Phase state and surface topography of palmitoyl-ceramide monolayers

Autores
Fanani, Maria Laura; Maggio, Bruno
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In cell biology (and in many biophysical) studies there is a natural tendency to consider ceramide as a highly condensed, solid-type lipid conferring rigidity and close packing to biomembranes. In the present work we advanced the understanding of the phase behavior of palmitoyl-ceramide restricted to a planar interface using Langmuir monolayers under strictly controlled and known surface packing conditions. Surface pressure-molecular area isotherms were complemented with molecular area-temperature isobars and with observations of the surface topography by Brewster Angle Microscopy. The results described herein indicate that palmitoyl-ceramide can exhibit expanded, as well as condensed phase states. Formation of three phases was found, depending on the surface pressure and temperature: a solid (1.80nm thick), a liquid-condensed (1.73nm thick, likely tilted) and a liquid-expanded (1.54nm thick) phase over the temperature range 5-62 °C. A large hysteretic behavior is observed for the S phase monolayer that may indicate high resistance to domain boundary deformation. A second (or higher) order S→LC phase transition is observed at about room temperature while a first order LC→LE transition occurs in a range of temperature encompassing the physiological one (observed above 30 °C at low surface pressure). This phase behavior broadens the view of ceramide as a type of lipid not-always-rigid but able to exhibit polymorphic properties.
Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
BREWSTER ANGLE MICROSCOPY
ISOBARS
LIPID DOMAINS
MONOLAYER THICKNESS
PHASE DIAGRAM
SPHINGOLIPIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/186350
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phase state and surface topography of palmitoyl-ceramide monolayersFanani, Maria LauraMaggio, BrunoBREWSTER ANGLE MICROSCOPYISOBARSLIPID DOMAINSMONOLAYER THICKNESSPHASE DIAGRAMSPHINGOLIPIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In cell biology (and in many biophysical) studies there is a natural tendency to consider ceramide as a highly condensed, solid-type lipid conferring rigidity and close packing to biomembranes. In the present work we advanced the understanding of the phase behavior of palmitoyl-ceramide restricted to a planar interface using Langmuir monolayers under strictly controlled and known surface packing conditions. Surface pressure-molecular area isotherms were complemented with molecular area-temperature isobars and with observations of the surface topography by Brewster Angle Microscopy. The results described herein indicate that palmitoyl-ceramide can exhibit expanded, as well as condensed phase states. Formation of three phases was found, depending on the surface pressure and temperature: a solid (1.80nm thick), a liquid-condensed (1.73nm thick, likely tilted) and a liquid-expanded (1.54nm thick) phase over the temperature range 5-62 °C. A large hysteretic behavior is observed for the S phase monolayer that may indicate high resistance to domain boundary deformation. A second (or higher) order S→LC phase transition is observed at about room temperature while a first order LC→LE transition occurs in a range of temperature encompassing the physiological one (observed above 30 °C at low surface pressure). This phase behavior broadens the view of ceramide as a type of lipid not-always-rigid but able to exhibit polymorphic properties.Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaElsevier Ireland2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/186350Fanani, Maria Laura; Maggio, Bruno; Phase state and surface topography of palmitoyl-ceramide monolayers; Elsevier Ireland; Chemistry and Physics of Lipids; 163; 6; 6-2010; 594-6000009-30841873-2941CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308410000745info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2010.04.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:49:47Zoai:ri.conicet.gov.ar:11336/186350instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:49:48.13CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phase state and surface topography of palmitoyl-ceramide monolayers
title Phase state and surface topography of palmitoyl-ceramide monolayers
spellingShingle Phase state and surface topography of palmitoyl-ceramide monolayers
Fanani, Maria Laura
BREWSTER ANGLE MICROSCOPY
ISOBARS
LIPID DOMAINS
MONOLAYER THICKNESS
PHASE DIAGRAM
SPHINGOLIPIDS
