SRAP as an informative molecular marker to study the Fusarium Poae genetic variability

Autores
Dinolfo, María Inés; Castañares, Eliana; Stenglein, Sebastian Alberto
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium poae is one of the Fusarium species isolated from grains associated with Fusarium Head Blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using sequence-related amplified polymorphism (SRAP) to analyze the capacity of this molecular marker to evaluate the F. poae genetic variability. The molecular analysis showed high intraspecific variability within F. poae isolates and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different Fusarium species showing that the SRAP technique not only allows studying F. poae genetic variability, but also targets coding regions into the F. poae genome. To our knowledge this is the first report on genetic variability of F. poae using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus.
Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina
Fil: Castañares, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina
Materia
Fusarium Poae
Genetic Variability
Orfs
Sequence-Related Amplified Polimorphism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23849
Acceder
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network_name_str CONICET Digital (CONICET)
spelling SRAP as an informative molecular marker to study the Fusarium Poae genetic variabilityDinolfo, María InésCastañares, ElianaStenglein, Sebastian AlbertoFusarium PoaeGenetic VariabilityOrfsSequence-Related Amplified Polimorphismhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Fusarium poae is one of the Fusarium species isolated from grains associated with Fusarium Head Blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using sequence-related amplified polymorphism (SRAP) to analyze the capacity of this molecular marker to evaluate the F. poae genetic variability. The molecular analysis showed high intraspecific variability within F. poae isolates and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different Fusarium species showing that the SRAP technique not only allows studying F. poae genetic variability, but also targets coding regions into the F. poae genome. To our knowledge this is the first report on genetic variability of F. poae using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus.Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; ArgentinaFil: Castañares, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; ArgentinaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; ArgentinaWiley2015-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23849Dinolfo, María Inés; Castañares, Eliana; Stenglein, Sebastian Alberto; SRAP as an informative molecular marker to study the Fusarium Poae genetic variability; Wiley; Journal of Phytopathology-Phytopathologische Zeitschrift; 163; 7-8; 29-7-2015; 657-6630931-1785CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jph.12301/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/jph.12301info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:21Zoai:ri.conicet.gov.ar:11336/23849instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:21.351CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
title SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
spellingShingle SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
Dinolfo, María Inés
Fusarium Poae
Genetic Variability
Orfs
Sequence-Related Amplified Polimorphism
title_short SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
title_full SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
title_fullStr SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
title_full_unstemmed SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
title_sort SRAP as an informative molecular marker to study the Fusarium Poae genetic variability
dc.creator.none.fl_str_mv Dinolfo, María Inés
Castañares, Eliana
Stenglein, Sebastian Alberto
author Dinolfo, María Inés
author_facet Dinolfo, María Inés
Castañares, Eliana
Stenglein, Sebastian Alberto
author_role author
author2 Castañares, Eliana
Stenglein, Sebastian Alberto
author2_role author
author
dc.subject.none.fl_str_mv Fusarium Poae
Genetic Variability
Orfs
Sequence-Related Amplified Polimorphism
topic Fusarium Poae
Genetic Variability
Orfs
Sequence-Related Amplified Polimorphism
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fusarium poae is one of the Fusarium species isolated from grains associated with Fusarium Head Blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using sequence-related amplified polymorphism (SRAP) to analyze the capacity of this molecular marker to evaluate the F. poae genetic variability. The molecular analysis showed high intraspecific variability within F. poae isolates and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different Fusarium species showing that the SRAP technique not only allows studying F. poae genetic variability, but also targets coding regions into the F. poae genome. To our knowledge this is the first report on genetic variability of F. poae using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus.
Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina
Fil: Castañares, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina
description Fusarium poae is one of the Fusarium species isolated from grains associated with Fusarium Head Blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using sequence-related amplified polymorphism (SRAP) to analyze the capacity of this molecular marker to evaluate the F. poae genetic variability. The molecular analysis showed high intraspecific variability within F. poae isolates and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different Fusarium species showing that the SRAP technique not only allows studying F. poae genetic variability, but also targets coding regions into the F. poae genome. To our knowledge this is the first report on genetic variability of F. poae using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23849
Dinolfo, María Inés; Castañares, Eliana; Stenglein, Sebastian Alberto; SRAP as an informative molecular marker to study the Fusarium Poae genetic variability; Wiley; Journal of Phytopathology-Phytopathologische Zeitschrift; 163; 7-8; 29-7-2015; 657-663
0931-1785
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23849
identifier_str_mv Dinolfo, María Inés; Castañares, Eliana; Stenglein, Sebastian Alberto; SRAP as an informative molecular marker to study the Fusarium Poae genetic variability; Wiley; Journal of Phytopathology-Phytopathologische Zeitschrift; 163; 7-8; 29-7-2015; 657-663
0931-1785
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jph.12301/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/jph.12301
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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