Anti-glioma properties of DVL, a lectin purified from Dioclea violacea

Autores
Nascimento, Ana Paula M.; Knaut, Jhônatas L.; Rieger, Débora K.; Wolin, Ingrid A.V.; Heinrich, Isabella A.; Mann, Josiane; Juarez, Andrea Virginia; Sosa, Liliana del Valle; de Paul, Ana Lucia; Moreira, Cleane G.; Silva, Ivanice B.; Nobre, Clareane S.; Osterne, Vinicius J.S.; Nascimento, Kyria S.; Cavada, Benildo S.; Leal, Rodrigo B.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well-studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity.
Fil: Nascimento, Ana Paula M.. Universidade Federal de Santa Catarina; Brasil
Fil: Knaut, Jhônatas L.. Universidade Federal de Santa Catarina; Brasil
Fil: Rieger, Débora K.. Universidade Federal de Santa Catarina; Brasil
Fil: Wolin, Ingrid A.V.. Universidade Federal de Santa Catarina; Brasil
Fil: Heinrich, Isabella A.. Universidade Federal de Santa Catarina; Brasil
Fil: Mann, Josiane. Universidade Federal de Santa Catarina; Brasil
Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; Argentina
Fil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; Argentina
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; Argentina
Fil: Moreira, Cleane G.. Universidade Estadual do Ceará; Brasil
Fil: Silva, Ivanice B.. Universidade Estadual do Ceará; Brasil
Fil: Nobre, Clareane S.. Universidade Estadual do Ceará; Brasil
Fil: Osterne, Vinicius J.S.. Universidade Estadual do Ceará; Brasil
Fil: Nascimento, Kyria S.. Universidade Estadual do Ceará; Brasil
Fil: Cavada, Benildo S.. Universidade Estadual do Ceará; Brasil
Fil: Leal, Rodrigo B.. Universidade Federal de Santa Catarina; Brasil
Materia
AUTOPHAGY
CONA
DIOCLEA VIOLACEA
DVL
GLIOMA
LECTIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91543
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/91543
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-glioma properties of DVL, a lectin purified from Dioclea violaceaNascimento, Ana Paula M.Knaut, Jhônatas L.Rieger, Débora K.Wolin, Ingrid A.V.Heinrich, Isabella A.Mann, JosianeJuarez, Andrea VirginiaSosa, Liliana del Vallede Paul, Ana LuciaMoreira, Cleane G.Silva, Ivanice B.Nobre, Clareane S.Osterne, Vinicius J.S.Nascimento, Kyria S.Cavada, Benildo S.Leal, Rodrigo B.AUTOPHAGYCONADIOCLEA VIOLACEADVLGLIOMALECTINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well-studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity.Fil: Nascimento, Ana Paula M.. Universidade Federal de Santa Catarina; BrasilFil: Knaut, Jhônatas L.. Universidade Federal de Santa Catarina; BrasilFil: Rieger, Débora K.. Universidade Federal de Santa Catarina; BrasilFil: Wolin, Ingrid A.V.. Universidade Federal de Santa Catarina; BrasilFil: Heinrich, Isabella A.. Universidade Federal de Santa Catarina; BrasilFil: Mann, Josiane. Universidade Federal de Santa Catarina; BrasilFil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; ArgentinaFil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; ArgentinaFil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; ArgentinaFil: Moreira, Cleane G.. Universidade Estadual do Ceará; BrasilFil: Silva, Ivanice B.. Universidade Estadual do Ceará; BrasilFil: Nobre, Clareane S.. Universidade Estadual do Ceará; BrasilFil: Osterne, Vinicius J.S.. Universidade Estadual do Ceará; BrasilFil: Nascimento, Kyria S.. Universidade Estadual do Ceará; BrasilFil: Cavada, Benildo S.. Universidade Estadual do Ceará; BrasilFil: Leal, Rodrigo B.. Universidade Federal de Santa Catarina; BrasilElsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91543Nascimento, Ana Paula M.; Knaut, Jhônatas L.; Rieger, Débora K.; Wolin, Ingrid A.V.; Heinrich, Isabella A.; et al.; Anti-glioma properties of DVL, a lectin purified from Dioclea violacea; Elsevier Science; International Journal of Biological Macromolecules; 120; 12-2018; 566-5770141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813018325443info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2018.08.106info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:01Zoai:ri.conicet.gov.ar:11336/91543instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:01.778CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
title Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
spellingShingle Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
Nascimento, Ana Paula M.
