Chromosomal alterations in atherosclerotic plaques

Autores
Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Lavezzi, Anna Maria; Vandone, Pier Luigi; Gabrielli, Livio; Fernández Alonso, Graciela; Grana, Daniel Rodolfo; Milei, Jose
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alterations of chromosomes 7 and 11 have been involved in the progression of atherosclerosis. Twenty-three carotid endarterectomy specimens were studied for the presence of alterations in chromosomes 7 and 11, and fibroblastic growth factor-3 (FGF-3) gene amplification. Besides classic histological stainings, immunophenotyping of cellular and vascular components and fluorescence in situ hybridization (FISH) were performed. At the caps, unstable plaques (n = 18) showed inflammatory infiltration of macrophages, smooth muscle cells, and T-lymphocytes. Specifically in these regions, the FISH showed varying percentages of trisomy (15/18) and tetrasomy (8/15) of chromosome 7. In four cases polisomy 7 was noted in some nuclei. Monosomy of chromosome 11 and gene amplification of FGF-3 gene was observed. The FISH of the five stable plaques and normal arterial walls showed no chromosome alterations; furthermore, chromosome 3, which is not involved in atherosclerotic progression, presented a normal ploidy of smooth muscle cells in stable and unstable plaques and normal arterial walls. In conclusion, chromosome 7 and 11 alterations and FGF-3 gene amplification are components of unstable plaques, and might contribute to the evolution of stable plaques into complicated plaques.
Fil: Matturri, Luigi. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Cazzullo, Alessandra. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Turconi, Paola. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Lavezzi, Anna Maria. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Vandone, Pier Luigi. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Gabrielli, Livio. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Fernández Alonso, Graciela. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Materia
ATHEROSCLEROSIS
CAROTID ARTERY
CHROMOSOMAL ALTERATIONS
CHROMOSOME 11
CHROMOSOME 7
FIBROBLASTIC GROWTH FACTOR-3, (FGF-3)
GENETIC ALTERATIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/186633
Acceder
id CONICETDig_a9e109213b4c3c38af540696a4d186ad
oai_identifier_str oai:ri.conicet.gov.ar:11336/186633
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chromosomal alterations in atherosclerotic plaquesMatturri, LuigiCazzullo, AlessandraTurconi, PaolaLavezzi, Anna MariaVandone, Pier LuigiGabrielli, LivioFernández Alonso, GracielaGrana, Daniel RodolfoMilei, JoseATHEROSCLEROSISCAROTID ARTERYCHROMOSOMAL ALTERATIONSCHROMOSOME 11CHROMOSOME 7FIBROBLASTIC GROWTH FACTOR-3, (FGF-3)GENETIC ALTERATIONShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Alterations of chromosomes 7 and 11 have been involved in the progression of atherosclerosis. Twenty-three carotid endarterectomy specimens were studied for the presence of alterations in chromosomes 7 and 11, and fibroblastic growth factor-3 (FGF-3) gene amplification. Besides classic histological stainings, immunophenotyping of cellular and vascular components and fluorescence in situ hybridization (FISH) were performed. At the caps, unstable plaques (n = 18) showed inflammatory infiltration of macrophages, smooth muscle cells, and T-lymphocytes. Specifically in these regions, the FISH showed varying percentages of trisomy (15/18) and tetrasomy (8/15) of chromosome 7. In four cases polisomy 7 was noted in some nuclei. Monosomy of chromosome 11 and gene amplification of FGF-3 gene was observed. The FISH of the five stable plaques and normal arterial walls showed no chromosome alterations; furthermore, chromosome 3, which is not involved in atherosclerotic progression, presented a normal ploidy of smooth muscle cells in stable and unstable plaques and normal arterial walls. In conclusion, chromosome 7 and 11 alterations and FGF-3 gene amplification are components of unstable plaques, and might contribute to the evolution of stable plaques into complicated plaques.Fil: Matturri, Luigi. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Cazzullo, Alessandra. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Turconi, Paola. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Lavezzi, Anna Maria. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Vandone, Pier Luigi. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Gabrielli, Livio. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Fernández Alonso, Graciela. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaElsevier Ireland2001-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/186633Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Lavezzi, Anna Maria; Vandone, Pier Luigi; et al.; Chromosomal alterations in atherosclerotic plaques; Elsevier Ireland; Atherosclerosis; 154; 3; 2-2001; 755-7610021-9150CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021915000004883?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0021-9150(00)00488-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:59Zoai:ri.conicet.gov.ar:11336/186633instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:59.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chromosomal alterations in atherosclerotic plaques
title Chromosomal alterations in atherosclerotic plaques
spellingShingle Chromosomal alterations in atherosclerotic plaques
