Effect of estrogen estrone on uterine and adipose tissue under obesity

Autores
Valle, María Ivone; Cepeda, Sabrina Belén; Cutini, Pablo Hernan; Sandoval, Marisa Julia; Massheimer, Virginia Laura
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Previously we reported that, estrone (E1) counteracts uterine oxidative stress induced by an inflammatory environment. In this work, using an experimental model of obesity, the direct action of E1 on uterus (Ut) and adipose tissue (AT) was evaluated. In order to rule out the influence of the hormonal impact during estrous cycle, animals were bilaterally ovariectomized (OVX). Rats were fed with high-fat diet (OVX-Ob) for 10 weeks. After isolation, Ut and retroperitoneal AT explants were in vitro expose to 10 nM E1. Histological analysis of Ut explants (H&E staining) showed that in non-OVX rats exhibited a thicker endometrial layer with great amount of uterine glands compared to uterus sections from OVX rats, verifying the absence of estrous cycles in OVX group. The serum biochemical evaluation showed that, Ob rats exhibited higher levels of serum H2O2; TBARS, and leptin than non-obese (8; 65; 122% above non-Ob, respectively, p< 0.05), profile compatible with obesity. After E1 treatment (4h), Ut slices exhibited an enhancement in nitric oxide synthesis (33% above control P<0.001) and reduction in ROS production (30% below control, P<0.01). To assess whether the effect of E1 on H2O2 generation depends on its ability to enhance NO synthesis, an irreversible NOS inhibitor (L-NAME) was employed. In the presence of L-NAME, the reduction on ROS production elicited by E1 was blocked, suggesting NO dependence. Indeed, in OVX-Ob rats the steroid treatment stimulates uterus angiogenesis. Concomitantly, on AT explants isolated from OVX-Ob rats, E1 prompted an antioxidant action. E1 reduced H202 secretion (2375±263 vs 1978±241 nmol/g AT, C vs E1 p<0.05), and TBARS released (502±195 vs 353±30 nmol/g AT, C vs E1 p<0.05). Indeed, a 14% reduction on leptin secretion was also detected (p<0.05). The results presented evidenced that adipose tissue is targeted of E1 action, and that under obesity the hormone exerts a protective action on Ut and AT reducing oxidative stress.
Fil: Valle, María Ivone. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión Anual de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); XXV Annual Conferences of Sociedad Argentina de Biología (SAB); LV Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACYTA)
Mar del Plata
Argentina
Sociedad argentina de investigación clínica
Sociedad argentina de inmunología
Asociación argentina de farmacología experimental
Asociación argentina de ciencia y tecnología de animales de laboratorio
Materia
ESTRONE
UTERINE TISSUE
ADIPOSE TISSUE
OBESITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244492
Acceder
id CONICETDig_a9b142332d7ca04079b5cd04e5410322
oai_identifier_str oai:ri.conicet.gov.ar:11336/244492
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of estrogen estrone on uterine and adipose tissue under obesityValle, María IvoneCepeda, Sabrina BelénCutini, Pablo HernanSandoval, Marisa JuliaMassheimer, Virginia LauraESTRONEUTERINE TISSUEADIPOSE TISSUEOBESITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previously we reported that, estrone (E1) counteracts uterine oxidative stress induced by an inflammatory environment. In this work, using an experimental model of obesity, the direct action of E1 on uterus (Ut) and adipose tissue (AT) was evaluated. In order to rule out the influence of the hormonal impact during estrous cycle, animals were bilaterally ovariectomized (OVX). Rats were fed with high-fat diet (OVX-Ob) for 10 weeks. After isolation, Ut and retroperitoneal AT explants were in vitro expose to 10 nM E1. Histological analysis of Ut explants (H&E staining) showed that in non-OVX rats exhibited a thicker endometrial layer with great amount of uterine glands compared to uterus sections from OVX rats, verifying the absence of estrous cycles in OVX group. The serum biochemical evaluation showed that, Ob rats exhibited higher levels of serum H2O2; TBARS, and leptin than non-obese (8; 65; 122% above non-Ob, respectively, p< 0.05), profile compatible with obesity. After E1 treatment (4h), Ut slices exhibited an enhancement in nitric oxide synthesis (33% above control P<0.001) and reduction in ROS production (30% below control, P<0.01). To assess whether the effect of E1 on H2O2 generation depends on its ability to enhance NO synthesis, an irreversible NOS inhibitor (L-NAME) was employed. In the presence of L-NAME, the reduction on ROS production elicited by E1 was blocked, suggesting NO dependence. Indeed, in OVX-Ob rats the steroid treatment stimulates uterus angiogenesis. Concomitantly, on AT explants isolated from OVX-Ob rats, E1 prompted an antioxidant action. E1 reduced H202 secretion (2375±263 vs 1978±241 nmol/g AT, C vs E1 p<0.05), and TBARS released (502±195 vs 353±30 nmol/g AT, C vs E1 p<0.05). Indeed, a 14% reduction on leptin secretion was also detected (p<0.05). The results presented evidenced that adipose tissue is targeted of E1 action, and that under obesity the hormone exerts a protective action on Ut and AT reducing oxidative stress.Fil: Valle, María Ivone. