Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material

Autores
Larsen, Karen Elizabeth; Perez, Vanina Andrea; Lifschitz, Adrian Luis; Virkel, Guillermo Leon
Año de publicación
2019
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Glyphosate (GLP) is one of the most commonly pesticides delivered to the environment. Farm animals could be exposed to both GLP and aminomethylphosphonic acid (AMPA, the major GLP metabolite) present in surface and groundwater, but also in foodstuffs used for preparation of concentrate feeds. For instance, detectable levels of GLP were observed in the urine of dairy cows chronically exposed to the herbicide present in their feed. In cattle, the rumen plays a central role in the pre-systemic metabolism of xenobiotics, thus protecting the organism against potentially harmful chemical compounds. This work evaluated the chemical stability of GLP in the ruminal environment. Ruminal contents from 3 steers were collected in a local abattoir. Samples were roughly filtered, kept at 37°C and transported to the laboratory. Aliquots of both whole ruminal content and fluid phase were incubated (3-6 h) in anaerobiosis with GLP (12.5 µg/mL). Metabolic viability of ruminal contents was assessed by the measurement of the SO-reduction of the anthelmintic albendazole sulfoxide (ABZSO). These assays were carried out in the absence (controls) and in presence of GLP. Incubations of boiled (inactive) ruminal content and fluid were used as controls. Samples were analysed by HLPC with fluorescence detection. Incubated ruminal contents were subsequentially centrifuged for determination of the amounts of the herbicide in the fluid phase and in the particulate material. GLP was not metabolised neither in the ruminal content nor in the fluid phase. The percentage of GLP associated to the particulate material ranged between 5.7 (3 h) and 11.2 (6 h). GLP did not affect the SO-reduction of ABZSO. These results may indicate that the ruminal environment is not involved in the pre-systemic metabolism of GLP. As GLP is a hydrophilic xenobiotic, the highest proportion of the herbicide is solubilised in the fluid phase of the ruminal content which may influence in its rate of absorption after ingestion.
Fil: Larsen, Karen Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
PESTICIDAS
CONTENIDO RUMINAL
METABOLISMO
GLIFOSATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164590
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive materialLarsen, Karen ElizabethPerez, Vanina AndreaLifschitz, Adrian LuisVirkel, Guillermo LeonPESTICIDASCONTENIDO RUMINALMETABOLISMOGLIFOSATOhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Glyphosate (GLP) is one of the most commonly pesticides delivered to the environment. Farm animals could be exposed to both GLP and aminomethylphosphonic acid (AMPA, the major GLP metabolite) present in surface and groundwater, but also in foodstuffs used for preparation of concentrate feeds. For instance, detectable levels of GLP were observed in the urine of dairy cows chronically exposed to the herbicide present in their feed. In cattle, the rumen plays a central role in the pre-systemic metabolism of xenobiotics, thus protecting the organism against potentially harmful chemical compounds. This work evaluated the chemical stability of GLP in the ruminal environment. Ruminal contents from 3 steers were collected in a local abattoir. Samples were roughly filtered, kept at 37°C and transported to the laboratory. Aliquots of both whole ruminal content and fluid phase were incubated (3-6 h) in anaerobiosis with GLP (12.5 µg/mL). Metabolic viability of ruminal contents was assessed by the measurement of the SO-reduction of the anthelmintic albendazole sulfoxide (ABZSO). These assays were carried out in the absence (controls) and in presence of GLP. Incubations of boiled (inactive) ruminal content and fluid were used as controls. Samples were analysed by HLPC with fluorescence detection. Incubated ruminal contents were subsequentially centrifuged for determination of the amounts of the herbicide in the fluid phase and in the particulate material. GLP was not metabolised neither in the ruminal content nor in the fluid phase. The percentage of GLP associated to the particulate material ranged between 5.7 (3 h) and 11.2 (6 h). GLP did not affect the SO-reduction of ABZSO. These results may indicate that the ruminal environment is not involved in the pre-systemic metabolism of GLP. As GLP is a hydrophilic xenobiotic, the highest proportion of the herbicide is solubilised in the fluid phase of the ruminal content which may influence in its rate of absorption after ingestion.Fil: Larsen, Karen Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/164590Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 286-2870025-7680CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://biologia.org.ar/downloads/Borrador_REV-MEDICINA-REUNION-2019-20200403.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:20Zoai:ri.conicet.gov.ar:11336/164590instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:21.224CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
title Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
spellingShingle Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
Larsen, Karen Elizabeth
PESTICIDAS
CONTENIDO RUMINAL
METABOLISMO
GLIFOSATO
title_short Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
