Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy

Autores
Bazán, Paola Carolina; Micucci, Lorena; Fauro, Romina; Triquell, María Fernanda; Lo Presti, Maria Silvina; Báez, Alejandra Lidia; Strauss, Mariana; Camino, Gaston; Paglini Oliva, Patricia; Rivarola, H. Walter
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mechanisms by which patients infected with T. cruzi develop or not the disease are not completely understood. We investigated the importance of parasite persistence as a factor in generating cardiomyopathy. Fifty trypomastigote forms from the Tulahuen strain were used as inoculum for 160 Albino Swiss mice. Parasitemia, survival, histopathological and electrocardiographic studies were done on days 90, 180, 270 and 360 post infection (pi). Parasite presence in the tissues was determined by immunofluorescence. Maximum parasitemia was observed on day 21pi and became negative on day 42pi. By day 90pi, 50% of the infected mice presented electrocardiographic alterations and 67% by day 360pi; the non infected group of mice didn?t present elecrocardiographic alterations. Inflammatory infiltrates were observed throughout the infection, with a higher percentage in those mice with electrocardiographic alterations. The presence of the parasite was observed throughout the infection in skeletal muscle samples and on day 90pi in the myocardium. The highest mortality occurred on day 21pi, surviving 10% of the mice until the end of the experiments. These results demonstrate the presence of the parasite in the host tissues along the infection which could produce the injuries found in the myocardium and the electrocardiographic alterations.
Fil: Bazán, Paola Carolina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Micucci, Lorena. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Fauro, Romina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Triquell, María Fernanda. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Lo Presti, Maria Silvina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Báez, Alejandra Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Strauss, Mariana. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Camino, Gaston. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Paglini Oliva, Patricia. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Rivarola, H. Walter. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Materia
Trypanosoma cruzi
cardiomyopathy
immunofluorescence
electrocardiographic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272963
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272963
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Persistence of Trypanosoma cruzi in Experimental Chagasic CardiomyopathyBazán, Paola CarolinaMicucci, LorenaFauro, RominaTriquell, María FernandaLo Presti, Maria SilvinaBáez, Alejandra LidiaStrauss, MarianaCamino, GastonPaglini Oliva, PatriciaRivarola, H. WalterTrypanosoma cruzicardiomyopathyimmunofluorescenceelectrocardiographichttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The mechanisms by which patients infected with T. cruzi develop or not the disease are not completely understood. We investigated the importance of parasite persistence as a factor in generating cardiomyopathy. Fifty trypomastigote forms from the Tulahuen strain were used as inoculum for 160 Albino Swiss mice. Parasitemia, survival, histopathological and electrocardiographic studies were done on days 90, 180, 270 and 360 post infection (pi). Parasite presence in the tissues was determined by immunofluorescence. Maximum parasitemia was observed on day 21pi and became negative on day 42pi. By day 90pi, 50% of the infected mice presented electrocardiographic alterations and 67% by day 360pi; the non infected group of mice didn?t present elecrocardiographic alterations. Inflammatory infiltrates were observed throughout the infection, with a higher percentage in those mice with electrocardiographic alterations. The presence of the parasite was observed throughout the infection in skeletal muscle samples and on day 90pi in the myocardium. The highest mortality occurred on day 21pi, surviving 10% of the mice until the end of the experiments. These results demonstrate the presence of the parasite in the host tissues along the infection which could produce the injuries found in the myocardium and the electrocardiographic alterations.Fil: Bazán, Paola Carolina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Micucci, Lorena. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; ArgentinaFil: Fauro, Romina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Triquell, María Fernanda. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Lo Presti, Maria Silvina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Báez, Alejandra Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; ArgentinaFil: Strauss, Mariana. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Camino, Gaston. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; ArgentinaFil: Paglini Oliva, Patricia. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; ArgentinaFil: Rivarola, H. Walter. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; ArgentinaElsevier2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272963Bazán, Paola Carolina; Micucci, Lorena; Fauro, Romina; Triquell, María Fernanda; Lo Presti, Maria Silvina; et al.; Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy; Elsevier; Anti-Infective Agents; 10; 2; 3-2012; 136-1412211-3525CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.eurekaselect.com/article/41402info:eu-repo/semantics/altIdentifier/doi/10.2174/2211362611208020136info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:30Zoai:ri.conicet.gov.ar:11336/272963instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:30.562CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
title Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
spellingShingle Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
Bazán, Paola Carolina
Trypanosoma cruzi
cardiomyopathy
immunofluorescence
electrocardiographic
title_short Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
title_full Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
title_fullStr Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
title_full_unstemmed Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
title_sort Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
dc.creator.none.fl_str_mv Bazán, Paola Carolina
Micucci, Lorena
Fauro, Romina
Triquell, María Fernanda
Lo Presti, Maria Silvina
Báez, Alejandra Lidia
Strauss, Mariana
Camino, Gaston
Paglini Oliva, Patricia
Rivarola, H. Walter
author Bazán, Paola Carolina
author_facet Bazán, Paola Carolina
Micucci, Lorena
Fauro, Romina
Triquell, María Fernanda
Lo Presti, Maria Silvina
Báez, Alejandra Lidia
Strauss, Mariana
Camino, Gaston
Paglini Oliva, Patricia
Rivarola, H. Walter
author_role author
author2 Micucci, Lorena
Fauro, Romina
Triquell, María Fernanda
Lo Presti, Maria Silvina
Báez, Alejandra Lidia
Strauss, Mariana
Camino, Gaston
Paglini Oliva, Patricia
Rivarola, H. Walter
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma cruzi
cardiomyopathy
immunofluorescence
electrocardiographic
topic Trypanosoma cruzi
cardiomyopathy
immunofluorescence
electrocardiographic
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The mechanisms by which patients infected with T. cruzi develop or not the disease are not completely understood. We investigated the importance of parasite persistence as a factor in generating cardiomyopathy. Fifty trypomastigote forms from the Tulahuen strain were used as inoculum for 160 Albino Swiss mice. Parasitemia, survival, histopathological and electrocardiographic studies were done on days 90, 180, 270 and 360 post infection (pi). Parasite presence in the tissues was determined by immunofluorescence. Maximum parasitemia was observed on day 21pi and became negative on day 42pi. By day 90pi, 50% of the infected mice presented electrocardiographic alterations and 67% by day 360pi; the non infected group of mice didn?t present elecrocardiographic alterations. Inflammatory infiltrates were observed throughout the infection, with a higher percentage in those mice with electrocardiographic alterations. The presence of the parasite was observed throughout the infection in skeletal muscle samples and on day 90pi in the myocardium. The highest mortality occurred on day 21pi, surviving 10% of the mice until the end of the experiments. These results demonstrate the presence of the parasite in the host tissues along the infection which could produce the injuries found in the myocardium and the electrocardiographic alterations.
Fil: Bazán, Paola Carolina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Micucci, Lorena. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Fauro, Romina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Triquell, María Fernanda. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Lo Presti, Maria Silvina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Báez, Alejandra Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Strauss, Mariana. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Camino, Gaston. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Paglini Oliva, Patricia. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
Fil: Rivarola, H. Walter. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica; Argentina
description The mechanisms by which patients infected with T. cruzi develop or not the disease are not completely understood. We investigated the importance of parasite persistence as a factor in generating cardiomyopathy. Fifty trypomastigote forms from the Tulahuen strain were used as inoculum for 160 Albino Swiss mice. Parasitemia, survival, histopathological and electrocardiographic studies were done on days 90, 180, 270 and 360 post infection (pi). Parasite presence in the tissues was determined by immunofluorescence. Maximum parasitemia was observed on day 21pi and became negative on day 42pi. By day 90pi, 50% of the infected mice presented electrocardiographic alterations and 67% by day 360pi; the non infected group of mice didn?t present elecrocardiographic alterations. Inflammatory infiltrates were observed throughout the infection, with a higher percentage in those mice with electrocardiographic alterations. The presence of the parasite was observed throughout the infection in skeletal muscle samples and on day 90pi in the myocardium. The highest mortality occurred on day 21pi, surviving 10% of the mice until the end of the experiments. These results demonstrate the presence of the parasite in the host tissues along the infection which could produce the injuries found in the myocardium and the electrocardiographic alterations.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272963
Bazán, Paola Carolina; Micucci, Lorena; Fauro, Romina; Triquell, María Fernanda; Lo Presti, Maria Silvina; et al.; Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy; Elsevier; Anti-Infective Agents; 10; 2; 3-2012; 136-141
2211-3525
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272963
identifier_str_mv Bazán, Paola Carolina; Micucci, Lorena; Fauro, Romina; Triquell, María Fernanda; Lo Presti, Maria Silvina; et al.; Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy; Elsevier; Anti-Infective Agents; 10; 2; 3-2012; 136-141
2211-3525
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.eurekaselect.com/article/41402
info:eu-repo/semantics/altIdentifier/doi/10.2174/2211362611208020136
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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