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaReunión Anual de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); XXV Annual Conferences of Sociedad Argentina de Biología (SAB); LV Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACYTA)Mar del PlataArgentinaSociedad argentina de investigación clínicaSociedad argentina de inmunologíaAsociación argentina de farmacología experimentalAsociación argentina de ciencia y tecnología de animales de laboratorioFundación Revista MedicinaLuthy, Isabel Alicia2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244492Effect of estrogen estrone on uterine and adipose tissue under obesity; Reunión Anual de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); XXV Annual Conferences of Sociedad Argentina de Biología (SAB); LV Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACYTA); Mar del Plata; Argentina; 2023; 103-1031669-9106CONICET DigitalCONICETenghttps://www.saic.org.ar/reuniones-anuales-previasinfo:eu-repo/semantics/altIdentifier/url/http://www.medicinabuenosaires.comNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:43Zoai:ri.conicet.gov.ar:11336/244492instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:44.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of estrogen estrone on uterine and adipose tissue under obesity
title Effect of estrogen estrone on uterine and adipose tissue under obesity
spellingShingle Effect of estrogen estrone on uterine and adipose tissue under obesity
Valle, María Ivone
ESTRONE
UTERINE TISSUE
ADIPOSE TISSUE
OBESITY
title_short Effect of estrogen estrone on uterine and adipose tissue under obesity
title_full Effect of estrogen estrone on uterine and adipose tissue under obesity
title_fullStr Effect of estrogen estrone on uterine and adipose tissue under obesity
title_full_unstemmed Effect of estrogen estrone on uterine and adipose tissue under obesity
title_sort Effect of estrogen estrone on uterine and adipose tissue under obesity
dc.creator.none.fl_str_mv Valle, María Ivone
Cepeda, Sabrina Belén
Cutini, Pablo Hernan
Sandoval, Marisa Julia
Massheimer, Virginia Laura
author Valle, María Ivone
author_facet Valle, María Ivone
Cepeda, Sabrina Belén
Cutini, Pablo Hernan
Sandoval, Marisa Julia
Massheimer, Virginia Laura
author_role author
author2 Cepeda, Sabrina Belén
Cutini, Pablo Hernan
Sandoval, Marisa Julia
Massheimer, Virginia Laura
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Luthy, Isabel Alicia
dc.subject.none.fl_str_mv ESTRONE
UTERINE TISSUE
ADIPOSE TISSUE
OBESITY
topic ESTRONE
UTERINE TISSUE
ADIPOSE TISSUE
OBESITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previously we reported that, estrone (E1) counteracts uterine oxidative stress induced by an inflammatory environment. In this work, using an experimental model of obesity, the direct action of E1 on uterus (Ut) and adipose tissue (AT) was evaluated. In order to rule out the influence of the hormonal impact during estrous cycle, animals were bilaterally ovariectomized (OVX). Rats were fed with high-fat diet (OVX-Ob) for 10 weeks. After isolation, Ut and retroperitoneal AT explants were in vitro expose to 10 nM E1. Histological analysis of Ut explants (H&E staining) showed that in non-OVX rats exhibited a thicker endometrial layer with great amount of uterine glands compared to uterus sections from OVX rats, verifying the absence of estrous cycles in OVX group. The serum biochemical evaluation showed that, Ob rats exhibited higher levels of serum H2O2; TBARS, and leptin than non-obese (8; 65; 122% above non-Ob, respectively, p< 0.05), profile compatible with obesity. After E1 treatment (4h), Ut slices exhibited an enhancement in nitric oxide synthesis (33% above control P<0.001) and reduction in ROS production (30% below control, P<0.01). To assess whether the effect of E1 on H2O2 generation depends on its ability to enhance NO synthesis, an irreversible NOS inhibitor (L-NAME) was employed. In the presence of L-NAME, the reduction on ROS production elicited by E1 was blocked, suggesting NO dependence. Indeed, in OVX-Ob rats the steroid treatment stimulates uterus angiogenesis. Concomitantly, on AT explants isolated from OVX-Ob rats, E1 prompted an antioxidant action. E1 reduced H202 secretion (2375±263 vs 1978±241 nmol/g AT, C vs E1 p<0.05), and TBARS released (502±195 vs 353±30 nmol/g AT, C vs E1 p<0.05). Indeed, a 14% reduction on leptin secretion was also detected (p<0.05). The results presented evidenced that adipose tissue is targeted of E1 action, and that under obesity the hormone exerts a protective action on Ut and AT reducing oxidative stress.
