Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp

Autores
Curatti, Leonardo; Flores, Enrique; Salerno, Graciela Lidia
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sucrose synthase (SuS) expression was studied in the filamentous, nitrogen-fixing cyanobacterium Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels were lower in cells cultured diazotrophically than in the presence of combined nitrogen. An insertional susA mutant presented a dramatic increase in sucrose levels, whereas the disaccharide was not detectable in a susA overexpressing strain, indicating that SusA is involved in the cleavage of sucrose in vivo. Diazotrophic growth was impaired in the susA overexpressing strain, suggesting a role for sucrose in diazotrophic metabolism and the involvement of SusA in the control of carbon flux in the N2-fixing filament.
Fil: Curatti, Leonardo. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Flores, Enrique. Universidad de Sevilla; España
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANABAENA SP. PCC 7119
CYANOBACTERIA
NITROGEN METABOLISM
SUCROSE METABOLISM
SUCROSE SYNTHASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150301
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/150301
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena spCuratti, LeonardoFlores, EnriqueSalerno, Graciela LidiaANABAENA SP. PCC 7119CYANOBACTERIANITROGEN METABOLISMSUCROSE METABOLISMSUCROSE SYNTHASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Sucrose synthase (SuS) expression was studied in the filamentous, nitrogen-fixing cyanobacterium Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels were lower in cells cultured diazotrophically than in the presence of combined nitrogen. An insertional susA mutant presented a dramatic increase in sucrose levels, whereas the disaccharide was not detectable in a susA overexpressing strain, indicating that SusA is involved in the cleavage of sucrose in vivo. Diazotrophic growth was impaired in the susA overexpressing strain, suggesting a role for sucrose in diazotrophic metabolism and the involvement of SusA in the control of carbon flux in the N2-fixing filament.Fil: Curatti, Leonardo. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Flores, Enrique. Universidad de Sevilla; EspañaFil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2002-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150301Curatti, Leonardo; Flores, Enrique; Salerno, Graciela Lidia; Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp; Elsevier Science; FEBS Letters; 513; 2-3; 2-2002; 175-1780014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/S0014-5793%2802%2902283-4?sid=nlm%3Apubmedinfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0014-5793(02)02283-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:23:54Zoai:ri.conicet.gov.ar:11336/150301instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:23:54.656CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
title Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
spellingShingle Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
Curatti, Leonardo
ANABAENA SP. PCC 7119
CYANOBACTERIA
NITROGEN METABOLISM
SUCROSE METABOLISM
SUCROSE SYNTHASE
title_short Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
title_full Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
title_fullStr Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
title_full_unstemmed Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
title_sort Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
dc.creator.none.fl_str_mv Curatti, Leonardo
Flores, Enrique
Salerno, Graciela Lidia
author Curatti, Leonardo
author_facet Curatti, Leonardo
Flores, Enrique
Salerno, Graciela Lidia
author_role author
author2 Flores, Enrique
Salerno, Graciela Lidia
author2_role author
author
dc.subject.none.fl_str_mv ANABAENA SP. PCC 7119
CYANOBACTERIA
NITROGEN METABOLISM
SUCROSE METABOLISM
SUCROSE SYNTHASE
topic ANABAENA SP. PCC 7119
CYANOBACTERIA
NITROGEN METABOLISM
SUCROSE METABOLISM
SUCROSE SYNTHASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sucrose synthase (SuS) expression was studied in the filamentous, nitrogen-fixing cyanobacterium Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels were lower in cells cultured diazotrophically than in the presence of combined nitrogen. An insertional susA mutant presented a dramatic increase in sucrose levels, whereas the disaccharide was not detectable in a susA overexpressing strain, indicating that SusA is involved in the cleavage of sucrose in vivo. Diazotrophic growth was impaired in the susA overexpressing strain, suggesting a role for sucrose in diazotrophic metabolism and the involvement of SusA in the control of carbon flux in the N2-fixing filament.
Fil: Curatti, Leonardo. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Flores, Enrique. Universidad de Sevilla; España
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Sucrose synthase (SuS) expression was studied in the filamentous, nitrogen-fixing cyanobacterium Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels were lower in cells cultured diazotrophically than in the presence of combined nitrogen. An insertional susA mutant presented a dramatic increase in sucrose levels, whereas the disaccharide was not detectable in a susA overexpressing strain, indicating that SusA is involved in the cleavage of sucrose in vivo. Diazotrophic growth was impaired in the susA overexpressing strain, suggesting a role for sucrose in diazotrophic metabolism and the involvement of SusA in the control of carbon flux in the N2-fixing filament.
publishDate 2002
dc.date.none.fl_str_mv 2002-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150301
Curatti, Leonardo; Flores, Enrique; Salerno, Graciela Lidia; Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp; Elsevier Science; FEBS Letters; 513; 2-3; 2-2002; 175-178
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150301
identifier_str_mv Curatti, Leonardo; Flores, Enrique; Salerno, Graciela Lidia; Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp; Elsevier Science; FEBS Letters; 513; 2-3; 2-2002; 175-178
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/S0014-5793%2802%2902283-4?sid=nlm%3Apubmed
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0014-5793(02)02283-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981322834837504
score 12.48226

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