Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid

Autores
Alonso Rodríguez, N.; Martínez Lirola, M.; Chaves, F.; Iñigo, J.; Herranz, M.; Ritacco, Gloria Viviana; Bouza, E.; García de Viedma, D.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tuberculosis cases infected by the same Mycobacterium tuberculosis (MTB) strain are considered to be clustered and involved in a transmission chain. Large clusters are assumed to represent active transmission chains in a population. In the present study, we focused on the analysis of large clusters defined by IS6110-restriction fragment length polymorphism (RFLP) typing in the immigrant population in Madrid. We identified 12 large clusters (involving 43% of the isolates) comprising 4-23 representatives. We proposed a gradient of epidemiological certainty for these large clusters. For a cluster to be considered robust and a good indicator of recent transmission, the MTB strain involved should not have been identified in a geographically and epidemiologically unrelated population and the cluster had to be re-confirmed by another highly discriminative molecular marker (MIRU-VNTR). The clusters that we discovered were classified into three categories: high, intermediate and low expected epidemiological value. In the largest cluster in the study (cluster M6; 23 representatives), failures by both criteria were identified: the representative seven-band RFLP pattern was also the most prevalent in the unrelated population (25 cases) and the cluster was fully split by MIRU-15, suggesting a lack of epidemiological value. The RFLP pattern representative of this cluster was also identified in 64 isolates from five countries in the Latin American genotype database, and again proved to be heterogeneous according to the MIRU-15 analysis. Specific analysis of large clusters, combined with the application of criteria for evaluating their robustness, could help identify uninformative clusters and target epidemiological resources towards those clusters with higher expected epidemiological value.
Fil: Alonso Rodríguez, N.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); España
Fil: Martínez Lirola, M.. No especifíca;
Fil: Chaves, F.. Consejo Superior de Investigaciones Científicas; España
Fil: Iñigo, J.. No especifíca;
Fil: Herranz, M.. No especifíca;
Fil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bouza, E.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); España
Fil: García de Viedma, D.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); España
Materia
EPIDEMIOLOGY
IMMIGRANTS
LARGE CLUSTERS
PREVALENT STRAINS
TUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/186737
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/186737
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in MadridAlonso Rodríguez, N.Martínez Lirola, M.Chaves, F.Iñigo, J.Herranz, M.Ritacco, Gloria VivianaBouza, E.García de Viedma, D.EPIDEMIOLOGYIMMIGRANTSLARGE CLUSTERSPREVALENT STRAINSTUBERCULOSIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tuberculosis cases infected by the same Mycobacterium tuberculosis (MTB) strain are considered to be clustered and involved in a transmission chain. Large clusters are assumed to represent active transmission chains in a population. In the present study, we focused on the analysis of large clusters defined by IS6110-restriction fragment length polymorphism (RFLP) typing in the immigrant population in Madrid. We identified 12 large clusters (involving 43% of the isolates) comprising 4-23 representatives. We proposed a gradient of epidemiological certainty for these large clusters. For a cluster to be considered robust and a good indicator of recent transmission, the MTB strain involved should not have been identified in a geographically and epidemiologically unrelated population and the cluster had to be re-confirmed by another highly discriminative molecular marker (MIRU-VNTR). The clusters that we discovered were classified into three categories: high, intermediate and low expected epidemiological value. In the largest cluster in the study (cluster M6; 23 representatives), failures by both criteria were identified: the representative seven-band RFLP pattern was also the most prevalent in the unrelated population (25 cases) and the cluster was fully split by MIRU-15, suggesting a lack of epidemiological value. The RFLP pattern representative of this cluster was also identified in 64 isolates from five countries in the Latin American genotype database, and again proved to be heterogeneous according to the MIRU-15 analysis. Specific analysis of large clusters, combined with the application of criteria for evaluating their robustness, could help identify uninformative clusters and target epidemiological resources towards those clusters with higher expected epidemiological value.Fil: Alonso Rodríguez, N.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); EspañaFil: Martínez Lirola, M.. No especifíca;Fil: Chaves, F.. Consejo Superior de Investigaciones Científicas; EspañaFil: Iñigo, J.. No especifíca;Fil: Herranz, M.. No especifíca;Fil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bouza, E.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); EspañaFil: García de Viedma, D.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); EspañaElsevier2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/186737Alonso Rodríguez, N.; Martínez Lirola, M.; Chaves, F.; Iñigo, J.; Herranz, M.; et al.; Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid; Elsevier; Clinical Microbiology And Infection; 16; 10; 10-2010; 1544-15541198-743XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)60539-5/fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1469-0691.2010.03161.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:34Zoai:ri.conicet.gov.ar:11336/186737instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:34.994CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
title Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
spellingShingle Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
Alonso Rodríguez, N.
EPIDEMIOLOGY
IMMIGRANTS
LARGE CLUSTERS
PREVALENT STRAINS
TUBERCULOSIS
title_short Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
title_full Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
title_fullStr Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
title_full_unstemmed Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
title_sort Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid
dc.creator.none.fl_str_mv Alonso Rodríguez, N.