Fil: Valle, María Ivone. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión Anual de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); XXV Annual Conferences of Sociedad Argentina de Biología (SAB); LV Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACYTA)
Mar del Plata
Argentina
Sociedad argentina de investigación clínica
Sociedad argentina de inmunología
Asociación argentina de farmacología experimental
Asociación argentina de ciencia y tecnología de animales de laboratorio
description Previously we reported that, estrone (E1) counteracts uterine oxidative stress induced by an inflammatory environment. In this work, using an experimental model of obesity, the direct action of E1 on uterus (Ut) and adipose tissue (AT) was evaluated. In order to rule out the influence of the hormonal impact during estrous cycle, animals were bilaterally ovariectomized (OVX). Rats were fed with high-fat diet (OVX-Ob) for 10 weeks. After isolation, Ut and retroperitoneal AT explants were in vitro expose to 10 nM E1. Histological analysis of Ut explants (H&E staining) showed that in non-OVX rats exhibited a thicker endometrial layer with great amount of uterine glands compared to uterus sections from OVX rats, verifying the absence of estrous cycles in OVX group. The serum biochemical evaluation showed that, Ob rats exhibited higher levels of serum H2O2; TBARS, and leptin than non-obese (8; 65; 122% above non-Ob, respectively, p< 0.05), profile compatible with obesity. After E1 treatment (4h), Ut slices exhibited an enhancement in nitric oxide synthesis (33% above control P<0.001) and reduction in ROS production (30% below control, P<0.01). To assess whether the effect of E1 on H2O2 generation depends on its ability to enhance NO synthesis, an irreversible NOS inhibitor (L-NAME) was employed. In the presence of L-NAME, the reduction on ROS production elicited by E1 was blocked, suggesting NO dependence. Indeed, in OVX-Ob rats the steroid treatment stimulates uterus angiogenesis. Concomitantly, on AT explants isolated from OVX-Ob rats, E1 prompted an antioxidant action. E1 reduced H202 secretion (2375±263 vs 1978±241 nmol/g AT, C vs E1 p<0.05), and TBARS released (502±195 vs 353±30 nmol/g AT, C vs E1 p<0.05). Indeed, a 14% reduction on leptin secretion was also detected (p<0.05). The results presented evidenced that adipose tissue is targeted of E1 action, and that under obesity the hormone exerts a protective action on Ut and AT reducing oxidative stress.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244492
Effect of estrogen estrone on uterine and adipose tissue under obesity; Reunión Anual de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); XXV Annual Conferences of Sociedad Argentina de Biología (SAB); LV Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACYTA); Mar del Plata; Argentina; 2023; 103-103
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244492
identifier_str_mv Effect of estrogen estrone on uterine and adipose tissue under obesity; Reunión Anual de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); XXV Annual Conferences of Sociedad Argentina de Biología (SAB); LV Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACYTA); Mar del Plata; Argentina; 2023; 103-103
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.saic.org.ar/reuniones-anuales-previas
info:eu-repo/semantics/altIdentifier/url/http://www.medicinabuenosaires.com
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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