AUTOPHAGY
CONA
DIOCLEA VIOLACEA
DVL
GLIOMA
LECTIN
title_short Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
title_full Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
title_fullStr Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
title_full_unstemmed Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
title_sort Anti-glioma properties of DVL, a lectin purified from Dioclea violacea
dc.creator.none.fl_str_mv Nascimento, Ana Paula M.
Knaut, Jhônatas L.
Rieger, Débora K.
Wolin, Ingrid A.V.
Heinrich, Isabella A.
Mann, Josiane
Juarez, Andrea Virginia
Sosa, Liliana del Valle
de Paul, Ana Lucia
Moreira, Cleane G.
Silva, Ivanice B.
Nobre, Clareane S.
Osterne, Vinicius J.S.
Nascimento, Kyria S.
Cavada, Benildo S.
Leal, Rodrigo B.
author Nascimento, Ana Paula M.
author_facet Nascimento, Ana Paula M.
Knaut, Jhônatas L.
Rieger, Débora K.
Wolin, Ingrid A.V.
Heinrich, Isabella A.
Mann, Josiane
Juarez, Andrea Virginia
Sosa, Liliana del Valle
de Paul, Ana Lucia
Moreira, Cleane G.
Silva, Ivanice B.
Nobre, Clareane S.
Osterne, Vinicius J.S.
Nascimento, Kyria S.
Cavada, Benildo S.
Leal, Rodrigo B.
author_role author
author2 Knaut, Jhônatas L.
Rieger, Débora K.
Wolin, Ingrid A.V.
Heinrich, Isabella A.
Mann, Josiane
Juarez, Andrea Virginia
Sosa, Liliana del Valle
de Paul, Ana Lucia
Moreira, Cleane G.
Silva, Ivanice B.
Nobre, Clareane S.
Osterne, Vinicius J.S.
Nascimento, Kyria S.
Cavada, Benildo S.
Leal, Rodrigo B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUTOPHAGY
CONA
DIOCLEA VIOLACEA
DVL
GLIOMA
LECTIN
topic AUTOPHAGY
CONA
DIOCLEA VIOLACEA
DVL
GLIOMA
LECTIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well-studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity.
Fil: Nascimento, Ana Paula M.. Universidade Federal de Santa Catarina; Brasil
Fil: Knaut, Jhônatas L.. Universidade Federal de Santa Catarina; Brasil
Fil: Rieger, Débora K.. Universidade Federal de Santa Catarina; Brasil
Fil: Wolin, Ingrid A.V.. Universidade Federal de Santa Catarina; Brasil
Fil: Heinrich, Isabella A.. Universidade Federal de Santa Catarina; Brasil
Fil: Mann, Josiane. Universidade Federal de Santa Catarina; Brasil
Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; Argentina
Fil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; Argentina
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; Argentina
Fil: Moreira, Cleane G.. Universidade Estadual do Ceará; Brasil
Fil: Silva, Ivanice B.. Universidade Estadual do Ceará; Brasil
Fil: Nobre, Clareane S.. Universidade Estadual do Ceará; Brasil
Fil: Osterne, Vinicius J.S.. Universidade Estadual do Ceará; Brasil
Fil: Nascimento, Kyria S.. Universidade Estadual do Ceará; Brasil
Fil: Cavada, Benildo S.. Universidade Estadual do Ceará; Brasil
Fil: Leal, Rodrigo B.. Universidade Federal de Santa Catarina; Brasil
description Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well-studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91543
Nascimento, Ana Paula M.; Knaut, Jhônatas L.; Rieger, Débora K.; Wolin, Ingrid A.V.; Heinrich, Isabella A.; et al.; Anti-glioma properties of DVL, a lectin purified from Dioclea violacea; Elsevier Science; International Journal of Biological Macromolecules; 120; 12-2018; 566-577
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91543
identifier_str_mv Nascimento, Ana Paula M.; Knaut, Jhônatas L.; Rieger, Débora K.; Wolin, Ingrid A.V.; Heinrich, Isabella A.; et al.; Anti-glioma properties of DVL, a lectin purified from Dioclea violacea; Elsevier Science; International Journal of Biological Macromolecules; 120; 12-2018; 566-577
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813018325443
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2018.08.106
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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