Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile

Autores
Nina, Nélida; Quispe, Cristina; Jiménez Aspee, Felipe; Theoduloz, Cristina; Feresin, Gabriela Egly; Lima, Beatriz Viviana; Leiva, Elba; Schmeda Hirschmann, Guillermo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Propolis is commercialized in Chile as an antimicrobial agent. It is obtained mainly from central and southern Chile, but is used for the same purposes regardless of its origin. To compare the antimicrobial effect, the total phenolic (TP), the total flavonoid (TF) content and the phenolic composition, 19 samples were collected in the main production centers in the Región del Maule, Chile. Samples were extracted with MeOH and assessed for antimicrobial activity against Gram (+) and Gram (−) bacteria. TP and TF content, antioxidant activity by the DPPH, FRAP and TEAC methods were also determined. Sample composition was assessed by HPLD-DAD-ESI-MS/MS. Differential compounds in the samples were isolated and characterized. The antimicrobial effect of the samples showed MICs ranging from 31.5 to > 1000 μg/mL. Propolis from the central valley was more effective as antibacterial than those from the coastal area or Andean slopes. The samples considered of interest (MIC ≤ 62.5 μg/mL) showed effect on Escherichia coli, Pseudomonas sp., Yersinia enterocolitica and Salmonella enteritidis. Two new diarylheptanoids, a diterpene, the flavonoids pinocembrin and chrysin were isolated and elucidated by spectroscopic and spectrometric means. Some 29 compounds were dereplicated by HPLC-MS and tentatively identified, including nine flavones/flavonol derivatives, one flavanone, eight dihydroflavonols and nine phenyl-propanoids. Propolis from the Región del Maule showed large variation in antimicrobial effect, antioxidant activity and composition. So far the presence of diarylheptanoids in samples from the coastal area of central Chile can be considered as a marker of a new type of propolis.
Fil: Nina, Nélida. Universidad de Talca; Chile
Fil: Quispe, Cristina. Universidad de Talca; Chile. Universidad Arturo Prat; Chile
Fil: Jiménez Aspee, Felipe. Universidad de Talca; Chile
Fil: Theoduloz, Cristina. Universidad de Talca; Chile
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leiva, Elba. Universidad de Talca; Chile
Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca; Chile
Materia
PROPOLIS
ANTIMICROBIAL
PHENOLICS
FLAVONOIDS
DIARYLHEPTANOIDS
POILACNEIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42162
Acceder
id CONICETDig_aa98b52463a406077a47901f412317af
oai_identifier_str oai:ri.conicet.gov.ar:11336/42162
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central ChileNina, NélidaQuispe, CristinaJiménez Aspee, FelipeTheoduloz, CristinaFeresin, Gabriela EglyLima, Beatriz VivianaLeiva, ElbaSchmeda Hirschmann, GuillermoPROPOLISANTIMICROBIALPHENOLICSFLAVONOIDSDIARYLHEPTANOIDSPOILACNEIC ACIDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Propolis is commercialized in Chile as an antimicrobial agent. It is obtained mainly from central and southern Chile, but is used for the same purposes regardless of its origin. To compare the antimicrobial effect, the total phenolic (TP), the total flavonoid (TF) content and the phenolic composition, 19 samples were collected in the main production centers in the Región del Maule, Chile. Samples were extracted with MeOH and assessed for antimicrobial activity against Gram (+) and Gram (−) bacteria. TP and TF content, antioxidant activity by the DPPH, FRAP and TEAC methods were also determined. Sample composition was assessed by HPLD-DAD-ESI-MS/MS. Differential compounds in the samples were isolated and characterized. The antimicrobial effect of the samples showed MICs ranging from 31.5 to > 1000 μg/mL. Propolis from the central valley was more effective as antibacterial than those from the coastal area or Andean slopes. The samples considered of interest (MIC ≤ 62.5 μg/mL) showed effect on Escherichia coli, Pseudomonas sp., Yersinia enterocolitica and Salmonella enteritidis. Two new diarylheptanoids, a diterpene, the flavonoids pinocembrin and chrysin were isolated and elucidated by spectroscopic and spectrometric means. Some 29 compounds were dereplicated by HPLC-MS and tentatively identified, including nine flavones/flavonol derivatives, one flavanone, eight dihydroflavonols and nine phenyl-propanoids. Propolis from the Región del Maule showed large variation in antimicrobial effect, antioxidant activity and composition. So far the presence of diarylheptanoids in samples from the coastal area of central Chile can be considered as a marker of a new type of propolis.Fil: Nina, Nélida. Universidad de Talca; ChileFil: Quispe, Cristina. Universidad de Talca; Chile. Universidad Arturo Prat; ChileFil: Jiménez Aspee, Felipe. Universidad de Talca; ChileFil: Theoduloz, Cristina. Universidad de Talca; ChileFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leiva, Elba. Universidad de Talca; ChileFil: Schmeda Hirschmann, Guillermo. Universidad de Talca; ChileMolecular Diversity Preservation International2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42162Nina, Nélida; Quispe, Cristina; Jiménez Aspee, Felipe; Theoduloz, Cristina ; Feresin, Gabriela Egly; et al.; Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile; Molecular Diversity Preservation International; Molecules; 20; 10; 10-2015; 18144-181671420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/molecules201018144info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/20/10/18144info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:40Zoai:ri.conicet.gov.ar:11336/42162instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:40.411CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
title Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
spellingShingle Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
Nina, Nélida
PROPOLIS
