Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions

Autores
Santos, Felipe; Vera, José Luis; Lamosa, Pedro; Font, Graciela Maria; de Vos, Willem M.; Santos, Helena; Sesma, Fernando Juan Manuel; Hugenholtz, Jeroen
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coα-[α-(7-adenyl)]-Coβ-cyanocobamide or pseudovitamin B12. This molecule differs from cobalamin in the α-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a α-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12. © 2007 Federation of European Biochemical Societies.
Fil: Santos, Felipe. NIZO Food Research; Países Bajos
Fil: Vera, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Lamosa, Pedro. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; Portugal
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Vos, Willem M.. Wageningen University and Research Centre.Laboratory of Microbiology ; Países Bajos
Fil: Santos, Helena. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; Portugal
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hugenholtz, Jeroen. NIZO Food Research; Países Bajos
Materia
B12 Analogues
Cobamide
Corrinoids
Lactic Acid Bacteria
Lactobacillus Reuteri
Pseudovitamin B12
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58580
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/58580
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditionsSantos, FelipeVera, José LuisLamosa, PedroFont, Graciela Mariade Vos, Willem M.Santos, HelenaSesma, Fernando Juan ManuelHugenholtz, JeroenB12 AnaloguesCobamideCorrinoidsLactic Acid BacteriaLactobacillus ReuteriPseudovitamin B12https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coα-[α-(7-adenyl)]-Coβ-cyanocobamide or pseudovitamin B12. This molecule differs from cobalamin in the α-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a α-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12. © 2007 Federation of European Biochemical Societies.Fil: Santos, Felipe. NIZO Food Research; Países BajosFil: Vera, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Lamosa, Pedro. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; PortugalFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Vos, Willem M.. Wageningen University and Research Centre.Laboratory of Microbiology ; Países BajosFil: Santos, Helena. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; PortugalFil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Hugenholtz, Jeroen. NIZO Food Research; Países BajosFEBS Press2007-10-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58580Santos, Felipe; Vera, José Luis; Lamosa, Pedro; Font, Graciela Maria; de Vos, Willem M.; et al.; Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions; FEBS Press; FEBS Letters; 581; 25; 17-10-2007; 4865-48700014-57931873-3468CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2007.09.012info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2007.09.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:13Zoai:ri.conicet.gov.ar:11336/58580instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:13.46CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
title Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
spellingShingle Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
Santos, Felipe
B12 Analogues
Cobamide
Corrinoids
Lactic Acid Bacteria
Lactobacillus Reuteri
Pseudovitamin B12
title_short Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
title_full Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
title_fullStr Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
title_full_unstemmed Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
title_sort Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
dc.creator.none.fl_str_mv Santos, Felipe
Vera, José Luis
Lamosa, Pedro
Font, Graciela Maria
de Vos, Willem M.
Santos, Helena
Sesma, Fernando Juan Manuel
Hugenholtz, Jeroen
author Santos, Felipe
author_facet Santos, Felipe
Vera, José Luis
Lamosa, Pedro
Font, Graciela Maria
de Vos, Willem M.
Santos, Helena
Sesma, Fernando Juan Manuel
Hugenholtz, Jeroen
author_role author
author2 Vera, José Luis
Lamosa, Pedro
Font, Graciela Maria
de Vos, Willem M.
Santos, Helena
Sesma, Fernando Juan Manuel
Hugenholtz, Jeroen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv B12 Analogues
Cobamide
Corrinoids
Lactic Acid Bacteria
Lactobacillus Reuteri
Pseudovitamin B12
topic B12 Analogues
Cobamide
Corrinoids
Lactic Acid Bacteria
Lactobacillus Reuteri
Pseudovitamin B12
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coα-[α-(7-adenyl)]-Coβ-cyanocobamide or pseudovitamin B12. This molecule differs from cobalamin in the α-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a α-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12. © 2007 Federation of European Biochemical Societies.
Fil: Santos, Felipe. NIZO Food Research; Países Bajos
Fil: Vera, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Lamosa, Pedro. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; Portugal
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Vos, Willem M.. Wageningen University and Research Centre.Laboratory of Microbiology ; Países Bajos
Fil: Santos, Helena. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; Portugal
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hugenholtz, Jeroen. NIZO Food Research; Países Bajos
description We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coα-[α-(7-adenyl)]-Coβ-cyanocobamide or pseudovitamin B12. This molecule differs from cobalamin in the α-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a α-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12. © 2007 Federation of European Biochemical Societies.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58580
Santos, Felipe; Vera, José Luis; Lamosa, Pedro; Font, Graciela Maria; de Vos, Willem M.; et al.; Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions; FEBS Press; FEBS Letters; 581; 25; 17-10-2007; 4865-4870
0014-5793
1873-3468
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58580
identifier_str_mv Santos, Felipe; Vera, José Luis; Lamosa, Pedro; Font, Graciela Maria; de Vos, Willem M.; et al.; Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions; FEBS Press; FEBS Letters; 581; 25; 17-10-2007; 4865-4870
0014-5793
1873-3468
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2007.09.012
info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2007.09.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv FEBS Press
publisher.none.fl_str_mv FEBS Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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