Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis

Autores
Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; Balaszczuk, Ana Maria; Fellet, Andrea L.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.
Fil: Sarati, Lorena Ivonne. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Martinez, Carla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Uceda, Ana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Balaszczuk, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia
AGED
RENAL FUNCTION
THYROID HORMONES
WATER CHANNEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84987

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasisSarati, Lorena IvonneToblli, Jorge EduardoMartinez, Carla RominaUceda, Ana MargaritaFeldman, Mariana LauraBalaszczuk, Ana MariaFellet, Andrea L.AGEDRENAL FUNCTIONTHYROID HORMONESWATER CHANNELhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.Fil: Sarati, Lorena Ivonne. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; ArgentinaFil: Martinez, Carla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Uceda, Ana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; ArgentinaFil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; ArgentinaFil: Balaszczuk, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaW B Saunders Co-Elsevier Inc2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84987Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; et al.; Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis; W B Saunders Co-Elsevier Inc; Metabolism-clinical And Experimental; 62; 9; 9-2013; 1287-12950026-0495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026049513001303info:eu-repo/semantics/altIdentifier/doi/10.1016/j.metabol.2013.04.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:42Zoai:ri.conicet.gov.ar:11336/84987instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:43.233CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
title Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
spellingShingle Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
Sarati, Lorena Ivonne
AGED
RENAL FUNCTION
THYROID HORMONES
WATER CHANNEL
title_short Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
title_full Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
title_fullStr Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
title_full_unstemmed Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
title_sort Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
dc.creator.none.fl_str_mv Sarati, Lorena Ivonne
Toblli, Jorge Eduardo
Martinez, Carla Romina
Uceda, Ana Margarita
Feldman, Mariana Laura
Balaszczuk, Ana Maria
Fellet, Andrea L.
author Sarati, Lorena Ivonne
author_facet Sarati, Lorena Ivonne
Toblli, Jorge Eduardo
Martinez, Carla Romina
Uceda, Ana Margarita
Feldman, Mariana Laura
Balaszczuk, Ana Maria
Fellet, Andrea L.
author_role author
author2 Toblli, Jorge Eduardo
Martinez, Carla Romina
Uceda, Ana Margarita
Feldman, Mariana Laura
Balaszczuk, Ana Maria
Fellet, Andrea L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AGED
RENAL FUNCTION
THYROID HORMONES
WATER CHANNEL
topic AGED
RENAL FUNCTION
THYROID HORMONES
WATER CHANNEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.
Fil: Sarati, Lorena Ivonne. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Martinez, Carla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Uceda, Ana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Balaszczuk, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84987
Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; et al.; Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis; W B Saunders Co-Elsevier Inc; Metabolism-clinical And Experimental; 62; 9; 9-2013; 1287-1295
0026-0495
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84987
identifier_str_mv Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; et al.; Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis; W B Saunders Co-Elsevier Inc; Metabolism-clinical And Experimental; 62; 9; 9-2013; 1287-1295
0026-0495
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026049513001303
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.metabol.2013.04.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv W B Saunders Co-Elsevier Inc
publisher.none.fl_str_mv W B Saunders Co-Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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