title_short Phase state and surface topography of palmitoyl-ceramide monolayers
title_full Phase state and surface topography of palmitoyl-ceramide monolayers
title_fullStr Phase state and surface topography of palmitoyl-ceramide monolayers
title_full_unstemmed Phase state and surface topography of palmitoyl-ceramide monolayers
title_sort Phase state and surface topography of palmitoyl-ceramide monolayers
dc.creator.none.fl_str_mv Fanani, Maria Laura
Maggio, Bruno
author Fanani, Maria Laura
author_facet Fanani, Maria Laura
Maggio, Bruno
author_role author
author2 Maggio, Bruno
author2_role author
dc.subject.none.fl_str_mv BREWSTER ANGLE MICROSCOPY
ISOBARS
LIPID DOMAINS
MONOLAYER THICKNESS
PHASE DIAGRAM
SPHINGOLIPIDS
topic BREWSTER ANGLE MICROSCOPY
ISOBARS
LIPID DOMAINS
MONOLAYER THICKNESS
PHASE DIAGRAM
SPHINGOLIPIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In cell biology (and in many biophysical) studies there is a natural tendency to consider ceramide as a highly condensed, solid-type lipid conferring rigidity and close packing to biomembranes. In the present work we advanced the understanding of the phase behavior of palmitoyl-ceramide restricted to a planar interface using Langmuir monolayers under strictly controlled and known surface packing conditions. Surface pressure-molecular area isotherms were complemented with molecular area-temperature isobars and with observations of the surface topography by Brewster Angle Microscopy. The results described herein indicate that palmitoyl-ceramide can exhibit expanded, as well as condensed phase states. Formation of three phases was found, depending on the surface pressure and temperature: a solid (1.80nm thick), a liquid-condensed (1.73nm thick, likely tilted) and a liquid-expanded (1.54nm thick) phase over the temperature range 5-62 °C. A large hysteretic behavior is observed for the S phase monolayer that may indicate high resistance to domain boundary deformation. A second (or higher) order S→LC phase transition is observed at about room temperature while a first order LC→LE transition occurs in a range of temperature encompassing the physiological one (observed above 30 °C at low surface pressure). This phase behavior broadens the view of ceramide as a type of lipid not-always-rigid but able to exhibit polymorphic properties.
Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description In cell biology (and in many biophysical) studies there is a natural tendency to consider ceramide as a highly condensed, solid-type lipid conferring rigidity and close packing to biomembranes. In the present work we advanced the understanding of the phase behavior of palmitoyl-ceramide restricted to a planar interface using Langmuir monolayers under strictly controlled and known surface packing conditions. Surface pressure-molecular area isotherms were complemented with molecular area-temperature isobars and with observations of the surface topography by Brewster Angle Microscopy. The results described herein indicate that palmitoyl-ceramide can exhibit expanded, as well as condensed phase states. Formation of three phases was found, depending on the surface pressure and temperature: a solid (1.80nm thick), a liquid-condensed (1.73nm thick, likely tilted) and a liquid-expanded (1.54nm thick) phase over the temperature range 5-62 °C. A large hysteretic behavior is observed for the S phase monolayer that may indicate high resistance to domain boundary deformation. A second (or higher) order S→LC phase transition is observed at about room temperature while a first order LC→LE transition occurs in a range of temperature encompassing the physiological one (observed above 30 °C at low surface pressure). This phase behavior broadens the view of ceramide as a type of lipid not-always-rigid but able to exhibit polymorphic properties.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/186350
Fanani, Maria Laura; Maggio, Bruno; Phase state and surface topography of palmitoyl-ceramide monolayers; Elsevier Ireland; Chemistry and Physics of Lipids; 163; 6; 6-2010; 594-600
0009-3084
1873-2941
CONICET Digital
CONICET
url http://hdl.handle.net/11336/186350
identifier_str_mv Fanani, Maria Laura; Maggio, Bruno; Phase state and surface topography of palmitoyl-ceramide monolayers; Elsevier Ireland; Chemistry and Physics of Lipids; 163; 6; 6-2010; 594-600
0009-3084
1873-2941
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308410000745
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2010.04.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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