CURCUMIN
STEROIDOGENESIS
NATURAL THERAPHY
title_short Curcumin effects on Leydig cell functions and potential therapeutic uses
title_full Curcumin effects on Leydig cell functions and potential therapeutic uses
title_fullStr Curcumin effects on Leydig cell functions and potential therapeutic uses
title_full_unstemmed Curcumin effects on Leydig cell functions and potential therapeutic uses
title_sort Curcumin effects on Leydig cell functions and potential therapeutic uses
dc.creator.none.fl_str_mv Raices, Trinidad
Varela, María Luisa
Abiuso, Adriana María Belén
Pereyra, Elba Nora
Mondillo, Carolina
Pignataro, Omar Pedro
Riera, Maria Fernanda
author Raices, Trinidad
author_facet Raices, Trinidad
Varela, María Luisa
Abiuso, Adriana María Belén
Pereyra, Elba Nora
Mondillo, Carolina
Pignataro, Omar Pedro
Riera, Maria Fernanda
author_role author
author2 Varela, María Luisa
Abiuso, Adriana María Belén
Pereyra, Elba Nora
Mondillo, Carolina
Pignataro, Omar Pedro
Riera, Maria Fernanda
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv LEYDIG CELL
CURCUMIN
STEROIDOGENESIS
NATURAL THERAPHY
topic LEYDIG CELL
CURCUMIN
STEROIDOGENESIS
NATURAL THERAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCTs). Due to their steroid-secreting nature, LCTs entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin?s impact on Leydig cells? functions and its potential effect on LCT growth. In vitro assays on MA-10 Leydig cells showed that curcumin (20?80 µmol/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin?s in vitro cytostatic capacity, we show that 40?80 µmol/L curcumin reduces MA-10 Leydig cells? proliferative capacity, which could be explained by the arrest in G2/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received i.p. injections of 20 mg/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin?s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence of curcumin?s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.
Fil: Raices, Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Varela, María Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Abiuso, Adriana María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pereyra, Elba Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Mondillo, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pignataro, Omar Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
description Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCTs). Due to their steroid-secreting nature, LCTs entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin?s impact on Leydig cells? functions and its potential effect on LCT growth. In vitro assays on MA-10 Leydig cells showed that curcumin (20?80 µmol/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin?s in vitro cytostatic capacity, we show that 40?80 µmol/L curcumin reduces MA-10 Leydig cells? proliferative capacity, which could be explained by the arrest in G2/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received i.p. injections of 20 mg/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin?s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence of curcumin?s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215933
Raices, Trinidad; Varela, María Luisa; Abiuso, Adriana María Belén; Pereyra, Elba Nora; Mondillo, Carolina; et al.; Curcumin effects on Leydig cell functions and potential therapeutic uses; Society for Endocrinolohgy; Endocrine Oncology; 3; 1; 2-2023; 1-13
2673-396X
2634-4793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215933
identifier_str_mv Raices, Trinidad; Varela, María Luisa; Abiuso, Adriana María Belén; Pereyra, Elba Nora; Mondillo, Carolina; et al.; Curcumin effects on Leydig cell functions and potential therapeutic uses; Society for Endocrinolohgy; Endocrine Oncology; 3; 1; 2-2023; 1-13
2673-396X
2634-4793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://eo.bioscientifica.com/view/journals/eo/3/1/EO-22-0075.xml
info:eu-repo/semantics/altIdentifier/doi/10.1530/EO-22-0075
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Endocrinolohgy
publisher.none.fl_str_mv Society for Endocrinolohgy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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