Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis

Autores
Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Roncoroni, Lucia; Grana, Daniel Rodolfo; Milei, Jose
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlamydia pneumoniae (CP), chromosomal alterations and apoptosis were suggested as contributing factors in the pathogenesis of atherosclerosis. Early (EP) and unstable plaques (UP) were studied in order to assess infiltrate composition, the apoptotic index, chromosome 7 stability and to investigate the concurrent presence of CP in EP and UP. Paraffin embedded sections of three iliac arteries and four aortas from young donors (EP), and four coronaries and nine carotid arteries (UP) were used. Aside from histological techniques, immunophenotypification for macrophages, T and B cells, smooth muscle and endothelial cells; FISH and DNA nick end labeling were performed. The amplifications with PCR for CP infection were negative in all specimens. In the EP, a focal myointimal thickening with foam cells and scarce smooth muscle cells was observed. Macrophages were most frequent in the intima (10.8%) while T and B cells were found in 2.3 and 1.5%. In the UP a thin cap covering a lipid-rich core with widespread vascularization and with severe luminal obstruction was observed. Macrophages were increased (21%), and T (1.5%) and B cells (3.5%) in the caps and inner areas of the lipid cores. At these sites, the FISH showed trisomy and tetrasomy of chromosome 7 and apoptosis was very frequent (10-30%). Macrophages in intimal lesions is one of the most prominent, consistent and permanent features in EP, and an elevated apoptotic index and chromosome 7 instability might contribute to evolution from stable to complicated plaques, while CP seems to play no role. However, further studies are needed with more cases to confirm this last observation. Copyright (C) 2000 Elsevier Science Ireland Ltd.
Fil: Matturri, Luigi. Università degli Studi di Milano; Italia. IRCCS Ospedale Maggiore; Italia
Fil: Cazzullo, Alessandra. Università degli Studi di Milano; Italia. IRCCS Ospedale Maggiore; Italia
Fil: Turconi, Paola. IRCCS Ospedale Maggiore; Italia. Università degli Studi di Milano; Italia
Fil: Roncoroni, Lucia. IRCCS Ospedale Maggiore; Italia. Università degli Studi di Milano; Italia
Fil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador. Facultad de Medicina; Argentina
Fil: Milei, Jose. Universidad del Salvador. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Materia
Apoptosis
Atherosclerosis
Chlamydia Pneumoniae
Chromosome 7
Macrophage
Smooth Muscle Cell
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39105
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosisMatturri, LuigiCazzullo, AlessandraTurconi, PaolaRoncoroni, LuciaGrana, Daniel RodolfoMilei, JoseApoptosisAtherosclerosisChlamydia PneumoniaeChromosome 7MacrophageSmooth Muscle Cellhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Chlamydia pneumoniae (CP), chromosomal alterations and apoptosis were suggested as contributing factors in the pathogenesis of atherosclerosis. Early (EP) and unstable plaques (UP) were studied in order to assess infiltrate composition, the apoptotic index, chromosome 7 stability and to investigate the concurrent presence of CP in EP and UP. Paraffin embedded sections of three iliac arteries and four aortas from young donors (EP), and four coronaries and nine carotid arteries (UP) were used. Aside from histological techniques, immunophenotypification for macrophages, T and B cells, smooth muscle and endothelial cells; FISH and DNA nick end labeling were performed. The amplifications with PCR for CP infection were negative in all specimens. In the EP, a focal myointimal thickening with foam cells and scarce smooth muscle cells was observed. Macrophages were most frequent in the intima (10.8%) while T and B cells were found in 2.3 and 1.5%. In the UP a thin cap covering a lipid-rich core with widespread vascularization and with severe luminal obstruction was observed. Macrophages were increased (21%), and T (1.5%) and B cells (3.5%) in the caps and inner areas of the lipid cores. At these sites, the FISH showed trisomy and tetrasomy of chromosome 7 and apoptosis was very frequent (10-30%). Macrophages in intimal lesions is one of the most prominent, consistent and permanent features in EP, and an elevated apoptotic index and chromosome 7 instability might contribute to evolution from stable to complicated plaques, while CP seems to play no role. However, further studies are needed with more cases to confirm this last observation. Copyright (C) 2000 Elsevier Science Ireland Ltd.Fil: Matturri, Luigi. Università degli Studi di Milano; Italia. IRCCS Ospedale Maggiore; ItaliaFil: Cazzullo, Alessandra. Università degli Studi di Milano; Italia. IRCCS Ospedale Maggiore; ItaliaFil: Turconi, Paola. IRCCS Ospedale Maggiore; Italia. Università degli Studi di Milano; ItaliaFil: Roncoroni, Lucia. IRCCS Ospedale Maggiore; Italia. Università degli Studi di Milano; ItaliaFil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador. Facultad de Medicina; ArgentinaFil: Milei, Jose. Universidad del Salvador. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaElsevier Ireland2000-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/39105Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Roncoroni, Lucia; Grana, Daniel Rodolfo; et al.; Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis; Elsevier Ireland; International Journal of Cardiology; 75; 1; 8-2000; 23-330167-5273CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167527300003065info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-5273(00)00306-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:56Zoai:ri.conicet.gov.ar:11336/39105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:56.493CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
title Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
spellingShingle Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
Matturri, Luigi