Martínez Lirola, M.
Chaves, F.
Iñigo, J.
Herranz, M.
Ritacco, Gloria Viviana
Bouza, E.
García de Viedma, D.
author Alonso Rodríguez, N.
author_facet Alonso Rodríguez, N.
Martínez Lirola, M.
Chaves, F.
Iñigo, J.
Herranz, M.
Ritacco, Gloria Viviana
Bouza, E.
García de Viedma, D.
author_role author
author2 Martínez Lirola, M.
Chaves, F.
Iñigo, J.
Herranz, M.
Ritacco, Gloria Viviana
Bouza, E.
García de Viedma, D.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EPIDEMIOLOGY
IMMIGRANTS
LARGE CLUSTERS
PREVALENT STRAINS
TUBERCULOSIS
topic EPIDEMIOLOGY
IMMIGRANTS
LARGE CLUSTERS
PREVALENT STRAINS
TUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tuberculosis cases infected by the same Mycobacterium tuberculosis (MTB) strain are considered to be clustered and involved in a transmission chain. Large clusters are assumed to represent active transmission chains in a population. In the present study, we focused on the analysis of large clusters defined by IS6110-restriction fragment length polymorphism (RFLP) typing in the immigrant population in Madrid. We identified 12 large clusters (involving 43% of the isolates) comprising 4-23 representatives. We proposed a gradient of epidemiological certainty for these large clusters. For a cluster to be considered robust and a good indicator of recent transmission, the MTB strain involved should not have been identified in a geographically and epidemiologically unrelated population and the cluster had to be re-confirmed by another highly discriminative molecular marker (MIRU-VNTR). The clusters that we discovered were classified into three categories: high, intermediate and low expected epidemiological value. In the largest cluster in the study (cluster M6; 23 representatives), failures by both criteria were identified: the representative seven-band RFLP pattern was also the most prevalent in the unrelated population (25 cases) and the cluster was fully split by MIRU-15, suggesting a lack of epidemiological value. The RFLP pattern representative of this cluster was also identified in 64 isolates from five countries in the Latin American genotype database, and again proved to be heterogeneous according to the MIRU-15 analysis. Specific analysis of large clusters, combined with the application of criteria for evaluating their robustness, could help identify uninformative clusters and target epidemiological resources towards those clusters with higher expected epidemiological value.
Fil: Alonso Rodríguez, N.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); España
Fil: Martínez Lirola, M.. No especifíca;
Fil: Chaves, F.. Consejo Superior de Investigaciones Científicas; España
Fil: Iñigo, J.. No especifíca;
Fil: Herranz, M.. No especifíca;
Fil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bouza, E.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); España
Fil: García de Viedma, D.. Hospital General Universitario Gregorio Marañón (hosp Gral Univ G. Marañón); España
description Tuberculosis cases infected by the same Mycobacterium tuberculosis (MTB) strain are considered to be clustered and involved in a transmission chain. Large clusters are assumed to represent active transmission chains in a population. In the present study, we focused on the analysis of large clusters defined by IS6110-restriction fragment length polymorphism (RFLP) typing in the immigrant population in Madrid. We identified 12 large clusters (involving 43% of the isolates) comprising 4-23 representatives. We proposed a gradient of epidemiological certainty for these large clusters. For a cluster to be considered robust and a good indicator of recent transmission, the MTB strain involved should not have been identified in a geographically and epidemiologically unrelated population and the cluster had to be re-confirmed by another highly discriminative molecular marker (MIRU-VNTR). The clusters that we discovered were classified into three categories: high, intermediate and low expected epidemiological value. In the largest cluster in the study (cluster M6; 23 representatives), failures by both criteria were identified: the representative seven-band RFLP pattern was also the most prevalent in the unrelated population (25 cases) and the cluster was fully split by MIRU-15, suggesting a lack of epidemiological value. The RFLP pattern representative of this cluster was also identified in 64 isolates from five countries in the Latin American genotype database, and again proved to be heterogeneous according to the MIRU-15 analysis. Specific analysis of large clusters, combined with the application of criteria for evaluating their robustness, could help identify uninformative clusters and target epidemiological resources towards those clusters with higher expected epidemiological value.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/186737
Alonso Rodríguez, N.; Martínez Lirola, M.; Chaves, F.; Iñigo, J.; Herranz, M.; et al.; Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid; Elsevier; Clinical Microbiology And Infection; 16; 10; 10-2010; 1544-1554
1198-743X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/186737
identifier_str_mv Alonso Rodríguez, N.; Martínez Lirola, M.; Chaves, F.; Iñigo, J.; Herranz, M.; et al.; Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid; Elsevier; Clinical Microbiology And Infection; 16; 10; 10-2010; 1544-1554
1198-743X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)60539-5/fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1469-0691.2010.03161.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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