title_full Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
title_fullStr Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
title_full_unstemmed Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
title_sort Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material
dc.creator.none.fl_str_mv Larsen, Karen Elizabeth
Perez, Vanina Andrea
Lifschitz, Adrian Luis
Virkel, Guillermo Leon
author Larsen, Karen Elizabeth
author_facet Larsen, Karen Elizabeth
Perez, Vanina Andrea
Lifschitz, Adrian Luis
Virkel, Guillermo Leon
author_role author
author2 Perez, Vanina Andrea
Lifschitz, Adrian Luis
Virkel, Guillermo Leon
author2_role author
author
author
dc.subject.none.fl_str_mv PESTICIDAS
CONTENIDO RUMINAL
METABOLISMO
GLIFOSATO
topic PESTICIDAS
CONTENIDO RUMINAL
METABOLISMO
GLIFOSATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Glyphosate (GLP) is one of the most commonly pesticides delivered to the environment. Farm animals could be exposed to both GLP and aminomethylphosphonic acid (AMPA, the major GLP metabolite) present in surface and groundwater, but also in foodstuffs used for preparation of concentrate feeds. For instance, detectable levels of GLP were observed in the urine of dairy cows chronically exposed to the herbicide present in their feed. In cattle, the rumen plays a central role in the pre-systemic metabolism of xenobiotics, thus protecting the organism against potentially harmful chemical compounds. This work evaluated the chemical stability of GLP in the ruminal environment. Ruminal contents from 3 steers were collected in a local abattoir. Samples were roughly filtered, kept at 37°C and transported to the laboratory. Aliquots of both whole ruminal content and fluid phase were incubated (3-6 h) in anaerobiosis with GLP (12.5 µg/mL). Metabolic viability of ruminal contents was assessed by the measurement of the SO-reduction of the anthelmintic albendazole sulfoxide (ABZSO). These assays were carried out in the absence (controls) and in presence of GLP. Incubations of boiled (inactive) ruminal content and fluid were used as controls. Samples were analysed by HLPC with fluorescence detection. Incubated ruminal contents were subsequentially centrifuged for determination of the amounts of the herbicide in the fluid phase and in the particulate material. GLP was not metabolised neither in the ruminal content nor in the fluid phase. The percentage of GLP associated to the particulate material ranged between 5.7 (3 h) and 11.2 (6 h). GLP did not affect the SO-reduction of ABZSO. These results may indicate that the ruminal environment is not involved in the pre-systemic metabolism of GLP. As GLP is a hydrophilic xenobiotic, the highest proportion of the herbicide is solubilised in the fluid phase of the ruminal content which may influence in its rate of absorption after ingestion.
Fil: Larsen, Karen Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Glyphosate (GLP) is one of the most commonly pesticides delivered to the environment. Farm animals could be exposed to both GLP and aminomethylphosphonic acid (AMPA, the major GLP metabolite) present in surface and groundwater, but also in foodstuffs used for preparation of concentrate feeds. For instance, detectable levels of GLP were observed in the urine of dairy cows chronically exposed to the herbicide present in their feed. In cattle, the rumen plays a central role in the pre-systemic metabolism of xenobiotics, thus protecting the organism against potentially harmful chemical compounds. This work evaluated the chemical stability of GLP in the ruminal environment. Ruminal contents from 3 steers were collected in a local abattoir. Samples were roughly filtered, kept at 37°C and transported to the laboratory. Aliquots of both whole ruminal content and fluid phase were incubated (3-6 h) in anaerobiosis with GLP (12.5 µg/mL). Metabolic viability of ruminal contents was assessed by the measurement of the SO-reduction of the anthelmintic albendazole sulfoxide (ABZSO). These assays were carried out in the absence (controls) and in presence of GLP. Incubations of boiled (inactive) ruminal content and fluid were used as controls. Samples were analysed by HLPC with fluorescence detection. Incubated ruminal contents were subsequentially centrifuged for determination of the amounts of the herbicide in the fluid phase and in the particulate material. GLP was not metabolised neither in the ruminal content nor in the fluid phase. The percentage of GLP associated to the particulate material ranged between 5.7 (3 h) and 11.2 (6 h). GLP did not affect the SO-reduction of ABZSO. These results may indicate that the ruminal environment is not involved in the pre-systemic metabolism of GLP. As GLP is a hydrophilic xenobiotic, the highest proportion of the herbicide is solubilised in the fluid phase of the ruminal content which may influence in its rate of absorption after ingestion.
publishDate 2019
dc.date.none.fl_str_mv 2019
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status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164590
Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 286-287
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164590
identifier_str_mv Metabolic stability of glifosate in ruminal content from cattle: partition between particulate and fluid digestive material; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 286-287
0025-7680
CONICET Digital
CONICET
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