GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes

Autores
Guerra Liliana; Suárez, Cecilia Ana; Soto, Daniela; Schiappacasse, Agustina; Sapochnik, Daiana; Sacca, Paula Alejandra; Piwien Pilipuk, Graciela; Peral, Belen; Calvo, Juan Carlos
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
IntroductionIn the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal?epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124?131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells.Materials and methodsConditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.ResultsWe described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.ConclusionsTaken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.
Fil: Guerra Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Suárez, Cecilia Ana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Computación; Argentina
Fil: Soto, Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Schiappacasse, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sapochnik, Daiana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Laboratorio de Proteoglicanos y Matriz extracelular; Argentina
Fil: Piwien Pilipuk, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Peral, Belen. Universidad Autónoma de Madrid; España
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Laboratorio de Proteoglicanos y Matriz Extracelular; Argentina
Materia
Adipocytes
Breast Mammary Gland
Triglycerides
Cancer
Cell Lines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8265
Acceder
id CONICETDig_aa973cbd16201c925f65a92394aaef81
oai_identifier_str oai:ri.conicet.gov.ar:11336/8265
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytesGuerra LilianaSuárez, Cecilia AnaSoto, DanielaSchiappacasse, AgustinaSapochnik, DaianaSacca, Paula AlejandraPiwien Pilipuk, GracielaPeral, BelenCalvo, Juan CarlosAdipocytesBreast Mammary GlandTriglyceridesCancerCell Lineshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1IntroductionIn the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal?epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124?131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells.Materials and methodsConditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.ResultsWe described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.ConclusionsTaken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.Fil: Guerra Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Suárez, Cecilia Ana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Computación; ArgentinaFil: Soto, Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Schiappacasse, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sapochnik, Daiana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Laboratorio de Proteoglicanos y Matriz extracelular; ArgentinaFil: Piwien Pilipuk, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Peral, Belen. Universidad Autónoma de Madrid; EspañaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Laboratorio de Proteoglicanos y Matriz Extracelular; ArgentinaSpringer2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8265Guerra Liliana; Suárez, Cecilia Ana; Soto, Daniela; Schiappacasse, Agustina; Sapochnik, Daiana; et al.; GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes; Springer; Clinical & Translational Oncology; 17; 7; 12-2014; 511-5201699-048Xenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s12094-014-1267-6info:eu-repo/semantics/altIdentifier/doi/10.1007/s12094-014-1267-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:17:08Zoai:ri.conicet.gov.ar:11336/8265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:17:08.85CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
title GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
spellingShingle GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
Guerra Liliana
Adipocytes
Breast Mammary Gland
Triglycerides
Cancer
Cell Lines
title_short GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
title_full GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
title_fullStr GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
title_full_unstemmed GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
title_sort GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
dc.creator.none.fl_str_mv Guerra Liliana
Suárez, Cecilia Ana
Soto, Daniela
Schiappacasse, Agustina
Sapochnik, Daiana
Sacca, Paula Alejandra
Piwien Pilipuk, Graciela
Peral, Belen
Calvo, Juan Carlos
author Guerra Liliana
author_facet Guerra Liliana
Suárez, Cecilia Ana
Soto, Daniela
Schiappacasse, Agustina
Sapochnik, Daiana
Sacca, Paula Alejandra
Piwien Pilipuk, Graciela
Peral, Belen
Calvo, Juan Carlos
author_role author
author2 Suárez, Cecilia Ana
Soto, Daniela
Schiappacasse, Agustina
Sapochnik, Daiana
Sacca, Paula Alejandra
Piwien Pilipuk, Graciela
Peral, Belen
Calvo, Juan Carlos
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adipocytes
Breast Mammary Gland
Triglycerides
Cancer
Cell Lines
topic Adipocytes
Breast Mammary Gland
Triglycerides
Cancer
Cell Lines
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv IntroductionIn the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal?epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124?131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells.Materials and methodsConditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.ResultsWe described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.ConclusionsTaken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.
Fil: Guerra Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Suárez, Cecilia Ana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Computación; Argentina
Fil: Soto, Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Schiappacasse, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sapochnik, Daiana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Laboratorio de Proteoglicanos y Matriz extracelular; Argentina
Fil: Piwien Pilipuk, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Peral, Belen. Universidad Autónoma de Madrid; España
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Laboratorio de Proteoglicanos y Matriz Extracelular; Argentina
description IntroductionIn the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal?epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124?131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells.Materials and methodsConditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.ResultsWe described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.ConclusionsTaken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8265
Guerra Liliana; Suárez, Cecilia Ana; Soto, Daniela; Schiappacasse, Agustina; Sapochnik, Daiana; et al.; GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes; Springer; Clinical & Translational Oncology; 17; 7; 12-2014; 511-520
1699-048X
url http://hdl.handle.net/11336/8265
identifier_str_mv Guerra Liliana; Suárez, Cecilia Ana; Soto, Daniela; Schiappacasse, Agustina; Sapochnik, Daiana; et al.; GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes; Springer; Clinical & Translational Oncology; 17; 7; 12-2014; 511-520
1699-048X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s12094-014-1267-6
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12094-014-1267-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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