Matturri, Luigi
ATHEROSCLEROSIS
CAROTID ARTERY
CHROMOSOMAL ALTERATIONS
CHROMOSOME 11
CHROMOSOME 7
FIBROBLASTIC GROWTH FACTOR-3, (FGF-3)
GENETIC ALTERATIONS
title_short Chromosomal alterations in atherosclerotic plaques
title_full Chromosomal alterations in atherosclerotic plaques
title_fullStr Chromosomal alterations in atherosclerotic plaques
title_full_unstemmed Chromosomal alterations in atherosclerotic plaques
title_sort Chromosomal alterations in atherosclerotic plaques
dc.creator.none.fl_str_mv Matturri, Luigi
Cazzullo, Alessandra
Turconi, Paola
Lavezzi, Anna Maria
Vandone, Pier Luigi
Gabrielli, Livio
Fernández Alonso, Graciela
Grana, Daniel Rodolfo
Milei, Jose
author Matturri, Luigi
author_facet Matturri, Luigi
Cazzullo, Alessandra
Turconi, Paola
Lavezzi, Anna Maria
Vandone, Pier Luigi
Gabrielli, Livio
Fernández Alonso, Graciela
Grana, Daniel Rodolfo
Milei, Jose
author_role author
author2 Cazzullo, Alessandra
Turconi, Paola
Lavezzi, Anna Maria
Vandone, Pier Luigi
Gabrielli, Livio
Fernández Alonso, Graciela
Grana, Daniel Rodolfo
Milei, Jose
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ATHEROSCLEROSIS
CAROTID ARTERY
CHROMOSOMAL ALTERATIONS
CHROMOSOME 11
CHROMOSOME 7
FIBROBLASTIC GROWTH FACTOR-3, (FGF-3)
GENETIC ALTERATIONS
topic ATHEROSCLEROSIS
CAROTID ARTERY
CHROMOSOMAL ALTERATIONS
CHROMOSOME 11
CHROMOSOME 7
FIBROBLASTIC GROWTH FACTOR-3, (FGF-3)
GENETIC ALTERATIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Alterations of chromosomes 7 and 11 have been involved in the progression of atherosclerosis. Twenty-three carotid endarterectomy specimens were studied for the presence of alterations in chromosomes 7 and 11, and fibroblastic growth factor-3 (FGF-3) gene amplification. Besides classic histological stainings, immunophenotyping of cellular and vascular components and fluorescence in situ hybridization (FISH) were performed. At the caps, unstable plaques (n = 18) showed inflammatory infiltration of macrophages, smooth muscle cells, and T-lymphocytes. Specifically in these regions, the FISH showed varying percentages of trisomy (15/18) and tetrasomy (8/15) of chromosome 7. In four cases polisomy 7 was noted in some nuclei. Monosomy of chromosome 11 and gene amplification of FGF-3 gene was observed. The FISH of the five stable plaques and normal arterial walls showed no chromosome alterations; furthermore, chromosome 3, which is not involved in atherosclerotic progression, presented a normal ploidy of smooth muscle cells in stable and unstable plaques and normal arterial walls. In conclusion, chromosome 7 and 11 alterations and FGF-3 gene amplification are components of unstable plaques, and might contribute to the evolution of stable plaques into complicated plaques.
Fil: Matturri, Luigi. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Cazzullo, Alessandra. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Turconi, Paola. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Lavezzi, Anna Maria. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Vandone, Pier Luigi. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Gabrielli, Livio. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Fernández Alonso, Graciela. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
description Alterations of chromosomes 7 and 11 have been involved in the progression of atherosclerosis. Twenty-three carotid endarterectomy specimens were studied for the presence of alterations in chromosomes 7 and 11, and fibroblastic growth factor-3 (FGF-3) gene amplification. Besides classic histological stainings, immunophenotyping of cellular and vascular components and fluorescence in situ hybridization (FISH) were performed. At the caps, unstable plaques (n = 18) showed inflammatory infiltration of macrophages, smooth muscle cells, and T-lymphocytes. Specifically in these regions, the FISH showed varying percentages of trisomy (15/18) and tetrasomy (8/15) of chromosome 7. In four cases polisomy 7 was noted in some nuclei. Monosomy of chromosome 11 and gene amplification of FGF-3 gene was observed. The FISH of the five stable plaques and normal arterial walls showed no chromosome alterations; furthermore, chromosome 3, which is not involved in atherosclerotic progression, presented a normal ploidy of smooth muscle cells in stable and unstable plaques and normal arterial walls. In conclusion, chromosome 7 and 11 alterations and FGF-3 gene amplification are components of unstable plaques, and might contribute to the evolution of stable plaques into complicated plaques.
publishDate 2001
dc.date.none.fl_str_mv 2001-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/186633
Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Lavezzi, Anna Maria; Vandone, Pier Luigi; et al.; Chromosomal alterations in atherosclerotic plaques; Elsevier Ireland; Atherosclerosis; 154; 3; 2-2001; 755-761
0021-9150
CONICET Digital
CONICET
url http://hdl.handle.net/11336/186633
identifier_str_mv Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Lavezzi, Anna Maria; Vandone, Pier Luigi; et al.; Chromosomal alterations in atherosclerotic plaques; Elsevier Ireland; Atherosclerosis; 154; 3; 2-2001; 755-761
0021-9150
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021915000004883?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0021-9150(00)00488-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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