Apoptosis
Atherosclerosis
Chlamydia Pneumoniae
Chromosome 7
Macrophage
Smooth Muscle Cell
title_short Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
title_full Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
title_fullStr Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
title_full_unstemmed Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
title_sort Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis
dc.creator.none.fl_str_mv Matturri, Luigi
Cazzullo, Alessandra
Turconi, Paola
Roncoroni, Lucia
Grana, Daniel Rodolfo
Milei, Jose
author Matturri, Luigi
author_facet Matturri, Luigi
Cazzullo, Alessandra
Turconi, Paola
Roncoroni, Lucia
Grana, Daniel Rodolfo
Milei, Jose
author_role author
author2 Cazzullo, Alessandra
Turconi, Paola
Roncoroni, Lucia
Grana, Daniel Rodolfo
Milei, Jose
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Atherosclerosis
Chlamydia Pneumoniae
Chromosome 7
Macrophage
Smooth Muscle Cell
topic Apoptosis
Atherosclerosis
Chlamydia Pneumoniae
Chromosome 7
Macrophage
Smooth Muscle Cell
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chlamydia pneumoniae (CP), chromosomal alterations and apoptosis were suggested as contributing factors in the pathogenesis of atherosclerosis. Early (EP) and unstable plaques (UP) were studied in order to assess infiltrate composition, the apoptotic index, chromosome 7 stability and to investigate the concurrent presence of CP in EP and UP. Paraffin embedded sections of three iliac arteries and four aortas from young donors (EP), and four coronaries and nine carotid arteries (UP) were used. Aside from histological techniques, immunophenotypification for macrophages, T and B cells, smooth muscle and endothelial cells; FISH and DNA nick end labeling were performed. The amplifications with PCR for CP infection were negative in all specimens. In the EP, a focal myointimal thickening with foam cells and scarce smooth muscle cells was observed. Macrophages were most frequent in the intima (10.8%) while T and B cells were found in 2.3 and 1.5%. In the UP a thin cap covering a lipid-rich core with widespread vascularization and with severe luminal obstruction was observed. Macrophages were increased (21%), and T (1.5%) and B cells (3.5%) in the caps and inner areas of the lipid cores. At these sites, the FISH showed trisomy and tetrasomy of chromosome 7 and apoptosis was very frequent (10-30%). Macrophages in intimal lesions is one of the most prominent, consistent and permanent features in EP, and an elevated apoptotic index and chromosome 7 instability might contribute to evolution from stable to complicated plaques, while CP seems to play no role. However, further studies are needed with more cases to confirm this last observation. Copyright (C) 2000 Elsevier Science Ireland Ltd.
Fil: Matturri, Luigi. Università degli Studi di Milano; Italia. IRCCS Ospedale Maggiore; Italia
Fil: Cazzullo, Alessandra. Università degli Studi di Milano; Italia. IRCCS Ospedale Maggiore; Italia
Fil: Turconi, Paola. IRCCS Ospedale Maggiore; Italia. Università degli Studi di Milano; Italia
Fil: Roncoroni, Lucia. IRCCS Ospedale Maggiore; Italia. Università degli Studi di Milano; Italia
Fil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador. Facultad de Medicina; Argentina
Fil: Milei, Jose. Universidad del Salvador. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
description Chlamydia pneumoniae (CP), chromosomal alterations and apoptosis were suggested as contributing factors in the pathogenesis of atherosclerosis. Early (EP) and unstable plaques (UP) were studied in order to assess infiltrate composition, the apoptotic index, chromosome 7 stability and to investigate the concurrent presence of CP in EP and UP. Paraffin embedded sections of three iliac arteries and four aortas from young donors (EP), and four coronaries and nine carotid arteries (UP) were used. Aside from histological techniques, immunophenotypification for macrophages, T and B cells, smooth muscle and endothelial cells; FISH and DNA nick end labeling were performed. The amplifications with PCR for CP infection were negative in all specimens. In the EP, a focal myointimal thickening with foam cells and scarce smooth muscle cells was observed. Macrophages were most frequent in the intima (10.8%) while T and B cells were found in 2.3 and 1.5%. In the UP a thin cap covering a lipid-rich core with widespread vascularization and with severe luminal obstruction was observed. Macrophages were increased (21%), and T (1.5%) and B cells (3.5%) in the caps and inner areas of the lipid cores. At these sites, the FISH showed trisomy and tetrasomy of chromosome 7 and apoptosis was very frequent (10-30%). Macrophages in intimal lesions is one of the most prominent, consistent and permanent features in EP, and an elevated apoptotic index and chromosome 7 instability might contribute to evolution from stable to complicated plaques, while CP seems to play no role. However, further studies are needed with more cases to confirm this last observation. Copyright (C) 2000 Elsevier Science Ireland Ltd.
publishDate 2000
dc.date.none.fl_str_mv 2000-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39105
Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Roncoroni, Lucia; Grana, Daniel Rodolfo; et al.; Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis; Elsevier Ireland; International Journal of Cardiology; 75; 1; 8-2000; 23-33
0167-5273
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39105
identifier_str_mv Matturri, Luigi; Cazzullo, Alessandra; Turconi, Paola; Roncoroni, Lucia; Grana, Daniel Rodolfo; et al.; Inflammatory cells, apoptosis and Chlamydia pneumoniae infection in atherosclerosis; Elsevier Ireland; International Journal of Cardiology; 75; 1; 8-2000; 23-33
0167-5273
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167527300003065
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-5273(00)00306-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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