Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles

Autores
Sánchez Campos, Sofía; Rodriguez Diez, Guadalupe; Oresti, Gerardo Martin; Salvador, Gabriela Alejandra
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metal-imbalance has been reported as a contributor factor for the degeneration of dopami-nergic neurons in Parkinson Disease (PD). Specifically, iron (Fe)-overload and copper (Cu)mis-compartmentalization have been reported to be involved in the injury of dopaminergicneurons in this pathology. The aimof this work was to characterize themechanisms ofmembrane repair by studying lipid acylation and deacylation reactions and their role in oxi-dative injury in N27 dopaminergic neurons exposed to Fe-overload and Cu-supplementa-tion. N27 dopaminergic neurons incubated with Fe (1mM) for 24 hs displayed increasedlevels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membranepermeability. Cu-supplemented neurons (10, 50μM) showed no evidence of oxidativestress markers. A different lipid acylation profile was observed in N27 neurons pre-labeledwith [3H] arachidonic acid (AA) or [3H] oleic acid (OA). In Fe-exposed neurons, AA uptakewas increased in triacylglycerols (TAG) whereas its incorporation into the phospholipid (PL)fraction was diminished. TAG content was 40% higher in Fe-exposed neurons than in con-trols. This increase was accompanied by the appearance of Nile red positive lipid bodies.Contrariwise, OA incorporation increased in the PL fractions and showed no changes inTAG. Lipid acylation profile in Cu-supplemented neurons showed AA accumulation intophosphatidylserine and no changes in TAG. The inhibition of deacylation/acylation reac-tions prompted an increase in oxidative stress markers andmitochondrial dysfunction in Fe-overloaded neurons. These findings provide evidence about the participation of lipid acyla-tionmechanisms against Fe-induced oxidative injury and postulate that dopaminergic neu-rons cleverly preserve AA in TAG in response to oxidative stress.
Fil: Sánchez Campos, Sofía. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.Bioquimicas Bahia Blanca (i); Argentina
Fil: Rodriguez Diez, Guadalupe. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.Bioquimicas Bahia Blanca (i); Argentina
Materia
DOPAMINERGIC NEURONS
LIPIDS
OXIDATIVE STRESS
ACILATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4383
Acceder
id CONICETDig_a978d10ee69f4db7ee1bc6dcc4ee378a
oai_identifier_str oai:ri.conicet.gov.ar:11336/4383
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation CyclesSánchez Campos, SofíaRodriguez Diez, GuadalupeOresti, Gerardo MartinSalvador, Gabriela AlejandraDOPAMINERGIC NEURONSLIPIDSOXIDATIVE STRESSACILATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Metal-imbalance has been reported as a contributor factor for the degeneration of dopami-nergic neurons in Parkinson Disease (PD). Specifically, iron (Fe)-overload and copper (Cu)mis-compartmentalization have been reported to be involved in the injury of dopaminergicneurons in this pathology. The aimof this work was to characterize themechanisms ofmembrane repair by studying lipid acylation and deacylation reactions and their role in oxi-dative injury in N27 dopaminergic neurons exposed to Fe-overload and Cu-supplementa-tion. N27 dopaminergic neurons incubated with Fe (1mM) for 24 hs displayed increasedlevels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membranepermeability. Cu-supplemented neurons (10, 50μM) showed no evidence of oxidativestress markers. A different lipid acylation profile was observed in N27 neurons pre-labeledwith [3H] arachidonic acid (AA) or [3H] oleic acid (OA). In Fe-exposed neurons, AA uptakewas increased in triacylglycerols (TAG) whereas its incorporation into the phospholipid (PL)fraction was diminished. TAG content was 40% higher in Fe-exposed neurons than in con-trols. This increase was accompanied by the appearance of Nile red positive lipid bodies.Contrariwise, OA incorporation increased in the PL fractions and showed no changes inTAG. Lipid acylation profile in Cu-supplemented neurons showed AA accumulation intophosphatidylserine and no changes in TAG. The inhibition of deacylation/acylation reac-tions prompted an increase in oxidative stress markers andmitochondrial dysfunction in Fe-overloaded neurons. These findings provide evidence about the participation of lipid acyla-tionmechanisms against Fe-induced oxidative injury and postulate that dopaminergic neu-rons cleverly preserve AA in TAG in response to oxidative stress.Fil: Sánchez Campos, Sofía. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.Bioquimicas Bahia Blanca (i); ArgentinaFil: Rodriguez Diez, Guadalupe. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Salvador, Gabriela Alejandra. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.Bioquimicas Bahia Blanca (i); ArgentinaPublic Library Of Science2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4383Sánchez Campos, Sofía; Rodriguez Diez, Guadalupe; Oresti, Gerardo Martin; Salvador, Gabriela Alejandra; Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles; Public Library Of Science; Plos One; 10; 6-2015; 1-201932-6203enginfo:eu-repo/semantics/altIdentifier/ark/10.1371/journal.pone.0130726info:eu-repo/semantics/altIdentifier/ark/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130726info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0130726info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:20Zoai:ri.conicet.gov.ar:11336/4383instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:21.036CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
title Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
spellingShingle Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