ANTIMICROBIAL
PHENOLICS
FLAVONOIDS
DIARYLHEPTANOIDS
POILACNEIC ACID
title_short Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
title_full Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
title_fullStr Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
title_full_unstemmed Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
title_sort Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile
dc.creator.none.fl_str_mv Nina, Nélida
Quispe, Cristina
Jiménez Aspee, Felipe
Theoduloz, Cristina
Feresin, Gabriela Egly
Lima, Beatriz Viviana
Leiva, Elba
Schmeda Hirschmann, Guillermo
author Nina, Nélida
author_facet Nina, Nélida
Quispe, Cristina
Jiménez Aspee, Felipe
Theoduloz, Cristina
Feresin, Gabriela Egly
Lima, Beatriz Viviana
Leiva, Elba
Schmeda Hirschmann, Guillermo
author_role author
author2 Quispe, Cristina
Jiménez Aspee, Felipe
Theoduloz, Cristina
Feresin, Gabriela Egly
Lima, Beatriz Viviana
Leiva, Elba
Schmeda Hirschmann, Guillermo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROPOLIS
ANTIMICROBIAL
PHENOLICS
FLAVONOIDS
DIARYLHEPTANOIDS
POILACNEIC ACID
topic PROPOLIS
ANTIMICROBIAL
PHENOLICS
FLAVONOIDS
DIARYLHEPTANOIDS
POILACNEIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Propolis is commercialized in Chile as an antimicrobial agent. It is obtained mainly from central and southern Chile, but is used for the same purposes regardless of its origin. To compare the antimicrobial effect, the total phenolic (TP), the total flavonoid (TF) content and the phenolic composition, 19 samples were collected in the main production centers in the Región del Maule, Chile. Samples were extracted with MeOH and assessed for antimicrobial activity against Gram (+) and Gram (−) bacteria. TP and TF content, antioxidant activity by the DPPH, FRAP and TEAC methods were also determined. Sample composition was assessed by HPLD-DAD-ESI-MS/MS. Differential compounds in the samples were isolated and characterized. The antimicrobial effect of the samples showed MICs ranging from 31.5 to > 1000 μg/mL. Propolis from the central valley was more effective as antibacterial than those from the coastal area or Andean slopes. The samples considered of interest (MIC ≤ 62.5 μg/mL) showed effect on Escherichia coli, Pseudomonas sp., Yersinia enterocolitica and Salmonella enteritidis. Two new diarylheptanoids, a diterpene, the flavonoids pinocembrin and chrysin were isolated and elucidated by spectroscopic and spectrometric means. Some 29 compounds were dereplicated by HPLC-MS and tentatively identified, including nine flavones/flavonol derivatives, one flavanone, eight dihydroflavonols and nine phenyl-propanoids. Propolis from the Región del Maule showed large variation in antimicrobial effect, antioxidant activity and composition. So far the presence of diarylheptanoids in samples from the coastal area of central Chile can be considered as a marker of a new type of propolis.
Fil: Nina, Nélida. Universidad de Talca; Chile
Fil: Quispe, Cristina. Universidad de Talca; Chile. Universidad Arturo Prat; Chile
Fil: Jiménez Aspee, Felipe. Universidad de Talca; Chile
Fil: Theoduloz, Cristina. Universidad de Talca; Chile
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leiva, Elba. Universidad de Talca; Chile
Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca; Chile
description Propolis is commercialized in Chile as an antimicrobial agent. It is obtained mainly from central and southern Chile, but is used for the same purposes regardless of its origin. To compare the antimicrobial effect, the total phenolic (TP), the total flavonoid (TF) content and the phenolic composition, 19 samples were collected in the main production centers in the Región del Maule, Chile. Samples were extracted with MeOH and assessed for antimicrobial activity against Gram (+) and Gram (−) bacteria. TP and TF content, antioxidant activity by the DPPH, FRAP and TEAC methods were also determined. Sample composition was assessed by HPLD-DAD-ESI-MS/MS. Differential compounds in the samples were isolated and characterized. The antimicrobial effect of the samples showed MICs ranging from 31.5 to > 1000 μg/mL. Propolis from the central valley was more effective as antibacterial than those from the coastal area or Andean slopes. The samples considered of interest (MIC ≤ 62.5 μg/mL) showed effect on Escherichia coli, Pseudomonas sp., Yersinia enterocolitica and Salmonella enteritidis. Two new diarylheptanoids, a diterpene, the flavonoids pinocembrin and chrysin were isolated and elucidated by spectroscopic and spectrometric means. Some 29 compounds were dereplicated by HPLC-MS and tentatively identified, including nine flavones/flavonol derivatives, one flavanone, eight dihydroflavonols and nine phenyl-propanoids. Propolis from the Región del Maule showed large variation in antimicrobial effect, antioxidant activity and composition. So far the presence of diarylheptanoids in samples from the coastal area of central Chile can be considered as a marker of a new type of propolis.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42162
Nina, Nélida; Quispe, Cristina; Jiménez Aspee, Felipe; Theoduloz, Cristina ; Feresin, Gabriela Egly; et al.; Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile; Molecular Diversity Preservation International; Molecules; 20; 10; 10-2015; 18144-18167
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42162
identifier_str_mv Nina, Nélida; Quispe, Cristina; Jiménez Aspee, Felipe; Theoduloz, Cristina ; Feresin, Gabriela Egly; et al.; Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Región del Maule, Central Chile; Molecular Diversity Preservation International; Molecules; 20; 10; 10-2015; 18144-18167
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules201018144
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/20/10/18144
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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