Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis
- Autores
- Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; Balaszczuk, Ana Maria; Fellet, Andrea L.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.
Fil: Sarati, Lorena Ivonne. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Martinez, Carla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Uceda, Ana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina
Fil: Balaszczuk, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina - Materia
-
AGED
RENAL FUNCTION
THYROID HORMONES
WATER CHANNEL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/84987
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Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasisSarati, Lorena IvonneToblli, Jorge EduardoMartinez, Carla RominaUceda, Ana MargaritaFeldman, Mariana LauraBalaszczuk, Ana MariaFellet, Andrea L.AGEDRENAL FUNCTIONTHYROID HORMONESWATER CHANNELhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis.Fil: Sarati, Lorena Ivonne. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; ArgentinaFil: Martinez, Carla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Uceda, Ana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; ArgentinaFil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; ArgentinaFil: Balaszczuk, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaW B Saunders Co-Elsevier Inc2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84987Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; et al.; Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis; W B Saunders Co-Elsevier Inc; Metabolism-clinical And Experimental; 62; 9; 9-2013; 1287-12950026-0495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026049513001303info:eu-repo/semantics/altIdentifier/doi/10.1016/j.metabol.2013.04.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:42Zoai:ri.conicet.gov.ar:11336/84987instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:43.233CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
title |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
spellingShingle |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis Sarati, Lorena Ivonne AGED RENAL FUNCTION THYROID HORMONES WATER CHANNEL |
title_short |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
title_full |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
title_fullStr |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
title_full_unstemmed |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
title_sort |
Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis |
dc.creator.none.fl_str_mv |
Sarati, Lorena Ivonne Toblli, Jorge Eduardo Martinez, Carla Romina Uceda, Ana Margarita Feldman, Mariana Laura Balaszczuk, Ana Maria Fellet, Andrea L. |
author |
Sarati, Lorena Ivonne |
author_facet |
Sarati, Lorena Ivonne Toblli, Jorge Eduardo Martinez, Carla Romina Uceda, Ana Margarita Feldman, Mariana Laura Balaszczuk, Ana Maria Fellet, Andrea L. |
author_role |
author |
author2 |
Toblli, Jorge Eduardo Martinez, Carla Romina Uceda, Ana Margarita Feldman, Mariana Laura Balaszczuk, Ana Maria Fellet, Andrea L. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
AGED RENAL FUNCTION THYROID HORMONES WATER CHANNEL |
topic |
AGED RENAL FUNCTION THYROID HORMONES WATER CHANNEL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis. Fil: Sarati, Lorena Ivonne. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina Fil: Martinez, Carla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Uceda, Ana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina Fil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman. Laboratorio de Medicina Experimental; Argentina Fil: Balaszczuk, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina |
description |
Objective Hypothyroid state and aging are associated with impairment in water reabsorption and changes in aquaporin water channel type 2 (AQP2). Nitric oxide (NO) is involved in AQP2 trafficking to the apical plasma membrane in medullary collecting duct cells. The purpose of this study was to investigate whether aging and hypothyroidism alter renal function, and whether medullary NO and AQP2 are implicated in maintaining water homeostasis. Materials/Methods Sprague-Dawley rats aged 2 and 18 months old were treated with 0.02% methimazole (w/v) during 28 days. Renal function was examined and NO synthase (NOS) activity ([14C (U)]-L-arginine to [14C (U)]-L-citrulline assays), NOS, caveolin-1 and -3 and AQP2 protein levels were determined in medullary tissue (Western blot). Plasma membrane fraction and intracellular vesicle fraction of AQP2 were evaluated by Western blot and immunohistochemistry. Results A divergent response was observed in hypothyroid rats: while young rats exhibited polyuria with decreased medullary NOS activity, adult rats exhibited a decrease in urine output with increased NOS activity. AQP2 was increased with hypothyroidism, but while young rats exhibited increased AQP2 in plasma membrane, adult rats did so in the cytosolic site. Conclusions Hypothyroidism contributes in a differential way to aging-induced changes in renal function, and medullary NO and AQP2 would be implicated in maintaining water homeostasis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/84987 Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; et al.; Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis; W B Saunders Co-Elsevier Inc; Metabolism-clinical And Experimental; 62; 9; 9-2013; 1287-1295 0026-0495 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/84987 |
identifier_str_mv |
Sarati, Lorena Ivonne; Toblli, Jorge Eduardo; Martinez, Carla Romina; Uceda, Ana Margarita; Feldman, Mariana Laura; et al.; Nitric oxide and AQP2 in hypothyroid rats: A link between aging and water homeostasis; W B Saunders Co-Elsevier Inc; Metabolism-clinical And Experimental; 62; 9; 9-2013; 1287-1295 0026-0495 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026049513001303 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.metabol.2013.04.013 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
W B Saunders Co-Elsevier Inc |
publisher.none.fl_str_mv |
W B Saunders Co-Elsevier Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614304400146432 |
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13.070432 |