Sánchez Campos, Sofía
DOPAMINERGIC NEURONS
LIPIDS
OXIDATIVE STRESS
ACILATION
title_short Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
title_full Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
title_fullStr Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
title_full_unstemmed Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
title_sort Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
dc.creator.none.fl_str_mv Sánchez Campos, Sofía
Rodriguez Diez, Guadalupe
Oresti, Gerardo Martin
Salvador, Gabriela Alejandra
author Sánchez Campos, Sofía
author_facet Sánchez Campos, Sofía
Rodriguez Diez, Guadalupe
Oresti, Gerardo Martin
Salvador, Gabriela Alejandra
author_role author
author2 Rodriguez Diez, Guadalupe
Oresti, Gerardo Martin
Salvador, Gabriela Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv DOPAMINERGIC NEURONS
LIPIDS
OXIDATIVE STRESS
ACILATION
topic DOPAMINERGIC NEURONS
LIPIDS
OXIDATIVE STRESS
ACILATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Metal-imbalance has been reported as a contributor factor for the degeneration of dopami-nergic neurons in Parkinson Disease (PD). Specifically, iron (Fe)-overload and copper (Cu)mis-compartmentalization have been reported to be involved in the injury of dopaminergicneurons in this pathology. The aimof this work was to characterize themechanisms ofmembrane repair by studying lipid acylation and deacylation reactions and their role in oxi-dative injury in N27 dopaminergic neurons exposed to Fe-overload and Cu-supplementa-tion. N27 dopaminergic neurons incubated with Fe (1mM) for 24 hs displayed increasedlevels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membranepermeability. Cu-supplemented neurons (10, 50μM) showed no evidence of oxidativestress markers. A different lipid acylation profile was observed in N27 neurons pre-labeledwith [3H] arachidonic acid (AA) or [3H] oleic acid (OA). In Fe-exposed neurons, AA uptakewas increased in triacylglycerols (TAG) whereas its incorporation into the phospholipid (PL)fraction was diminished. TAG content was 40% higher in Fe-exposed neurons than in con-trols. This increase was accompanied by the appearance of Nile red positive lipid bodies.Contrariwise, OA incorporation increased in the PL fractions and showed no changes inTAG. Lipid acylation profile in Cu-supplemented neurons showed AA accumulation intophosphatidylserine and no changes in TAG. The inhibition of deacylation/acylation reac-tions prompted an increase in oxidative stress markers andmitochondrial dysfunction in Fe-overloaded neurons. These findings provide evidence about the participation of lipid acyla-tionmechanisms against Fe-induced oxidative injury and postulate that dopaminergic neu-rons cleverly preserve AA in TAG in response to oxidative stress.
Fil: Sánchez Campos, Sofía. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.Bioquimicas Bahia Blanca (i); Argentina
Fil: Rodriguez Diez, Guadalupe. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.Bioquimicas Bahia Blanca (i); Argentina
description Metal-imbalance has been reported as a contributor factor for the degeneration of dopami-nergic neurons in Parkinson Disease (PD). Specifically, iron (Fe)-overload and copper (Cu)mis-compartmentalization have been reported to be involved in the injury of dopaminergicneurons in this pathology. The aimof this work was to characterize themechanisms ofmembrane repair by studying lipid acylation and deacylation reactions and their role in oxi-dative injury in N27 dopaminergic neurons exposed to Fe-overload and Cu-supplementa-tion. N27 dopaminergic neurons incubated with Fe (1mM) for 24 hs displayed increasedlevels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membranepermeability. Cu-supplemented neurons (10, 50μM) showed no evidence of oxidativestress markers. A different lipid acylation profile was observed in N27 neurons pre-labeledwith [3H] arachidonic acid (AA) or [3H] oleic acid (OA). In Fe-exposed neurons, AA uptakewas increased in triacylglycerols (TAG) whereas its incorporation into the phospholipid (PL)fraction was diminished. TAG content was 40% higher in Fe-exposed neurons than in con-trols. This increase was accompanied by the appearance of Nile red positive lipid bodies.Contrariwise, OA incorporation increased in the PL fractions and showed no changes inTAG. Lipid acylation profile in Cu-supplemented neurons showed AA accumulation intophosphatidylserine and no changes in TAG. The inhibition of deacylation/acylation reac-tions prompted an increase in oxidative stress markers andmitochondrial dysfunction in Fe-overloaded neurons. These findings provide evidence about the participation of lipid acyla-tionmechanisms against Fe-induced oxidative injury and postulate that dopaminergic neu-rons cleverly preserve AA in TAG in response to oxidative stress.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4383
Sánchez Campos, Sofía; Rodriguez Diez, Guadalupe; Oresti, Gerardo Martin; Salvador, Gabriela Alejandra; Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles; Public Library Of Science; Plos One; 10; 6-2015; 1-20
1932-6203
url http://hdl.handle.net/11336/4383
identifier_str_mv Sánchez Campos, Sofía; Rodriguez Diez, Guadalupe; Oresti, Gerardo Martin; Salvador, Gabriela Alejandra; Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles; Public Library Of Science; Plos One; 10; 6-2015; 1-20
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/ark/10.1371/journal.pone.0130726
info:eu-repo/semantics/altIdentifier/ark/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130726
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0130726
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269399747133440
score 13.13397

Publicaciones similares