A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120

Autores
Valladares, Ana; Rodríguez, Virginia; Camargo, Sergio; Martínez Noël, Giselle María Astrid; Herrero, Antonia; Luque, Ignacio
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative −35 and −10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase activity of the mutant was low under oxic conditions, likely resulting from inefficient protection against oxygen. In line with this, the activation of the coxB2A2C2 and coxB3A3C3 operons, encoding heterocyst-specific terminal respiratory oxidases responsible for internal oxygen removal, was deficient in the pipX mutant. Therefore, the Anabaena PipX factor shows a spatiotemporal specificity contributing to normal heterocyst function, including full activation of the nitrogenase structural genes and genes of the nitrogenase-protective features of the heterocyst.
Fil: Valladares, Ana. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Rodríguez, Virginia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Camargo, Sergio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Martínez Noël, Giselle María Astrid. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Herrero, Antonia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Luque, Ignacio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Materia
Pipx
Anabaena
Heterocyst Function
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15707
Acceder
id CONICETDig_a734986c13c8383cc7190e2a8073e990
oai_identifier_str oai:ri.conicet.gov.ar:11336/15707
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120Valladares, AnaRodríguez, VirginiaCamargo, SergioMartínez Noël, Giselle María AstridHerrero, AntoniaLuque, IgnacioPipxAnabaenaHeterocyst Functionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative −35 and −10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase activity of the mutant was low under oxic conditions, likely resulting from inefficient protection against oxygen. In line with this, the activation of the coxB2A2C2 and coxB3A3C3 operons, encoding heterocyst-specific terminal respiratory oxidases responsible for internal oxygen removal, was deficient in the pipX mutant. Therefore, the Anabaena PipX factor shows a spatiotemporal specificity contributing to normal heterocyst function, including full activation of the nitrogenase structural genes and genes of the nitrogenase-protective features of the heterocyst.Fil: Valladares, Ana. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; EspañaFil: Rodríguez, Virginia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; EspañaFil: Camargo, Sergio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; EspañaFil: Martínez Noël, Giselle María Astrid. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Herrero, Antonia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; EspañaFil: Luque, Ignacio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; EspañaAmerican Society For Microbiology2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15707Valladares, Ana; Rodríguez, Virginia; Camargo, Sergio; Martínez Noël, Giselle María Astrid; Herrero, Antonia; et al.; A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120; American Society For Microbiology; Journal Of Bacteriology; 193; 5; 3-2011; 1172-11820021-9193enginfo:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/5/1172.shortinfo:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01202-10info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:19Zoai:ri.conicet.gov.ar:11336/15707instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:19.295CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
title A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
spellingShingle A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
Valladares, Ana
Pipx
Anabaena
Heterocyst Function
title_short A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
title_full A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
title_fullStr A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
title_full_unstemmed A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
title_sort A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120
dc.creator.none.fl_str_mv Valladares, Ana
Rodríguez, Virginia
Camargo, Sergio
Martínez Noël, Giselle María Astrid
Herrero, Antonia
Luque, Ignacio
author Valladares, Ana
author_facet Valladares, Ana
Rodríguez, Virginia
Camargo, Sergio
Martínez Noël, Giselle María Astrid
Herrero, Antonia
Luque, Ignacio
author_role author
author2 Rodríguez, Virginia
Camargo, Sergio
Martínez Noël, Giselle María Astrid
Herrero, Antonia
Luque, Ignacio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pipx
Anabaena
Heterocyst Function
topic Pipx
Anabaena
Heterocyst Function
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative −35 and −10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase activity of the mutant was low under oxic conditions, likely resulting from inefficient protection against oxygen. In line with this, the activation of the coxB2A2C2 and coxB3A3C3 operons, encoding heterocyst-specific terminal respiratory oxidases responsible for internal oxygen removal, was deficient in the pipX mutant. Therefore, the Anabaena PipX factor shows a spatiotemporal specificity contributing to normal heterocyst function, including full activation of the nitrogenase structural genes and genes of the nitrogenase-protective features of the heterocyst.
Fil: Valladares, Ana. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Rodríguez, Virginia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Camargo, Sergio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Martínez Noël, Giselle María Astrid. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Herrero, Antonia. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
Fil: Luque, Ignacio. Consejo Superior de Investigaciones Cientificas; España. Universidad de Sevilla; España
description The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative −35 and −10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase activity of the mutant was low under oxic conditions, likely resulting from inefficient protection against oxygen. In line with this, the activation of the coxB2A2C2 and coxB3A3C3 operons, encoding heterocyst-specific terminal respiratory oxidases responsible for internal oxygen removal, was deficient in the pipX mutant. Therefore, the Anabaena PipX factor shows a spatiotemporal specificity contributing to normal heterocyst function, including full activation of the nitrogenase structural genes and genes of the nitrogenase-protective features of the heterocyst.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15707
Valladares, Ana; Rodríguez, Virginia; Camargo, Sergio; Martínez Noël, Giselle María Astrid; Herrero, Antonia; et al.; A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120; American Society For Microbiology; Journal Of Bacteriology; 193; 5; 3-2011; 1172-1182
0021-9193
url http://hdl.handle.net/11336/15707
identifier_str_mv Valladares, Ana; Rodríguez, Virginia; Camargo, Sergio; Martínez Noël, Giselle María Astrid; Herrero, Antonia; et al.; A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120; American Society For Microbiology; Journal Of Bacteriology; 193; 5; 3-2011; 1172-1182
0021-9193
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/5/1172.short
info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01202-10
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981347699720192
score 12.48226

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