Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products

Autores
Sampietro, Diego Alejandro; Jiménez, Cristina Marisol; Belizán, María Melina Eleonora; Vattuone, Marta Amelia; Catalan, Cesar Atilio Nazareno
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A 96-well plate micromethod was developed to measure 5-n-alkylresorcinols (5nARs) in cereal grains and food derived products. The 5nARs reacted in alkaline alcoholic medium with Fast Blue RR ½ZnCl2 salt to yield coloured azo-derivatives. The highest sensitivity for 5nARs was obtained at 490 nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. This reaction showed good linearity for olivetol (0.05–0.20 μg). Contents of 5nARs determined in cereal grains and derived products by the new Fast Blue RR micromethod were highly correlated (R2 = 0.9944) with those obtained by a Fast Blue B method currently used. A Bland–Altman analysis indicated a small positive bias near to zero (R2 = 0.0401), suggesting that the methods can be interchangeably used. The new reaction is completed in 15 min and the coloured products are read within the 15 min after completion. The micromethod offers a fast analysis of 5nARs in cereal grains and derived products with low consumption of reagents and solvents.
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Jiménez, Cristina Marisol. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Belizán, María Melina Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Materia
Alkylresorcinols
Bran
Whole-Grain Products
Fast Blue Rr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7165
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain productsSampietro, Diego AlejandroJiménez, Cristina MarisolBelizán, María Melina EleonoraVattuone, Marta AmeliaCatalan, Cesar Atilio NazarenoAlkylresorcinolsBranWhole-Grain ProductsFast Blue Rrhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4A 96-well plate micromethod was developed to measure 5-n-alkylresorcinols (5nARs) in cereal grains and food derived products. The 5nARs reacted in alkaline alcoholic medium with Fast Blue RR ½ZnCl2 salt to yield coloured azo-derivatives. The highest sensitivity for 5nARs was obtained at 490 nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. This reaction showed good linearity for olivetol (0.05–0.20 μg). Contents of 5nARs determined in cereal grains and derived products by the new Fast Blue RR micromethod were highly correlated (R2 = 0.9944) with those obtained by a Fast Blue B method currently used. A Bland–Altman analysis indicated a small positive bias near to zero (R2 = 0.0401), suggesting that the methods can be interchangeably used. The new reaction is completed in 15 min and the coloured products are read within the 15 min after completion. The micromethod offers a fast analysis of 5nARs in cereal grains and derived products with low consumption of reagents and solvents.Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Jiménez, Cristina Marisol. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Belizán, María Melina Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaElsevier2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7165Sampietro, Diego Alejandro; Jiménez, Cristina Marisol; Belizán, María Melina Eleonora; Vattuone, Marta Amelia; Catalan, Cesar Atilio Nazareno; Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products; Elsevier; Food Chemistry; 141; 4; 12-2013; 3546-35510308-8146enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814613008467info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2013.06.069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:58Zoai:ri.conicet.gov.ar:11336/7165instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:59.178CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
title Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
spellingShingle Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
Sampietro, Diego Alejandro
Alkylresorcinols
Bran
Whole-Grain Products
Fast Blue Rr
title_short Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
title_full Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
title_fullStr Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
title_full_unstemmed Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
title_sort Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products
dc.creator.none.fl_str_mv Sampietro, Diego Alejandro
Jiménez, Cristina Marisol
Belizán, María Melina Eleonora
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
author Sampietro, Diego Alejandro
author_facet Sampietro, Diego Alejandro
Jiménez, Cristina Marisol
Belizán, María Melina Eleonora
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
author_role author
author2 Jiménez, Cristina Marisol
Belizán, María Melina Eleonora
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
author2_role author
author
author
author
dc.subject.none.fl_str_mv Alkylresorcinols
Bran
Whole-Grain Products
Fast Blue Rr
topic Alkylresorcinols
Bran
Whole-Grain Products
Fast Blue Rr
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A 96-well plate micromethod was developed to measure 5-n-alkylresorcinols (5nARs) in cereal grains and food derived products. The 5nARs reacted in alkaline alcoholic medium with Fast Blue RR ½ZnCl2 salt to yield coloured azo-derivatives. The highest sensitivity for 5nARs was obtained at 490 nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. This reaction showed good linearity for olivetol (0.05–0.20 μg). Contents of 5nARs determined in cereal grains and derived products by the new Fast Blue RR micromethod were highly correlated (R2 = 0.9944) with those obtained by a Fast Blue B method currently used. A Bland–Altman analysis indicated a small positive bias near to zero (R2 = 0.0401), suggesting that the methods can be interchangeably used. The new reaction is completed in 15 min and the coloured products are read within the 15 min after completion. The micromethod offers a fast analysis of 5nARs in cereal grains and derived products with low consumption of reagents and solvents.
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Jiménez, Cristina Marisol. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Belizán, María Melina Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
description A 96-well plate micromethod was developed to measure 5-n-alkylresorcinols (5nARs) in cereal grains and food derived products. The 5nARs reacted in alkaline alcoholic medium with Fast Blue RR ½ZnCl2 salt to yield coloured azo-derivatives. The highest sensitivity for 5nARs was obtained at 490 nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. This reaction showed good linearity for olivetol (0.05–0.20 μg). Contents of 5nARs determined in cereal grains and derived products by the new Fast Blue RR micromethod were highly correlated (R2 = 0.9944) with those obtained by a Fast Blue B method currently used. A Bland–Altman analysis indicated a small positive bias near to zero (R2 = 0.0401), suggesting that the methods can be interchangeably used. The new reaction is completed in 15 min and the coloured products are read within the 15 min after completion. The micromethod offers a fast analysis of 5nARs in cereal grains and derived products with low consumption of reagents and solvents.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7165
Sampietro, Diego Alejandro; Jiménez, Cristina Marisol; Belizán, María Melina Eleonora; Vattuone, Marta Amelia; Catalan, Cesar Atilio Nazareno; Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products; Elsevier; Food Chemistry; 141; 4; 12-2013; 3546-3551
0308-8146
url http://hdl.handle.net/11336/7165
identifier_str_mv Sampietro, Diego Alejandro; Jiménez, Cristina Marisol; Belizán, María Melina Eleonora; Vattuone, Marta Amelia; Catalan, Cesar Atilio Nazareno; Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products; Elsevier; Food Chemistry; 141; 4; 12-2013; 3546-3551
0308-8146
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814613008467
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2013.06.069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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